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  metastatic renal cell carcinoma

Overall DesignIn order to identify successful clonal propagation from patient to PDX samples and understand pathogenesis from primary to metastatic RCC, we performed whole-exome sequencing (WES, n=4) and matched aCGH (n=4) on bulk tumor samples. And we utilized single-cell RNA sequencing (scRNA-seq) to model and dissect functional heterogeneity acroass primary and metastatic RCC tumors. We checked whether of capturing live one cell, not more cells, in microfluidics by fluorescent microscopic observation. To construct RNA sequencing libraries, we performed further quality controls including adequate quantities and qualities of amplified transcriptomes respectively from single cells. Tumor cells from the parental mRCC (n=34), PDX-mRCC (n=36) and PDX-pRCC (n=46) were finally analyzed in this study after filtering out poor quality cells.
SummaryClear cell renal cell carcinoma (ccRCC) initiated from the renal epithelium is the most prevalent histological type of adult kidney cancers. Dissecting intratumoral heterogeneity (ITH) of ccRCC has leveraged to extend our knowledge on how primary tumors harboring driver mutations evolve and spread to other sites. The cellular fractions within and across the primary (pRCC) and metastatic RCC (mRCC) are heterogeneous in both their genetic and biological features determining the variability in clinical aggressiveness and sensitivity to the therapy. To achieve sustainable therapeutic benefit with targeted agents in mRCC, the effective target should focus on signaling pathways that are related to driver mutations occurred early in the clonal evolution of the disease and thus should be common to primary tumor and metastatic sites. Considering that extensive genetic heterogeneity may result in drug response variability among patients and treatment resistance, the tailored strategies for metastatic RCC is urgently needed. Here, we analyze single-cell RNA-seq (scRNA-seq) data from a matched primary RCC (pRCC) and lung metastasis (mRCC) to dissect ITH at the highest resolution to date with the objective of discovering the better therapeutic regimen.
Dataset viewGSE73121
PMID27139883

Samples in metastatic renal cell carcinoma

Displaying 31-35 of 35 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE73121GSM1887348Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_87metastatic renal cell carcinoma
GSE73121GSM1887349Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_88metastatic renal cell carcinoma
GSE73121GSM1887350Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_90metastatic renal cell carcinoma
GSE73121GSM1887351Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_92metastatic renal cell carcinoma
GSE73121GSM1887352Illumina HiSeq 2500Homo sapiensPt_mRCC_SC_93metastatic renal cell carcinoma

Gene rank in metastatic renal cell carcinoma

Displaying 71-80 of 18370 results.
Rank orderGene SymbolEnsembl ID
71RPS11ENSG00000142534
72NACAENSG00000196531
73RPL24ENSG00000114391
74IFITM3ENSG00000142089
75FN1ENSG00000115414
76RPS14ENSG00000164587
77ANXA5ENSG00000164111
78RPS27LENSG00000185088
79MT-ND5ENSG00000198786
80ATP5J2ENSG00000241468