ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Nnt

Full Name:

nicotinamide nucleotide transhydrogenase

Alias:

4930423F13Rik|AI323702|BB168308

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

13 D2|13 64.0 cM

Physical Location:

120123123-120197817 on NC_000079.5, complement

Gene Type:

protein-coding

Human Ortholog:

GeneID: 23530 Symbol (Name): NNT (nicotinamide nucleotide transhydrogenase)

Ortholog Status:

The human GeneID 23530 is also in human dataset(s).

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		AF037327
Fold Change:		1.7, 1.7
Note:		ILS preferentially expressed gene. Two ratios of ILS/ISS are from cDNA array and RT-PCR.

Dataset Information

Name:		11410715
Method:		Microarray
Tissue:		Whole brain
Phenotype:		Ethanol sensitivity
Publication:		Xu et al. Alcohol Clin Exp Res. (2001) Comparison of global brain gene expression profiles between inbred long-sleep and inbred short-sleep mice by high-density gene array hybridization. PubMed
Summary:		Inbred long-sleep (ILS) and short-sleep (ISS) mice show significant central nervous system-mediated differences in sleep time for sedative dose of ethanol and are frequently used as a rodent model for ethanol sensitivity. In this study, we used both the gene discovery array (GDA) and the Mouse GEM 1 Microarray. Complex probes were prepared from total brain mRNA of ILS or ISS mice by using reverse transcription and 33P labeling. Differentially expressed genes identified from each method were confirmed by relative quantitative reverse transcription-polymerase chain reaction (RT-PCR). A total of 41 genes or expressed sequence tags (ESTs) display significant expression level differences between brains of ILS and ISS mice after GDA, GEM1 hybridization, and quantitative RTPCR confirmation. Among them, 18 clones were expressed higher in ILS mice, and 23 clones were expressed higher in ISS mice.

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_008710.3	NP_032736.2	NC_000079.5 range: 120123123..120197817, complement

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0016021	integral to membrane	Cellular Component	RCA (12466851)
GO:0005739	mitochondrion	Cellular Component	IDA (14651853)
GO:0005743	mitochondrial inner membrane	Cellular Component	IDA (12865426)
GO:0016020	membrane	Cellular Component	IEA
GO:0003824	catalytic activity	Molecular Function	IEA
GO:0009055	electron carrier activity	Molecular Function	IEA
GO:0008750	NAD(P)+ transhydrogenase (AB-specific) activity	Molecular Function	IEA
GO:0008746	NAD(P) transhydrogenase activity	Molecular Function	IEA
GO:0005488	binding	Molecular Function	IEA
GO:0016491	oxidoreductase activity	Molecular Function	IEA
GO:0015992	proton transport	Biological Process	IEA
GO:0008152	metabolic process	Biological Process	IEA