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  single H1-Fucci cell sorted from S phase of the cell cycle only

Overall DesignTotal 213 H1 single cells and 247 H1-Fucci single cells were sequenced. The 213 H1 cells were used to evaluate Oscope in identifying oscillatory genes. The H1-Fucci cells were used to confirm the cell cycle gene cluster identified by Oscope in the H1 hESCs. Normalized expected counts are provided in GSE64016_H1andFUCCI_normalized_EC.csv.gz
SummaryOscillatory gene expression is fundamental to mammalian development, but technologies to monitor expression oscillations are limited. We have developed a statistical approach called Oscope to identify and characterize the transcriptional dynamics of oscillating genes in single-cell RNA-seq data from an unsynchronized cell population. Applications to a number of data sets, include a single-cell RNA-seq data set of human embroyonic stem cells (hESCs), demonstrate advantages of the approach and also identify a potential artifact in the Fluidigm C1 platform.
Dataset viewGSE64016
PMID26301841

Samples in single H1-Fucci cell sorted from S phase of the cell cycle only

Displaying 21-30 of 80 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE64016GSM1562720Illumina HiSeq 2500Homo sapiensS_Exp1.021single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562721Illumina HiSeq 2500Homo sapiensS_Exp1.022single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562722Illumina HiSeq 2500Homo sapiensS_Exp1.023single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562723Illumina HiSeq 2500Homo sapiensS_Exp1.025single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562724Illumina HiSeq 2500Homo sapiensS_Exp1.026single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562725Illumina HiSeq 2500Homo sapiensS_Exp1.027single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562726Illumina HiSeq 2500Homo sapiensS_Exp1.028single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562727Illumina HiSeq 2500Homo sapiensS_Exp1.029single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562728Illumina HiSeq 2500Homo sapiensS_Exp1.030single H1-Fucci cell sorted from S phase of the cell cycle only
GSE64016GSM1562729Illumina HiSeq 2500Homo sapiensS_Exp1.031single H1-Fucci cell sorted from S phase of the cell cycle only

Gene rank in single H1-Fucci cell sorted from S phase of the cell cycle only

Displaying 11-20 of 17544 results.
Rank orderGene SymbolEnsembl ID
11PPP1CBENSG00000213639
12SETENSG00000119335
13TUBA1BENSG00000123416
14HSP90AA1ENSG00000080824
15HNRNPA1ENSG00000135486
16LDHBENSG00000111716
17PTMAENSG00000187514
18RPL3ENSG00000100316
19L1TD1ENSG00000240563
20TUBBENSG00000183311