scRNASeqDB - CellView Site

Overall Design	Operative specimens from five glioblastoma patients (MGH26, MGH28, MGH29, MGH30, MGH31) were acutely dissociated, depleted for CD45+ inflammatory cells and then sorted as single cells (576 samples). Population controls for each tumor were isolated by sorting 2000-10000 cells and processed in parallel (5 population control samples). Single cells from two established cell lines, GBM6 and GBM8, were also sorted as single cells (192 samples).
Summary	We report transcriptomes from 430 single glioblastoma cells isolated from 5 individual tumors and 102 single cells from gliomasphere cells lines generated using SMART-seq. In addition, we report population RNA-seq from the five tumors as well as RNA-seq from cell lines derived from 3 tumors (MGH26, MGH28, MGH31) cultured under serum free (GSC) and differentiated (DGC) conditions. This dataset highlights intratumoral heterogeneity with regards to the expression of de novo derived transcriptional modules and established subtype classifiers.
Dataset view	GSE57872
PMID	24925914

Displaying 1-5 of 5 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE57872	GSM1395427	Illumina HiSeq 2500	Homo sapiens	Single cell mRNA-seq_MGH26_C05	glioblastoma [Pro+Neu]
GSE57872	GSM1395480	Illumina HiSeq 2500	Homo sapiens	Single cell mRNA-seq_MGH26_G10	glioblastoma [Pro+Neu]
GSE57872	GSM1395703	Illumina HiSeq 2500	Homo sapiens	Single cell mRNA-seq_MGH29_B05	glioblastoma [Pro+Neu]
GSE57872	GSM1395801	Illumina HiSeq 2500	Homo sapiens	Single cell mRNA-seq_MGH30_B07	glioblastoma [Pro+Neu]
GSE57872	GSM1395815	Illumina HiSeq 2500	Homo sapiens	Single cell mRNA-seq_MGH30_C09	glioblastoma [Pro+Neu]

Displaying 41-50 of 5942 results.