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Dataset Description
  • Dataset Name: 11410715
  • Species: Mouse
  • Method: Microarray
  • No. of Genes: 36
  • Tissue: Whole brain
  • Phenotype: Ethanol sensitivity
  • Publication: Xu et al. Alcohol Clin Exp Res. (2001) Comparison of global brain gene expression profiles between inbred long-sleep and inbred short-sleep mice by high-density gene array hybridization. PubMed
  • Dataset Summary: Inbred long-sleep (ILS) and short-sleep (ISS) mice show significant central nervous system-mediated differences in sleep time for sedative dose of ethanol and are frequently used as a rodent model for ethanol sensitivity. In this study, we used both the gene discovery array (GDA) and the Mouse GEM 1 Microarray. Complex probes were prepared from total brain mRNA of ILS or ISS mice by using reverse transcription and 33P labeling. Differentially expressed genes identified from each method were confirmed by relative quantitative reverse transcription-polymerase chain reaction (RT-PCR). A total of 41 genes or expressed sequence tags (ESTs) display significant expression level differences between brains of ILS and ISS mice after GDA, GEM1 hybridization, and quantitative RTPCR confirmation. Among them, 18 clones were expressed higher in ILS mice, and 23 clones were expressed higher in ISS mice.

    Gene list of this dataset (36 genes)
    GeneIDSymbolNameGenetic locationOriginal ID1Original ID2
    16763Lad1ladinin1 E4U58011 
    18016Nf2neurofibromatosis 211 A1|11 0.25 cML27090 
    16453Jak3Janus kinase 38 B3.3|8 33.0 cMQ62137 
    20190Ryr1ryanodine receptor 1, skeletal muscle7 A2-B3|7 10.0 cMU23754 
    12337Capn5calpain 57 E2U85020 
    52715Ccdc43coiled-coil domain containing 4311 E1|11 60.0 cMW97942 
    13043Cttncortactin7 F5U03184 
    77106Tmem181transmembrane protein 18117 A1AA423204 
    673924833420G17RikRIKEN cDNA 4833420G17 gene13 D2.3W84055 
    3812806430706D22RikRIKEN cDNA 6430706D22 gene1 DAA048171 
    71472Usp19ubiquitin specific peptidase 199 F2AA254871 
    69908Rab3bRAB3B, member RAS oncogene family4 C7AA030574 
    18115Nntnicotinamide nucleotide transhydrogenase13 D2|13 64.0 cMAF037327 
    218232Ptpdc1protein tyrosine phosphatase domain containing 113 A5AA388221 
    67760Slc38a2solute carrier family 38, member 215 F1AA245554 
    760005033430I15RikRIKEN cDNA 5033430I15 gene13 A5AA221680 
    54371Chst2carbohydrate sulfotransferase 2-AB011451 
    27999D6Wsu176eDNA segment, Chr 6, Wayne State University 176, expressed6 A3.1|6 2.0 cMAA509602 
    319839A530020G20RikRIKEN cDNA A530020G20 gene3 G1AA543468 
    23831Car14carbonic anhydrase 143 F2.1AB005450 
    730742900086B20RikRIKEN cDNA 2900086B20 gene-AA060336 
    12709Ckbcreatine kinase, brain12 F1|12 55.0 cMM74149 
    234407Glt25d1glycosyltransferase 25 domain containing 18 B3.3W36470 
    18597Pdha1pyruvate dehydrogenase E1 alpha 1X F3-F4|X 66.5 cMM76727 
    22709Zfp51zinc finger protein 5117 A3.2|17 9.9 cMM74235 
    19063Ppt1palmitoyl-protein thioesterase 14 D1-D3|4 59.1 cMAF071025 
    15018H2-Q7histocompatibility 2, Q region locus 717 B1|17 19.19 cMX05389 
    319944Taf2TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor15 D1AA028778 
    76987Hdhd2haloacid dehalogenase-like hydrolase domain containing 218 E3AA268225 
    15018H2-Q7histocompatibility 2, Q region locus 717 B1|17 19.19 cMX03210 
    170942Erdr1erythroid differentiation regulator 1-AJ007909