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  human K562 cells

Overall DesignA total of 8 single cell data sets are submitted: 3 for mouse embryonic stem (ES) cells (1 biological replicate, 2 technical replicates); 3 samples following LIF withdrawal (days 2,4, 7); one pure RNA data set (from human lymphoblast K562 cells); and one sample of single K562 cells.
SummaryRecently, RNA sequencing has achieved single cell resolution, but what is limiting is an effective way to routinely isolate and process large numbers of individual cells for in-depth sequencing, and to do so quantitatively. We have developed a droplet-microfluidic approach for parallel barcoding thousands of individual cells for subsequent RNA profiling by next-generation sequencing. This high-throughput method shows a surprisingly low noise profile and is readily adaptable to other sequencing-based assays. Using this technique, we analyzed mouse embryonic stem cells, revealing in detail the population structure and the heterogeneous onset of differentiation after LIF withdrawal. The reproducibility and low noise of this high-throughput single cell data allowed us to deconstruct cell populations and infer gene expression relationships.
Dataset viewGSE65525
PMID26000487

Samples in human K562 cells

Displaying 1-10 of 239 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE65525GSM1599500.1Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.2Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.3Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.4Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.5Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.6Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.7Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.8Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.9Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.10Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells

Gene rank in human K562 cells

Displaying 1-10 of 24121 results.
Rank orderGene SymbolEnsembl ID
1MIR1234
2KLF15ENSG00000163884
3EEF1A1ENSG00000156508
4MTRNR2L1ENSG00000256618
5RPS8ENSG00000142937
6RPL10ENSG00000147403
7RPS2ENSG00000140988
8MTRNR2L6ENSG00000270672
9RPS15ENSG00000115268
10MTRNR2L8ENSG00000255823