ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Ckb

Full Name:

creatine kinase, brain

Alias:

B-CK|Ck-3

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

12 F1|12 55.0 cM

Physical Location:

112907571-112910478 on NC_000078.5, complement

Gene Type:

protein-coding

Human Ortholog:

GeneID: 1152 Symbol (Name): CKB (creatine kinase, brain)

Ortholog Status:

The human GeneID 1152 is not in current human dataset.

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		M74149
Fold Change:		2.5, 1.8
Note:		ISS preferentially expressed gene. Two ratios of ISS/ILS are from cDNA array and RT-PCR.

Dataset Information

Name:		11410715
Method:		Microarray
Tissue:		Whole brain
Phenotype:		Ethanol sensitivity
Publication:		Xu et al. Alcohol Clin Exp Res. (2001) Comparison of global brain gene expression profiles between inbred long-sleep and inbred short-sleep mice by high-density gene array hybridization. PubMed
Summary:		Inbred long-sleep (ILS) and short-sleep (ISS) mice show significant central nervous system-mediated differences in sleep time for sedative dose of ethanol and are frequently used as a rodent model for ethanol sensitivity. In this study, we used both the gene discovery array (GDA) and the Mouse GEM 1 Microarray. Complex probes were prepared from total brain mRNA of ILS or ISS mice by using reverse transcription and 33P labeling. Differentially expressed genes identified from each method were confirmed by relative quantitative reverse transcription-polymerase chain reaction (RT-PCR). A total of 41 genes or expressed sequence tags (ESTs) display significant expression level differences between brains of ILS and ISS mice after GDA, GEM1 hybridization, and quantitative RTPCR confirmation. Among them, 18 clones were expressed higher in ILS mice, and 23 clones were expressed higher in ISS mice.

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_021273.3	NP_067248.1	NC_000078.5 range: 112907571..112910478, complement

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0005737	cytoplasm	Cellular Component	IEA
GO:0005739	mitochondrion	Cellular Component	IDA (14651853)
GO:0000166	nucleotide binding	Molecular Function	IEA
GO:0003824	catalytic activity	Molecular Function	IEA
GO:0004111	creatine kinase activity	Molecular Function	IEA
GO:0005524	ATP binding	Molecular Function	IEA
GO:0016301	kinase activity	Molecular Function	IEA
GO:0016740	transferase activity	Molecular Function	IEA
GO:0016772	transferase activity, transferring phosphorus-containing groups	Molecular Function	IEA