ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Capn5

Full Name:

calpain 5

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

7 E2

Physical Location:

105270074-105326702 on NC_000073.5, complement

Gene Type:

protein-coding

Human Ortholog:

GeneID: 726 Symbol (Name): CAPN5 (calpain 5)

Ortholog Status:

The human GeneID 726 is not in current human dataset.

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		U85020
Fold Change:		2.5, 1.7
Note:		ILS preferentially expressed gene. Two ratios of ILS/ISS are from cDNA array and RT-PCR.

Dataset Information

Name:		11410715
Method:		Microarray
Tissue:		Whole brain
Phenotype:		Ethanol sensitivity
Publication:		Xu et al. Alcohol Clin Exp Res. (2001) Comparison of global brain gene expression profiles between inbred long-sleep and inbred short-sleep mice by high-density gene array hybridization. PubMed
Summary:		Inbred long-sleep (ILS) and short-sleep (ISS) mice show significant central nervous system-mediated differences in sleep time for sedative dose of ethanol and are frequently used as a rodent model for ethanol sensitivity. In this study, we used both the gene discovery array (GDA) and the Mouse GEM 1 Microarray. Complex probes were prepared from total brain mRNA of ILS or ISS mice by using reverse transcription and 33P labeling. Differentially expressed genes identified from each method were confirmed by relative quantitative reverse transcription-polymerase chain reaction (RT-PCR). A total of 41 genes or expressed sequence tags (ESTs) display significant expression level differences between brains of ILS and ISS mice after GDA, GEM1 hybridization, and quantitative RTPCR confirmation. Among them, 18 clones were expressed higher in ILS mice, and 23 clones were expressed higher in ISS mice.

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_007602.3	NP_031628.1	NC_000073.5 range: 105270074..105326702, complement

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0005622	intracellular	Cellular Component	IEA
GO:0004197	cysteine-type endopeptidase activity	Molecular Function	IEA
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	Molecular Function	IEA
GO:0008233	peptidase activity	Molecular Function	IEA
GO:0008234	cysteine-type peptidase activity	Molecular Function	IEA
GO:0016787	hydrolase activity	Molecular Function	IEA
GO:0006508	proteolysis	Biological Process	IEA