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  oocyte

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in oocyte

Displaying 1-3 of 3 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160114Illumina HiSeq 2000Homo sapienshuman oocyte 4oocyte
GSE44183GSM1160113Illumina HiSeq 2000Homo sapienshuman oocyte 3oocyte
GSE44183GSM1160112Illumina HiSeq 2000Homo sapienshuman oocyte 1oocyte

Gene rank in oocyte

Displaying 14701-14710 of 14758 results.
Rank orderGene SymbolEnsembl ID
14701PLXNA3ENSG00000130827
14702PNMA5ENSG00000198883
14703PPP1R3FENSG00000049769
14704PRKXENSG00000183943
14705RAB39BENSG00000155961
14706RAB41ENSG00000147127
14707RAI2ENSG00000131831
14708RBM41ENSG00000089682
14709RBMXL3ENSG00000175718
14710RGAG1ENSG00000243978