| Tag | Content |
|---|---|
| Uniprot ID | O35625 |
| Entrez ID | 12005 |
| Genbank protein ID | AAC53285.1 |
| Genbank nucleotide ID | NM_009733.2 |
| Ensembl protein ID | |
| Ensembl nucleotide ID | |
| Gene name | Axin-1 |
| Gene symbol | Axin1 |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | |
| Data sources | Manually collected |
| Reference | 19204372 |
| Functional description | Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. |
| Sequence | MNVQEQGFPL DLGASFTEDA PRPPVPGEEG ELVSTDSRPV NHSFCSGKGT SIKSETSTAT 60 PRRSDLDLGY EPEGSASPTP PYLRWAESLH SLLDDQDGIS LFRTFLKQEG CADLLDFWFA 120 CSGFRKLEPC DSNEEKRLKL ARAIYRKYIL DSNGIVSRQT KPATKSFIKD CVMKQQIDPA 180 MFDQAQTEIQ STMEENTYPS FLKSDIYLEY TRTGSESPKV CSDQSSGSGT GKGMSGYLPT 240 LNEDEEWKCD QDADEDDGRD PLPPSRLTQK LLLETAAPRA PSSRRYNEGR ELRYGSWREP 300 VNPYYVNSGY ALAPATSAND SEQQSLSSDA DTLSLTDSSV DGIPPYRIRK QHRREMQESI 360 QVNGRVPLPH IPRTYRMPKE IRVEPQKFAE ELIHRLEAVQ RTREAEEKLE ERLKRVRMEE 420 EGEDGEMPSG PMASHKLPSV PAWHHFPPRY VDMGCSGLRD AHEENPESIL DEHVQRVMRT 480 PGCQSPGPGH RSPDSGHVAK TAVLGGTASG HGKHVPKLGL KLDTAGLHHH RHVHHHVHHN 540 SARPKEQMEA EVARRVQSSF SWGPETHGHA KPRSYSENAG TTLSAGDLAF GGKTSAPSKR 600 NTKKAESGKN ANAEVPSTTE DAEKNQKIMQ WIIEGEKEIS RHRKAGHGSS GLRKQQAHES 660 SRPLSIERPG AVHPWVSAQL RNSVQPSHLF IQDPTMPPNP APNPLTQLEE ARRRLEEEEK 720 RANKLPSKQR YVQAVMQRGR TCVRPACAPV LSVVPAVSDL ELSETETKSQ RKAGGGSAPP 780 CDSIVVAYYF CGEPIPYRTL VRGRAVTLGQ FKELLTKKGS YRYYFKKVSD EFDCGVVFEE 840 VREDEAVLPV FEEKIIGKVE KVD 863 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0002039 | p53 binding | IPI |
| GO:0005102 | signaling receptor binding | ISO |
| GO:0005515 | protein binding | IPI |
| GO:0008013 | beta-catenin binding | ISO |
| GO:0008013 | beta-catenin binding | IPI |
| GO:0008022 | protein C-terminus binding | IDA |
| GO:0008022 | protein C-terminus binding | IPI |
| GO:0019899 | enzyme binding | ISO |
| GO:0019901 | protein kinase binding | ISO |
| GO:0019901 | protein kinase binding | IPI |
| GO:0019904 | protein domain specific binding | ISO |
| GO:0019904 | protein domain specific binding | IPI |
| GO:0031625 | ubiquitin protein ligase binding | ISO |
| GO:0031625 | ubiquitin protein ligase binding | IPI |
| GO:0035591 | signaling adaptor activity | ISO |
| GO:0042802 | identical protein binding | ISO |
| GO:0042802 | identical protein binding | IPI |
| GO:0042803 | protein homodimerization activity | IPI |
| GO:0043621 | protein self-association | IPI |
| GO:0046332 | SMAD binding | ISO |
| GO:0060090 | molecular adaptor activity | ISO |
| GO:0060090 | molecular adaptor activity | IMP |
| GO:0070016 | armadillo repeat domain binding | ISO |
| GO:0070411 | I-SMAD binding | ISO |
| GO:0070411 | I-SMAD binding | IDA |
| GO:0070411 | I-SMAD binding | IPI |
| GO:0070412 | R-SMAD binding | IPI |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0000209 | protein polyubiquitination | IDA |
| GO:0001701 | in utero embryonic development | IGI |
| GO:0001701 | in utero embryonic development | IMP |
| GO:0001932 | regulation of protein phosphorylation | IGI |
| GO:0001934 | positive regulation of protein phosphorylation | ISO |
| GO:0001934 | positive regulation of protein phosphorylation | IDA |
| GO:0006913 | nucleocytoplasmic transport | IDA |
| GO:0006915 | apoptotic process | IEA |
| GO:0007605 | sensory perception of sound | IMP |
| GO:0009950 | dorsal/ventral axis specification | IDA |
| GO:0009950 | dorsal/ventral axis specification | ISO |
| GO:0009953 | dorsal/ventral pattern formation | ISO |
| GO:0009953 | dorsal/ventral pattern formation | IMP |
| GO:0010629 | negative regulation of gene expression | IDA |
| GO:0010800 | positive regulation of peptidyl-threonine phosphorylation | IDA |
| GO:0016055 | Wnt signaling pathway | IDA |
| GO:0016055 | Wnt signaling pathway | IPI |
| GO:0030163 | protein catabolic process | IDA |
| GO:0030178 | negative regulation of Wnt signaling pathway | IDA |
| GO:0030511 | positive regulation of transforming growth factor beta receptor signaling pathway | IDA |
| GO:0030511 | positive regulation of transforming growth factor beta receptor signaling pathway | IGI |
| GO:0030511 | positive regulation of transforming growth factor beta receptor signaling pathway | IMP |
| GO:0031122 | cytoplasmic microtubule organization | IGI |
| GO:0031398 | positive regulation of protein ubiquitination | IDA |
| GO:0031398 | positive regulation of protein ubiquitination | IGI |
| GO:0031398 | positive regulation of protein ubiquitination | IMP |
| GO:0032147 | activation of protein kinase activity | ISO |
| GO:0032436 | positive regulation of proteasomal ubiquitin-dependent protein catabolic process | IDA |
| GO:0033138 | positive regulation of peptidyl-serine phosphorylation | IDA |
| GO:0034244 | negative regulation of transcription elongation from RNA polymerase II promoter | IDA |
| GO:0034622 | cellular protein-containing complex assembly | ISO |
| GO:0036342 | post-anal tail morphogenesis | IMP |
| GO:0043507 | positive regulation of JUN kinase activity | IDA |
| GO:0043507 | positive regulation of JUN kinase activity | IMP |
| GO:0043507 | positive regulation of JUN kinase activity | ISO |
| GO:0045599 | negative regulation of fat cell differentiation | IDA |
| GO:0045732 | positive regulation of protein catabolic process | ISO |
| GO:0045860 | positive regulation of protein kinase activity | ISO |
| GO:0045860 | positive regulation of protein kinase activity | IDA |
| GO:0045893 | positive regulation of transcription, DNA-templated | ISO |
| GO:0045893 | positive regulation of transcription, DNA-templated | IDA |
| GO:0046330 | positive regulation of JNK cascade | IMP |
| GO:0048318 | axial mesoderm development | IMP |
| GO:0048320 | axial mesoderm formation | IMP |
| GO:0051248 | negative regulation of protein metabolic process | IDA |
| GO:0051443 | positive regulation of ubiquitin-protein transferase activity | ISO |
| GO:0051443 | positive regulation of ubiquitin-protein transferase activity | IMP |
| GO:0060070 | canonical Wnt signaling pathway | IDA |
| GO:0060070 | canonical Wnt signaling pathway | IMP |
| GO:0060322 | head development | IMP |
| GO:0060828 | regulation of canonical Wnt signaling pathway | IDA |
| GO:0071514 | genetic imprinting | IMP |
| GO:0090090 | negative regulation of canonical Wnt signaling pathway | ISO |
| GO:0090090 | negative regulation of canonical Wnt signaling pathway | IDA |
| GO:2000060 | positive regulation of ubiquitin-dependent protein catabolic process | ISO |
| GO:2000060 | positive regulation of ubiquitin-dependent protein catabolic process | IDA |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0005634 | nucleus | ISO |
| GO:0005737 | cytoplasm | ISO |
| GO:0005737 | cytoplasm | IDA |
| GO:0005829 | cytosol | TAS |
| GO:0005886 | plasma membrane | ISO |
| GO:0005886 | plasma membrane | IDA |
| GO:0005886 | plasma membrane | NAS |
| GO:0005938 | cell cortex | IDA |
| GO:0014069 | postsynaptic density | ISO |
| GO:0016328 | lateral plasma membrane | ISO |
| GO:0030877 | beta-catenin destruction complex | ISO |
| GO:0030877 | beta-catenin destruction complex | IDA |
| GO:0030877 | beta-catenin destruction complex | TAS |
| GO:0031410 | cytoplasmic vesicle | ISO |
| GO:0031410 | cytoplasmic vesicle | IDA |
| GO:0032991 | protein-containing complex | IDA |
| GO:0048471 | perinuclear region of cytoplasm | ISO |
| GO:0071944 | cell periphery | ISO |
| GO:1990909 | Wnt signalosome | IDA |
| GO:0005794 | Golgi apparatus | ISO |
| GO:0005881 | cytoplasmic microtubule | IDA |
| Reactome ID | Reactome Term | Evidence |
|---|---|---|
| R-MMU-162582 | Signal Transduction | IEA |
| R-MMU-195253 | Degradation of beta-catenin by the destruction complex | IEA |
| R-MMU-195721 | Signaling by WNT | IEA |
| R-MMU-196299 | Beta-catenin phosphorylation cascade | IEA |
| R-MMU-201681 | TCF dependent signaling in response to WNT | IEA |
| R-MMU-392499 | Metabolism of proteins | IEA |
| R-MMU-4641257 | Degradation of AXIN | IEA |
| R-MMU-4641262 | Disassembly of the destruction complex and recruitment of AXIN to the membrane | IEA |
| R-MMU-5688426 | Deubiquitination | IEA |
| R-MMU-5689880 | Ub-specific processing proteases | IEA |
| R-MMU-597592 | Post-translational protein modification | IEA |
| Keyword ID | Keyword Term |
|---|---|
| KW-0002 | 3D-structure |
| KW-0013 | ADP-ribosylation |
| KW-0025 | Alternative splicing |
| KW-0053 | Apoptosis |
| KW-1003 | Cell membrane |
| KW-0963 | Cytoplasm |
| KW-0217 | Developmental protein |
| KW-1017 | Isopeptide bond |
| KW-0472 | Membrane |
| KW-0539 | Nucleus |
| KW-0597 | Phosphoprotein |
| KW-1185 | Reference proteome |
| KW-0043 | Tumor suppressor |
| KW-0832 | Ubl conjugation |
| KW-0879 | Wnt signaling pathway |