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  hES hiPS-derived neuron

Overall DesignPatch-Seq of 20 hESC/hiPSC-derived neurons.
SummaryThe mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming various neural circuitries, through which sense, movement, thought, and emotion are generated. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single-cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here, we report success in performing both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses (WGCNA), we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neuronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single-cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.
Dataset viewGSE77564

Samples in hES hiPS-derived neuron

Displaying 11-20 of 20 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE77564GSM2054554Illumina HiSeq 2500Homo sapiensSingle neuron 11hES hiPS-derived neuron
GSE77564GSM2054555Illumina HiSeq 2500Homo sapiensSingle neuron 12hES hiPS-derived neuron
GSE77564GSM2054556Illumina HiSeq 2500Homo sapiensSingle neuron 13hES hiPS-derived neuron
GSE77564GSM2054557Illumina HiSeq 2500Homo sapiensSingle neuron 14hES hiPS-derived neuron
GSE77564GSM2054558Illumina HiSeq 2500Homo sapiensSingle neuron 15hES hiPS-derived neuron
GSE77564GSM2054559Illumina HiSeq 2500Homo sapiensSingle neuron 16hES hiPS-derived neuron
GSE77564GSM2054560Illumina HiSeq 2500Homo sapiensSingle neuron 17hES hiPS-derived neuron
GSE77564GSM2054561Illumina HiSeq 2500Homo sapiensSingle neuron 18hES hiPS-derived neuron
GSE77564GSM2054562Illumina HiSeq 2500Homo sapiensSingle neuron 19hES hiPS-derived neuron
GSE77564GSM2054563Illumina HiSeq 2500Homo sapiensSingle neuron 20hES hiPS-derived neuron

Gene rank in hES hiPS-derived neuron

Displaying 21-30 of 19234 results.
Rank orderGene SymbolEnsembl ID