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  metastatic renal cell carcinoma

Overall DesignIn order to identify successful clonal propagation from patient to PDX samples and understand pathogenesis from primary to metastatic RCC, we performed whole-exome sequencing (WES, n=4) and matched aCGH (n=4) on bulk tumor samples. And we utilized single-cell RNA sequencing (scRNA-seq) to model and dissect functional heterogeneity acroass primary and metastatic RCC tumors. We checked whether of capturing live one cell, not more cells, in microfluidics by fluorescent microscopic observation. To construct RNA sequencing libraries, we performed further quality controls including adequate quantities and qualities of amplified transcriptomes respectively from single cells. Tumor cells from the parental mRCC (n=34), PDX-mRCC (n=36) and PDX-pRCC (n=46) were finally analyzed in this study after filtering out poor quality cells.
SummaryClear cell renal cell carcinoma (ccRCC) initiated from the renal epithelium is the most prevalent histological type of adult kidney cancers. Dissecting intratumoral heterogeneity (ITH) of ccRCC has leveraged to extend our knowledge on how primary tumors harboring driver mutations evolve and spread to other sites. The cellular fractions within and across the primary (pRCC) and metastatic RCC (mRCC) are heterogeneous in both their genetic and biological features determining the variability in clinical aggressiveness and sensitivity to the therapy. To achieve sustainable therapeutic benefit with targeted agents in mRCC, the effective target should focus on signaling pathways that are related to driver mutations occurred early in the clonal evolution of the disease and thus should be common to primary tumor and metastatic sites. Considering that extensive genetic heterogeneity may result in drug response variability among patients and treatment resistance, the tailored strategies for metastatic RCC is urgently needed. Here, we analyze single-cell RNA-seq (scRNA-seq) data from a matched primary RCC (pRCC) and lung metastasis (mRCC) to dissect ITH at the highest resolution to date with the objective of discovering the better therapeutic regimen.
Dataset viewGSE73121
PMID27139883

Samples in metastatic renal cell carcinoma

Displaying 11-20 of 35 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE73121GSM1887327Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_30metastatic renal cell carcinoma
GSE73121GSM1887329Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_33metastatic renal cell carcinoma
GSE73121GSM1887330Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_36metastatic renal cell carcinoma
GSE73121GSM1887331Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_39metastatic renal cell carcinoma
GSE73121GSM1887332Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_43metastatic renal cell carcinoma
GSE73121GSM1887333Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_53metastatic renal cell carcinoma
GSE73121GSM1887334Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_56metastatic renal cell carcinoma
GSE73121GSM1887335Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_57metastatic renal cell carcinoma
GSE73121GSM1887336Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_58metastatic renal cell carcinoma
GSE73121GSM1887337Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_59metastatic renal cell carcinoma

Gene rank in metastatic renal cell carcinoma

Displaying 41-50 of 18370 results.
Rank orderGene SymbolEnsembl ID
41CALM2ENSG00000143933
42CD63ENSG00000135404
43RPL36AENSG00000241343
44RPL36ENSG00000130255
45RPL9ENSG00000163682
46RPL19ENSG00000108298
47SAT1ENSG00000130066
48CRYABENSG00000109846
49RPL41P1ENSG00000227063
50GAPDHENSG00000111640