| Tag | Content |
|---|---|
| Uniprot ID | O89026 |
| Entrez ID | 19876 |
| Genbank protein ID | CAA76850.1 |
| Genbank nucleotide ID | NM_019413.2 |
| Ensembl protein ID | |
| Ensembl nucleotide ID | |
| Gene name | Roundabout homolog 1 |
| Gene symbol | Robo1 |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | |
| Data sources | Manually collected |
| Reference | 25807483 |
| Functional description | Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10433822, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (By similarity). May be required for lung development (PubMed:11734623). |
| Sequence | MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC KAEGRPTPTI 60 EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR PDEGVYICVA RNYLGEAVSH 120 NASLEVAILR DDFRQNPSDV MVAVGEPAVM ECQPPRGHPE PTISWKKDGS PLDDKDERIT 180 IRGGKLMITY TRKSDAGKYV CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA 240 EFKCEARGDP VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA 300 EASATLTVQE PPHFVVKPRD QVVALGRTVT FQCEATGNPQ PAIFWRREGS QNLLFSYQPP 360 QSSSRFSVSQ TGDLTITNVQ RSDVGYYICQ TLNVAGSIIT KAYLEVTDVI ADRPPPVIRQ 420 GPVNQTVAVD GTLILSCVAT GSPAPTILWR KDGVLVSTQD SRIKQLESGV LQIRYAKLGD 480 TGRYTCTAST PSGEATWSAY IEVQEFGVPV QPPRPTDPNL IPSAPSKPEV TDVSKNTVTL 540 SWQPNLNSGA TPTSYIIEAF SHASGSSWQT AAENVKTETF AIKGLKPNAI YLFLVRAANA 600 YGISDPSQIS DPVKTQDVPP TSQGVDHKQV QRELGNVVLH LHNPTILSSS SVEVHWTVDQ 660 QSQYIQGYKI LYRPSGASHG ESEWLVFEVR TPTKNSVVIP DLRKGVNYEI KARPFFNEFQ 720 GADSEIKFAK TLEEAPSAPP RSVTVSKNDG NGTAILVTWQ PPPEDTQNGM VQEYKVWCLG 780 NETKYHINKT VDGSTFSVVI PSLVPGIRYS VEVAASTGAG PGVKSEPQFI QLDSHGNPVS 840 PEDQVSLAQQ ISDVVRQPAF IAGIGAACWI ILMVFSIWLY RHRKKRNGLT STYAGIRKVP 900 SFTFTPTVTY QRGGEAVSSG GRPGLLNISE PATQPWLADT WPNTGNNHND CSINCCTAGN 960 GNSDSNLTTY SRPADCIANY NNQLDNKQTN LMLPESTVYG DVDLSNKINE MKTFNSPNLK 1020 DGRFVNPSGQ PTPYATTQLI QANLSNNMNN GAGDSSEKHW KPPGQQKPEV APIQYNIMEQ 1080 NKLNKDYRAN DTIPPTIPYN QSYDQNTGGS YNSSDRGSST SGSQGHKKGA RTPKAPKQGG 1140 MNWADLLPPP PAHPPPHSNS EEYNMSVDES YDQEMPCPVP PAPMYLQQDE LQEEEDERGP 1200 TPPVRGAASS PAAVSYSHQS TATLTPSPQE ELQPMLQDCP EDLGHMPHPP DRRRQPVSPP 1260 PPPRPISPPH TYGYISGPLV SDMDTDAPEE EEDEADMEVA KMQTRRLLLR GLEQTPASSV 1320 GDLESSVTGS MINGWGSASE EDNISSGRSS VSSSDGSFFT DADFAQAVAA AAEYAGLKVA 1380 RRQMQDAAGR RHFHASQCPR PTSPVSTDSN MSAVVIQKAR PAKKQKHQPG HLRREAYADD 1440 LPPPPVPPPA IKSPTVQSKA QLEVRPVMVP KLASIEARTD RSSDRKGGSY KGREALDGRQ 1500 VTDLRTNPSD PREAQEQPND GKGRGTRQPK RDLPPAKTHL GQEDILPYCR PTFPTSNNPR 1560 DPSSSSSMSS RGSGSRQREQ ANVGRRNMAE MQVLGGFERG DENNEELEET ES 1612 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0005515 | protein binding | IPI |
| GO:0008046 | axon guidance receptor activity | ISO |
| GO:0030275 | LRR domain binding | ISO |
| GO:0042802 | identical protein binding | ISO |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0002042 | cell migration involved in sprouting angiogenesis | ISO |
| GO:0003148 | outflow tract septum morphogenesis | IGI |
| GO:0003180 | aortic valve morphogenesis | IGI |
| GO:0003184 | pulmonary valve morphogenesis | IGI |
| GO:0003272 | endocardial cushion formation | IGI |
| GO:0003281 | ventricular septum development | IMP |
| GO:0006919 | activation of cysteine-type endopeptidase activity involved in apoptotic process | ISO |
| GO:0007156 | homophilic cell adhesion via plasma membrane adhesion molecules | ISO |
| GO:0007411 | axon guidance | ISO |
| GO:0007411 | axon guidance | IMP |
| GO:0007411 | axon guidance | IGI |
| GO:0007507 | heart development | IMP |
| GO:0008285 | negative regulation of cell population proliferation | IMP |
| GO:0010628 | positive regulation of gene expression | IDA |
| GO:0010629 | negative regulation of gene expression | IDA |
| GO:0016199 | axon midline choice point recognition | IGI |
| GO:0021836 | chemorepulsion involved in postnatal olfactory bulb interneuron migration | ISO |
| GO:0021891 | olfactory bulb interneuron development | IGI |
| GO:0030336 | negative regulation of cell migration | ISS |
| GO:0030336 | negative regulation of cell migration | ISO |
| GO:0033600 | negative regulation of mammary gland epithelial cell proliferation | ISO |
| GO:0035025 | positive regulation of Rho protein signal transduction | ISS |
| GO:0035025 | positive regulation of Rho protein signal transduction | ISO |
| GO:0035385 | Roundabout signaling pathway | ISS |
| GO:0035385 | Roundabout signaling pathway | ISO |
| GO:0035481 | positive regulation of Notch signaling pathway involved in heart induction | IDA |
| GO:0035904 | aorta development | IGI |
| GO:0043406 | positive regulation of MAP kinase activity | ISO |
| GO:0048814 | regulation of dendrite morphogenesis | ISO |
| GO:0050772 | positive regulation of axonogenesis | ISO |
| GO:0050925 | negative regulation of negative chemotaxis | ISO |
| GO:0060412 | ventricular septum morphogenesis | IGI |
| GO:0060763 | mammary duct terminal end bud growth | IMP |
| GO:0060976 | coronary vasculature development | IMP |
| GO:0070100 | negative regulation of chemokine-mediated signaling pathway | ISO |
| GO:1900748 | positive regulation of vascular endothelial growth factor signaling pathway | ISO |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0005737 | cytoplasm | ISO |
| GO:0005886 | plasma membrane | ISO |
| GO:0005886 | plasma membrane | TAS |
| GO:0009986 | cell surface | ISO |
| GO:0016021 | integral component of membrane | IEA |
| GO:0030424 | axon | ISO |
| GO:0030673 | axolemma | IDA |
| GO:0033116 | endoplasmic reticulum-Golgi intermediate compartment membrane | IEA |
| GO:0043025 | neuronal cell body | ISO |
| Reactome ID | Reactome Term | Evidence |
|---|---|---|
| R-MMU-1266738 | Developmental Biology | IEA |
| R-MMU-376176 | Signaling by ROBO receptors | IEA |
| R-MMU-422475 | Axon guidance | IEA |
| R-MMU-428890 | Role of ABL in ROBO-SLIT signaling | IEA |
| R-MMU-8985586 | SLIT2:ROBO1 increases RHOA activity | IEA |
| R-MMU-9010553 | Regulation of expression of SLITs and ROBOs | IEA |
| Keyword ID | Keyword Term |
|---|---|
| KW-1003 | Cell membrane |
| KW-0966 | Cell projection |
| KW-0145 | Chemotaxis |
| KW-0217 | Developmental protein |
| KW-0221 | Differentiation |
| KW-1015 | Disulfide bond |
| KW-0325 | Glycoprotein |
| KW-0393 | Immunoglobulin domain |
| KW-0472 | Membrane |
| KW-0524 | Neurogenesis |
| KW-0597 | Phosphoprotein |
| KW-0675 | Receptor |
| KW-1185 | Reference proteome |
| KW-0677 | Repeat |
| KW-0732 | Signal |
| KW-0812 | Transmembrane |
| KW-1133 | Transmembrane helix |
| KW-0832 | Ubl conjugation |