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  single H1-Fucci cell sorted from G1 phase of the cell cycle only

Overall DesignTotal 213 H1 single cells and 247 H1-Fucci single cells were sequenced. The 213 H1 cells were used to evaluate Oscope in identifying oscillatory genes. The H1-Fucci cells were used to confirm the cell cycle gene cluster identified by Oscope in the H1 hESCs. Normalized expected counts are provided in GSE64016_H1andFUCCI_normalized_EC.csv.gz
SummaryOscillatory gene expression is fundamental to mammalian development, but technologies to monitor expression oscillations are limited. We have developed a statistical approach called Oscope to identify and characterize the transcriptional dynamics of oscillating genes in single-cell RNA-seq data from an unsynchronized cell population. Applications to a number of data sets, include a single-cell RNA-seq data set of human embroyonic stem cells (hESCs), demonstrate advantages of the approach and also identify a potential artifact in the Fluidigm C1 platform.
Dataset viewGSE64016
PMID26301841

Samples in single H1-Fucci cell sorted from G1 phase of the cell cycle only

Displaying 1-10 of 91 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE64016GSM1562699Illumina HiSeq 2500Homo sapiensG1_Exp1.091single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562698Illumina HiSeq 2500Homo sapiensG1_Exp1.090single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562697Illumina HiSeq 2500Homo sapiensG1_Exp1.089single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562696Illumina HiSeq 2500Homo sapiensG1_Exp1.088single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562695Illumina HiSeq 2500Homo sapiensG1_Exp1.087single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562694Illumina HiSeq 2500Homo sapiensG1_Exp1.086single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562693Illumina HiSeq 2500Homo sapiensG1_Exp1.085single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562692Illumina HiSeq 2500Homo sapiensG1_Exp1.084single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562691Illumina HiSeq 2500Homo sapiensG1_Exp1.083single H1-Fucci cell sorted from G1 phase of the cell cycle only
GSE64016GSM1562690Illumina HiSeq 2500Homo sapiensG1_Exp1.082single H1-Fucci cell sorted from G1 phase of the cell cycle only

Gene rank in single H1-Fucci cell sorted from G1 phase of the cell cycle only