| Tag | Content |
|---|---|
| Uniprot ID | O09053; O09050; Q80YP9; Q9JKD4; Q9Z241; Q9Z242; |
| Entrez ID | 22427 |
| Genbank protein ID | AAH60700.1; BAA20270.1; AAC78077.1; AAF64490.1; BAA20269.1; AAH50921.1; AAC72359.1; |
| Genbank nucleotide ID | XM_006509091.3; NM_011721.4; XM_017312662.1; NM_001122822.1; |
| Ensembl protein ID | ENSMUSP00000033991; ENSMUSP00000033990; |
| Ensembl nucleotide ID | ENSMUSG00000031583 |
| Gene name | Werner syndrome ATP-dependent helicase homolog |
| Gene symbol | Wrn |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | |
| Data sources | Manually collected |
| Reference | 25807483 |
| Functional description | Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (By similarity). |
| Sequence | METTSLQRKF PEWMSMQSQR CATEEKACVQ KSVLEDNLPF LEFPGSIVYS YEASDCSFLS 60 EDISMRLSDG DVVGFDMEWP PIYKPGKRSR VAVIQLCVSE SKCYLFHISS MSVFPQGLKM 120 LLENKSIKKA GVGIEGDQWK LLRDFDVKLE SFVELTDVAN EKLKCAETWS LNGLVKHVLG 180 KQLLKDKSIR CSNWSNFPLT EDQKLYAATD AYAGLIIYQK LGNLGDTAQV FALNKAEENL 240 PLEMKKQLNS ISEEMRDLAN RFPVTCRNLE TLQRVPVILK SISENLCSLR KVICGPTNTE 300 TRLKPGSSFN LLSSEDSAAA GEKEKQIGKH STFAKIKEEP WDPELDSLVK QEEVDVFRNQ 360 VKQEKGESEN EIEDNLLRED MERTCVIPSI SENELQDLEQ QAKEEKYNDV SHQLSEHLSP 420 NDDENDSSYI IESDEDLEME MLKSLENLNS DVVEPTHSTW LEMGTNGRLP PEEEDGHGNE 480 AIKEEQEEED HLLPEPNAKQ INCLKTYFGH SSFKPVQWKV IHSVLEERRD NVVVMATGYG 540 KSLCFQYPPV YTGKIGIVIS PLISLMEDQV LQLELSNVPA CLLGSAQSKN ILGDVKLGKY 600 RVIYITPEFC SGNLDLLQQL DSSIGITLIA VDEAHCISEW GHDFRSSFRM LGSLKTALPL 660 VPVIALSATA SSSIREDIIS CLNLKDPQIT CTGFDRPNLY LEVGRKTGNI LQDLKPFLVR 720 KASSAWEFEG PTIIYCPSRK MTEQVTAELG KLNLACRTYH AGMKISERKD VHHRFLRDEI 780 QCVVATVAFG MGINKADIRK VIHYGAPKEM ESYYQEIGRA GRDGLQSSCH LLWAPADFNT 840 SRNLLIEIHD EKFRLYKLKM MVKMEKYLHS SQCRRRIILS HFEDKCLQKA SLDIMGTEKC 900 CDNCRPRLNH CLTANNSEDA SQDFGPQAFQ LLSAVDILQE KFGIGIPILF LRGSNSQRLP 960 DKYRGHRLFG AGKEQAESWW KTLSHHLIAE GFLVEVPKEN KYIKTCSLTK KGRKWLGEAS 1020 SQSPPSLLLQ ANEEMFPRKV LLPSSNPVSP ETTQHSSNQN PAGLTTKQSN LERTHSYKVP 1080 EKVSSGTNIP KKSAVMPSPG TSSSPLEPAI SAQELDARTG LYARLVEARQ KHANKMDVPP 1140 AILATNKVLL DMAKMRPTTV ENMKQIDGVS EGKAALLAPL LEVIKHFCQV TSVQTDLLSS 1200 AKPHKEQEKS QEMEKKDCSL PQSVAVTYTL FQEKKMPLHS IAENRLLPLT AAGMHLAQAV 1260 KAGYPLDMER AGLTPETWKI IMDVIRNPPI NSDMYKVKLI RMLVPENLDT YLIHMAIEIL 1320 QSGSDSRTQP PCDSSRKRRF PSSAESCESC KESKEAVTET KASSSESKRK LPEWFAKGNV 1380 PSADTGSSSS MAKTKKKGLF S 1401 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0000287 | magnesium ion binding | ISS |
| GO:0000287 | magnesium ion binding | ISO |
| GO:0000400 | four-way junction DNA binding | ISO |
| GO:0000403 | Y-form DNA binding | ISO |
| GO:0000405 | bubble DNA binding | ISO |
| GO:0003677 | DNA binding | ISO |
| GO:0003678 | DNA helicase activity | ISO |
| GO:0003682 | chromatin binding | ISO |
| GO:0004386 | helicase activity | ISO |
| GO:0004527 | exonuclease activity | ISO |
| GO:0005515 | protein binding | IPI |
| GO:0005524 | ATP binding | IEA |
| GO:0008408 | 3'-5' exonuclease activity | ISO |
| GO:0008408 | 3'-5' exonuclease activity | ISS |
| GO:0008408 | 3'-5' exonuclease activity | IDA |
| GO:0009378 | four-way junction helicase activity | ISO |
| GO:0009378 | four-way junction helicase activity | IBA |
| GO:0016887 | ATPase activity | ISO |
| GO:0030145 | manganese ion binding | ISS |
| GO:0030145 | manganese ion binding | ISO |
| GO:0032405 | MutLalpha complex binding | ISO |
| GO:0042803 | protein homodimerization activity | ISO |
| GO:0043138 | 3'-5' DNA helicase activity | ISO |
| GO:0043138 | 3'-5' DNA helicase activity | IBA |
| GO:0044877 | protein-containing complex binding | ISO |
| GO:0051880 | G-quadruplex DNA binding | ISO |
| GO:0061749 | forked DNA-dependent helicase activity | ISO |
| GO:0061821 | telomeric D-loop binding | ISO |
| GO:0070337 | 3'-flap-structured DNA binding | ISO |
| GO:1905773 | 8-hydroxy-2'-deoxyguanosine DNA binding | ISO |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0000723 | telomere maintenance | ISO |
| GO:0000723 | telomere maintenance | IMP |
| GO:0000723 | telomere maintenance | IGI |
| GO:0000723 | telomere maintenance | IBA |
| GO:0000724 | double-strand break repair via homologous recombination | IBA |
| GO:0000731 | DNA synthesis involved in DNA repair | ISO |
| GO:0001302 | replicative cell aging | IMP |
| GO:0006259 | DNA metabolic process | ISS |
| GO:0006259 | DNA metabolic process | ISO |
| GO:0006259 | DNA metabolic process | IMP |
| GO:0006260 | DNA replication | ISO |
| GO:0006260 | DNA replication | IGI |
| GO:0006260 | DNA replication | IMP |
| GO:0006268 | DNA unwinding involved in DNA replication | IBA |
| GO:0006281 | DNA repair | IBA |
| GO:0006284 | base-excision repair | ISO |
| GO:0006302 | double-strand break repair | ISS |
| GO:0006302 | double-strand break repair | ISO |
| GO:0006310 | DNA recombination | IBA |
| GO:0006974 | cellular response to DNA damage stimulus | ISO |
| GO:0006979 | response to oxidative stress | ISO |
| GO:0007420 | brain development | IEA |
| GO:0007569 | cell aging | ISO |
| GO:0009267 | cellular response to starvation | ISO |
| GO:0010225 | response to UV-C | ISO |
| GO:0010259 | multicellular organism aging | ISO |
| GO:0010259 | multicellular organism aging | IGI |
| GO:0010259 | multicellular organism aging | IBA |
| GO:0031297 | replication fork processing | ISO |
| GO:0032508 | DNA duplex unwinding | ISO |
| GO:0032508 | DNA duplex unwinding | IBA |
| GO:0040009 | regulation of growth rate | IMP |
| GO:0042981 | regulation of apoptotic process | ISO |
| GO:0044806 | G-quadruplex DNA unwinding | ISO |
| GO:0044806 | G-quadruplex DNA unwinding | IBA |
| GO:0044806 | G-quadruplex DNA unwinding | IGI |
| GO:0044806 | G-quadruplex DNA unwinding | IMP |
| GO:0051345 | positive regulation of hydrolase activity | ISO |
| GO:0061820 | telomeric D-loop disassembly | ISO |
| GO:0071480 | cellular response to gamma radiation | ISS |
| GO:0071480 | cellular response to gamma radiation | ISO |
| GO:0090305 | nucleic acid phosphodiester bond hydrolysis | IEA |
| GO:0098530 | positive regulation of strand invasion | ISO |
| GO:1902570 | protein localization to nucleolus | ISO |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0000781 | chromosome, telomeric region | ISO |
| GO:0000781 | chromosome, telomeric region | IMP |
| GO:0005634 | nucleus | IBA |
| GO:0005634 | nucleus | IDA |
| GO:0005654 | nucleoplasm | ISO |
| GO:0005654 | nucleoplasm | IDA |
| GO:0005654 | nucleoplasm | IBA |
| GO:0005657 | replication fork | IMP |
| GO:0005694 | chromosome | IBA |
| GO:0005730 | nucleolus | ISO |
| GO:0005730 | nucleolus | ISS |
| GO:0005737 | cytoplasm | IBA |
| GO:0005813 | centrosome | ISO |
| GO:0016607 | nuclear speck | ISO |
| GO:0043005 | neuron projection | ISO |
| GO:0005730 | nucleolus | IDA |
| Reactome ID | Reactome Term | Evidence |
|---|---|---|
| R-MMU-1640170 | Cell Cycle | IEA |
| R-MMU-212436 | Generic Transcription Pathway | IEA |
| R-MMU-3700989 | Transcriptional Regulation by TP53 | IEA |
| R-MMU-5633007 | Regulation of TP53 Activity | IEA |
| R-MMU-5685938 | HDR through Single Strand Annealing (SSA) | IEA |
| R-MMU-5685942 | HDR through Homologous Recombination (HRR) | IEA |
| R-MMU-5693532 | DNA Double-Strand Break Repair | IEA |
| R-MMU-5693537 | Resolution of D-Loop Structures | IEA |
| R-MMU-5693538 | Homology Directed Repair | IEA |
| R-MMU-5693554 | Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) | IEA |
| R-MMU-5693567 | HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) | IEA |
| R-MMU-5693568 | Resolution of D-loop Structures through Holliday Junction Intermediates | IEA |
| R-MMU-5693579 | Homologous DNA Pairing and Strand Exchange | IEA |
| R-MMU-5693607 | Processing of DNA double-strand break ends | IEA |
| R-MMU-5693616 | Presynaptic phase of homologous DNA pairing and strand exchange | IEA |
| R-MMU-6804756 | Regulation of TP53 Activity through Phosphorylation | IEA |
| R-MMU-69473 | G2/M DNA damage checkpoint | IEA |
| R-MMU-69481 | G2/M Checkpoints | IEA |
| R-MMU-69620 | Cell Cycle Checkpoints | IEA |
| R-MMU-73857 | RNA Polymerase II Transcription | IEA |
| R-MMU-73894 | DNA Repair | IEA |
| R-MMU-74160 | Gene expression (Transcription) | IEA |
| Keyword ID | Keyword Term |
|---|---|
| KW-0002 | 3D-structure |
| KW-0067 | ATP-binding |
| KW-0227 | DNA damage |
| KW-0234 | DNA repair |
| KW-0238 | DNA-binding |
| KW-0269 | Exonuclease |
| KW-0347 | Helicase |
| KW-0378 | Hydrolase |
| KW-1017 | Isopeptide bond |
| KW-0460 | Magnesium |
| KW-0479 | Metal-binding |
| KW-0511 | Multifunctional enzyme |
| KW-0540 | Nuclease |
| KW-0547 | Nucleotide-binding |
| KW-0539 | Nucleus |
| KW-0597 | Phosphoprotein |
| KW-1185 | Reference proteome |
| KW-0832 | Ubl conjugation |
| KW-0862 | Zinc |
| InterPro ID | InterPro Term |
|---|---|
| IPR002562 | 3'-5'_exonuclease_dom |
| IPR011545 | DEAD/DEAH_box_helicase_dom |
| IPR004589 | DNA_helicase_ATP-dep_RecQ |
| IPR014001 | Helicase_ATP-bd |
| IPR001650 | Helicase_C |
| IPR029491 | Helicase_HTH |
| IPR010997 | HRDC-like_sf |
| IPR002121 | HRDC_dom |
| IPR027417 | P-loop_NTPase |
| IPR032284 | RecQ_Zn-bd |
| IPR012337 | RNaseH-like_sf |
| IPR036397 | RNaseH_sf |
| IPR018982 | RQC_domain |
| IPR036388 | WH-like_DNA-bd_sf |
| IPR036390 | WH_DNA-bd_sf |