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  human K562 cells

Overall DesignA total of 8 single cell data sets are submitted: 3 for mouse embryonic stem (ES) cells (1 biological replicate, 2 technical replicates); 3 samples following LIF withdrawal (days 2,4, 7); one pure RNA data set (from human lymphoblast K562 cells); and one sample of single K562 cells.
SummaryRecently, RNA sequencing has achieved single cell resolution, but what is limiting is an effective way to routinely isolate and process large numbers of individual cells for in-depth sequencing, and to do so quantitatively. We have developed a droplet-microfluidic approach for parallel barcoding thousands of individual cells for subsequent RNA profiling by next-generation sequencing. This high-throughput method shows a surprisingly low noise profile and is readily adaptable to other sequencing-based assays. Using this technique, we analyzed mouse embryonic stem cells, revealing in detail the population structure and the heterogeneous onset of differentiation after LIF withdrawal. The reproducibility and low noise of this high-throughput single cell data allowed us to deconstruct cell populations and infer gene expression relationships.
Dataset viewGSE65525
PMID26000487

Samples in human K562 cells

Displaying 151-160 of 239 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE65525GSM1599500.234Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.235Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.236Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.237Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.238Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.239Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.24Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.25Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.26Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells
GSE65525GSM1599500.27Illumina NextSeq 500Homo sapienshuman K562 cells (239 cells)human K562 cells

Gene rank in human K562 cells

Displaying 24091-24100 of 24121 results.
Rank orderGene SymbolEnsembl ID
24091MIR1270ENSG00000238517
24092MIR323AENSG00000199069
24093MIR6072ENSG00000278069
24094MIR124-3ENSG00000207598
24095MIR1322ENSG00000283210
24096MIR3201ENSG00000266508
24097MIR4633ENSG00000264563
24098MIR4723
24099MIR487BENSG00000207754
24100MIR6505ENSG00000275770