| Gene Information | | Original ID1: | | AA060336 | | Fold Change: | | 2.8, 3.3 | | Note: | | ISS preferentially expressed gene. Two ratios of ISS/ILS are from cDNA array and RT-PCR. |
| | Dataset Information | | Name: | | 11410715 | | Method: | | Microarray | | Tissue: | | Whole brain | | Phenotype: | | Ethanol sensitivity | | Publication: | | Xu et al. Alcohol Clin Exp Res. (2001) Comparison of global brain gene expression profiles between inbred long-sleep and inbred short-sleep mice by high-density gene array hybridization. PubMed | | Summary: | | Inbred long-sleep (ILS) and short-sleep (ISS) mice show significant central nervous system-mediated differences in sleep time for sedative dose of ethanol and are frequently used as a rodent model for ethanol sensitivity. In this study, we used both the gene discovery array (GDA) and the Mouse GEM 1 Microarray. Complex probes were prepared from total brain mRNA of ILS or ISS mice by using reverse transcription and 33P labeling. Differentially expressed genes identified from each method were confirmed by relative quantitative reverse transcription-polymerase chain reaction (RT-PCR). A total of 41 genes or expressed sequence tags (ESTs) display significant expression level differences between brains of ILS and ISS mice after GDA, GEM1 hybridization, and quantitative RTPCR confirmation. Among them, 18 clones were expressed higher in ILS mice, and 23 clones were expressed higher in ISS mice. |
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