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  glioblastoma [Mes]

Overall DesignOperative specimens from five glioblastoma patients (MGH26, MGH28, MGH29, MGH30, MGH31) were acutely dissociated, depleted for CD45+ inflammatory cells and then sorted as single cells (576 samples). Population controls for each tumor were isolated by sorting 2000-10000 cells and processed in parallel (5 population control samples). Single cells from two established cell lines, GBM6 and GBM8, were also sorted as single cells (192 samples).
SummaryWe report transcriptomes from 430 single glioblastoma cells isolated from 5 individual tumors and 102 single cells from gliomasphere cells lines generated using SMART-seq. In addition, we report population RNA-seq from the five tumors as well as RNA-seq from cell lines derived from 3 tumors (MGH26, MGH28, MGH31) cultured under serum free (GSC) and differentiated (DGC) conditions. This dataset highlights intratumoral heterogeneity with regards to the expression of de novo derived transcriptional modules and established subtype classifiers.
Dataset viewGSE57872
PMID24925914

Samples in glioblastoma [Mes]

Displaying 1-10 of 101 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE57872GSM1395963Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_H01glioblastoma [Mes]
GSE57872GSM1395962Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_G12glioblastoma [Mes]
GSE57872GSM1395947Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_F09glioblastoma [Mes]
GSE57872GSM1395946Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_F08glioblastoma [Mes]
GSE57872GSM1395934Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_E08glioblastoma [Mes]
GSE57872GSM1395931Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_E05glioblastoma [Mes]
GSE57872GSM1395927Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_E01glioblastoma [Mes]
GSE57872GSM1395925Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_D11glioblastoma [Mes]
GSE57872GSM1395889Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_A11glioblastoma [Mes]
GSE57872GSM1395884Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH31_A06glioblastoma [Mes]

Gene rank in glioblastoma [Mes]