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  single H1-Fucci cell sorted from S phase of the cell cycle only

Overall DesignTotal 213 H1 single cells and 247 H1-Fucci single cells were sequenced. The 213 H1 cells were used to evaluate Oscope in identifying oscillatory genes. The H1-Fucci cells were used to confirm the cell cycle gene cluster identified by Oscope in the H1 hESCs. Normalized expected counts are provided in GSE64016_H1andFUCCI_normalized_EC.csv.gz
SummaryOscillatory gene expression is fundamental to mammalian development, but technologies to monitor expression oscillations are limited. We have developed a statistical approach called Oscope to identify and characterize the transcriptional dynamics of oscillating genes in single-cell RNA-seq data from an unsynchronized cell population. Applications to a number of data sets, include a single-cell RNA-seq data set of human embroyonic stem cells (hESCs), demonstrate advantages of the approach and also identify a potential artifact in the Fluidigm C1 platform.
Dataset viewGSE64016
PMID26301841

Samples in single H1-Fucci cell sorted from S phase of the cell cycle only

Displaying 1-10 of 80 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE64016GSM1562700Illumina HiSeq 2500Homo sapiensS_Exp1.001single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562701Illumina HiSeq 2500Homo sapiensS_Exp1.002single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562702Illumina HiSeq 2500Homo sapiensS_Exp1.003single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562703Illumina HiSeq 2500Homo sapiensS_Exp1.004single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562704Illumina HiSeq 2500Homo sapiensS_Exp1.005single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562705Illumina HiSeq 2500Homo sapiensS_Exp1.006single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562706Illumina HiSeq 2500Homo sapiensS_Exp1.007single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562707Illumina HiSeq 2500Homo sapiensS_Exp1.008single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562708Illumina HiSeq 2500Homo sapiensS_Exp1.009single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562709Illumina HiSeq 2500Homo sapiensS_Exp1.010single H1-Fucci cell sorted from S phase of the cell cycle only

Gene rank in single H1-Fucci cell sorted from S phase of the cell cycle only

Displaying 1-10 of 17544 results.
Rank orderGene SymbolEnsembl ID
1EEF1A1ENSG00000156508
2ACTBENSG00000075624
3HSP90AB1ENSG00000096384
4MTRNR2L2ENSG00000271043
5GAPDHENSG00000111640
6NPM1ENSG00000181163
7MTRNR2L8ENSG00000255823
8ACTG1ENSG00000184009
9HSPA8ENSG00000109971
10ENO1ENSG00000074800