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  hES hiPS-derived neuron

Overall DesignPatch-Seq of 20 hESC/hiPSC-derived neurons.
SummaryThe mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming various neural circuitries, through which sense, movement, thought, and emotion are generated. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single-cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here, we report success in performing both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses (WGCNA), we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neuronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single-cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.
Dataset viewGSE77564
PMID26883038

Samples in hES hiPS-derived neuron

Displaying 1-10 of 20 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE77564GSM2054544Illumina HiSeq 2500Homo sapiensSingle neuron 1hES hiPS-derived neuron
GSE77564GSM2054545Illumina HiSeq 2500Homo sapiensSingle neuron 2hES hiPS-derived neuron
GSE77564GSM2054546Illumina HiSeq 2500Homo sapiensSingle neuron 3hES hiPS-derived neuron
GSE77564GSM2054547Illumina HiSeq 2500Homo sapiensSingle neuron 4hES hiPS-derived neuron
GSE77564GSM2054548Illumina HiSeq 2500Homo sapiensSingle neuron 5hES hiPS-derived neuron
GSE77564GSM2054549Illumina HiSeq 2500Homo sapiensSingle neuron 6hES hiPS-derived neuron
GSE77564GSM2054550Illumina HiSeq 2500Homo sapiensSingle neuron 7hES hiPS-derived neuron
GSE77564GSM2054551Illumina HiSeq 2500Homo sapiensSingle neuron 8hES hiPS-derived neuron
GSE77564GSM2054552Illumina HiSeq 2500Homo sapiensSingle neuron 9hES hiPS-derived neuron
GSE77564GSM2054553Illumina HiSeq 2500Homo sapiensSingle neuron 10hES hiPS-derived neuron

Gene rank in hES hiPS-derived neuron

Displaying 19201-19210 of 19234 results.
Rank orderGene SymbolEnsembl ID
19201TFAP2DENSG00000008197
19202APOBENSG00000084674
19203LGALS8-AS1ENSG00000223776
19204ST7-AS1ENSG00000227199
19205FAM81AENSG00000157470
19206GAL3ST2ENSG00000154252
19207GNRHRENSG00000109163
19208KCNK17ENSG00000124780
19209WDR64ENSG00000162843
19210CASC10ENSG00000204682