scRNASeqDB - CellView Site

Overall Design	6 single human HepG2 cell line cells were sequenced using the newly developed scTrio-seq, other 2 HepG2 cells were sequenced using scRNA-seq and other 2 HepG2 cells were sequenced using scRRBS as technique control. 6 single mouse embryonic stem cells (mESCs) were sequenced using the newly developted scTrio-seq. Meanwhile, two scRNA-seq and two scRRBS were also completed using two mESCs separately. 26 single cells from hepatocellular carcinoma were sequenced using scTrio-seq to analyze the regulation relations between three omics of cancer cells.
Summary	Single cell genome, DNA methylome, and transcriptome sequencing has been achieved separately. However, to analyze the regulation of RNA expression by genetic and epigenetic factors within an individual cell, it is necessary to analyze these omics simultaneously from the same single cell. Here we developed a single cell triple omics sequencing technique- scTrio-seq, to analyze the genome, DNA methylome, and transcriptome concurrently of a mammalian cell.
Dataset view	GSE65364
PMID	26902283

Displaying 21-26 of 26 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE65364	GSM1593808	Illumina HiSeq 2000	Homo sapiens	Ca_21_RNA	liver cancer cell
GSE65364	GSM1593810	Illumina HiSeq 2000	Homo sapiens	Ca_22_RNA	liver cancer cell
GSE65364	GSM1593812	Illumina HiSeq 2000	Homo sapiens	Ca_23_RNA	liver cancer cell
GSE65364	GSM1593814	Illumina HiSeq 2000	Homo sapiens	Ca_24_RNA	liver cancer cell
GSE65364	GSM1593816	Illumina HiSeq 2000	Homo sapiens	Ca_25_RNA	liver cancer cell
GSE65364	GSM1593818	Illumina HiSeq 2000	Homo sapiens	Ca_26_RNA	liver cancer cell

Displaying 51-60 of 15622 results.