| Tag | Content |
|---|---|
| Uniprot ID | Q9EQH7; Q6AXE0; Q9D557; |
| Entrez ID | 83398 |
| Genbank protein ID | AAH79622.1; AAG34793.1; BAE24894.1; BAB29967.1; |
| Genbank nucleotide ID | NM_031186.3; NM_001293682.1; |
| Ensembl protein ID | ENSMUSP00000118796 |
| Ensembl nucleotide ID | ENSMUSG00000027977 |
| Gene name | Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3 |
| Gene symbol | Ndst3 |
| Organism | Mus musculus |
| NCBI taxa ID | 10090 |
| Cleft type | CPO |
| Developmental stage | |
| Data sources | Manually collected |
| Reference | 18385133 |
| Functional description | Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity. |
| Sequence | MSFIMKPHRH FQRTLILLAT FCMVSIIISA YYLYSGYKQE SEVSGRASEV DCGDLQHIPS 60 RLMEVRRTMI SDASRTDPTV LVFVESQYSS LGQDIIMMLE SIRFHYHTEI APGKGDLPAL 120 TDNVKGKYVL IIYENILKYI NMDSWNRSLL DKYCIEYGVG IIGFHKTSEK NLQSFQFRGF 180 PFSISGNLAV KDCCINPHSP LLRVTKSSKL DRGSLPGTDW TVFQINHSTY QPVIFAKVKT 240 PENLSPPISK HAFYATIIHD LGLHDGIQRV LFGNNLNFWL HKLIFIDAIS FLSGKRLTLS 300 LDRYILVDID DIFVGKEGTR MNTNDVKALL DTQNLLRTQI TNFTFNLGFS GKFYHTGTEE 360 EDEGDDCLLG SVDEFWWFPH MWSHMQPHLF HNESSLIEQM ILNKKFALEH GIPTDMGYAV 420 SPHHSGVYPV HVQLYEAWKK VWNIKITSTE EYPHLKPARY RRGFIHKNIM VLPRQTCGLF 480 THTIFYKEYP GGPRELDKSI HGGELFFTVV LNPISIFMTH LSNYGNDRLG LYTFVNLANF 540 VQTWTNLRLQ TLPPAQLAHK YFELFPDQKD PLWQNPCDDK RHRDIWSKEK TCDRLPKFLV 600 IGPQKTGTTA LCLFLIMHPS ILSNSPSPKS FEEVQFFNRN NYHRGIDWYM DFFPVPSNVT 660 TDFLFEKSAN YFHSEDAPKR AASLVPKAKI ITILIDPSDR AYSWYQHQRS HEDPAALKFS 720 FYEVISAGPN APWELRTLQK RCLVPGWYAN HIERWLVYFP PFQLLIIDGQ HLRTTPATVM 780 DEVQKFLGVS PHYNYSEALT FDSHKGFWCQ LLEEGKTKCL GKSKGRKYPP MDSDSRAFLS 840 SYYRDHNVEL SKLLHRLGQP LPSWLRQELQ KVR 873 |
Abbreviation :
CLO : cleft lip only. CPO : cleft palate only.
CLP : cleft lip and palate. CL/P : cleft lip with/without cleft palate.
For humans: CL/P, CLO, CPO, and CLP. For mice: CLO, CLP, and CPO.
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0008146 | sulfotransferase activity | IDA |
| GO:0015016 | [heparan sulfate]-glucosamine N-sulfotransferase activity | ISO |
| GO:0015016 | [heparan sulfate]-glucosamine N-sulfotransferase activity | IBA |
| GO:0019213 | deacetylase activity | IDA |
| GO:0019213 | deacetylase activity | IBA |
| GO:0042328 | heparan sulfate N-acetylglucosaminyltransferase activity | ISO |
| GO:0042328 | heparan sulfate N-acetylglucosaminyltransferase activity | IBA |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0015012 | heparan sulfate proteoglycan biosynthetic process | ISO |
| GO:0015014 | heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process | ISO |
| GO:0015014 | heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process | IBA |
| GO:0030210 | heparin biosynthetic process | IEA |
| GO ID | GO Term | Evidence |
|---|---|---|
| GO:0000139 | Golgi membrane | IEA |
| GO:0005794 | Golgi apparatus | IBA |
| GO:0016021 | integral component of membrane | IEA |
| Reactome ID | Reactome Term | Evidence |
|---|---|---|
| R-MMU-1430728 | Metabolism | IEA |
| R-MMU-1630316 | Glycosaminoglycan metabolism | IEA |
| R-MMU-1638091 | Heparan sulfate/heparin (HS-GAG) metabolism | IEA |
| R-MMU-2022928 | HS-GAG biosynthesis | IEA |
| R-MMU-71387 | Metabolism of carbohydrates | IEA |