ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

18016

Symbol:

Nf2

Full Name:

neurofibromatosis 2

Alias:

merlin

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

11 A1|11 0.25 cM

Physical Location:

4665850-4749529 on NC_000077.5, complement

Gene Type:

protein-coding

Human Ortholog:

GeneID: 4771 Symbol (Name): NF2 (neurofibromin 2 (merlin))

Ortholog Status:

The human GeneID 4771 is also in human dataset(s).

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		L27090
Fold Change:		4.5, 3.6
Note:		ILS preferentially expressed gene. Two ratios of ILS/ISS are from cDNA array and RT-PCR.

Dataset Information

Name:		11410715
Method:		Microarray
Tissue:		Whole brain
Phenotype:		Ethanol sensitivity
Publication:		Xu et al. Alcohol Clin Exp Res. (2001) Comparison of global brain gene expression profiles between inbred long-sleep and inbred short-sleep mice by high-density gene array hybridization. PubMed
Summary:		Inbred long-sleep (ILS) and short-sleep (ISS) mice show significant central nervous system-mediated differences in sleep time for sedative dose of ethanol and are frequently used as a rodent model for ethanol sensitivity. In this study, we used both the gene discovery array (GDA) and the Mouse GEM 1 Microarray. Complex probes were prepared from total brain mRNA of ILS or ISS mice by using reverse transcription and 33P labeling. Differentially expressed genes identified from each method were confirmed by relative quantitative reverse transcription-polymerase chain reaction (RT-PCR). A total of 41 genes or expressed sequence tags (ESTs) display significant expression level differences between brains of ILS and ISS mice after GDA, GEM1 hybridization, and quantitative RTPCR confirmation. Among them, 18 clones were expressed higher in ILS mice, and 23 clones were expressed higher in ISS mice.

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_010898.3	NP_035028.2	NC_000077.5 range: 4665850..4749529, complement

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0030175	filopodium	Cellular Component	IDA (9563848)
GO:0032154	cleavage furrow	Cellular Component	IDA (9631655)
GO:0019898	extrinsic to membrane	Cellular Component	IEA
GO:0030027	lamellipodium	Cellular Component	IDA (9563848)
GO:0005912	adherens junction	Cellular Component	IMP (12695331)
GO:0005856	cytoskeleton	Cellular Component	IEA
GO:0005737	cytoplasm	Cellular Component	IDA (8547603\|15133494)
GO:0005634	nucleus	Cellular Component	IDA (15133494)
GO:0001726	ruffle	Cellular Component	IDA (9563848)
GO:0005515	protein binding	Molecular Function	IPI (12695331\|14580336\|16405865)
GO:0005488	binding	Molecular Function	IEA
GO:0005198	structural molecule activity	Molecular Function	IEA
GO:0008092	cytoskeletal protein binding	Molecular Function	IEA
GO:0042127	regulation of cell proliferation	Biological Process	IMP (12695331)
GO:0042475	odontogenesis of dentine-containing teeth	Biological Process	IMP (10887156)
GO:0043409	negative regulation of MAPKKK cascade	Biological Process	IMP (16405865)
GO:0045216	cell-cell junction assembly and maintenance	Biological Process	IMP (12695331)
GO:0001707	mesoderm formation	Biological Process	IMP (9171370)
GO:0008285	negative regulation of cell proliferation	Biological Process	IMP (9553042\|10887156)
GO:0007398	ectoderm development	Biological Process	IMP (9171370)
GO:0007049	cell cycle	Biological Process	IEA
GO:0006469	negative regulation of protein kinase activity	Biological Process	IMP (16405865)
GO:0006469	negative regulation of protein kinase activity	Biological Process	IDA (14580336)
GO:0045786	negative regulation of cell cycle	Biological Process	IEA