ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Jak3

Full Name:

Janus kinase 3

Alias:

fae|wil

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

8 B3.3|8 33.0 cM

Physical Location:

74200472-74214472 on NC_000074.5

Gene Type:

protein-coding

Human Ortholog:

GeneID: 3718 Symbol (Name): JAK3 (Janus kinase 3 (a protein tyrosine kinase, leukocyte))

Ortholog Status:

The human GeneID 3718 is also in human dataset(s).

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		Q62137
Fold Change:		4.0, 2.2
Note:		ILS preferentially expressed gene. Two ratios of ILS/ISS are from cDNA array and RT-PCR.

Dataset Information

Name:		11410715
Method:		Microarray
Tissue:		Whole brain
Phenotype:		Ethanol sensitivity
Publication:		Xu et al. Alcohol Clin Exp Res. (2001) Comparison of global brain gene expression profiles between inbred long-sleep and inbred short-sleep mice by high-density gene array hybridization. PubMed
Summary:		Inbred long-sleep (ILS) and short-sleep (ISS) mice show significant central nervous system-mediated differences in sleep time for sedative dose of ethanol and are frequently used as a rodent model for ethanol sensitivity. In this study, we used both the gene discovery array (GDA) and the Mouse GEM 1 Microarray. Complex probes were prepared from total brain mRNA of ILS or ISS mice by using reverse transcription and 33P labeling. Differentially expressed genes identified from each method were confirmed by relative quantitative reverse transcription-polymerase chain reaction (RT-PCR). A total of 41 genes or expressed sequence tags (ESTs) display significant expression level differences between brains of ILS and ISS mice after GDA, GEM1 hybridization, and quantitative RTPCR confirmation. Among them, 18 clones were expressed higher in ILS mice, and 23 clones were expressed higher in ISS mice.

Gene Information

Original ID1:		Jak3
Original ID2:		AI854103

Dataset Information

Name:		15745951
Method:		Microarray
Tissue:		Nucleus accumbens, prefrontal cortex, and ventral tegmental area
Phenotype:		Acute ethanol response
Publication:		Kerns et al. J Neurosci. (2005) Ethanol-responsive brain region expression networks: implications for behavioral responses to acute ethanol in DBA/2J versus C57BL/6J mice. PubMed
Summary:		The inbred mouse strains DBA/2J and C57BL/6J exhibit contrasting acute behavioral responses to ethanol. We used oligonucleotide microarrays and bioinformatics methods to characterize patterns of gene expression in three brain regions of the mesolimbic reward pathway of these strains. Expression profiling included examination of both differences in gene expression 4 h after acute ethanol (2 g/kg). Using a rigorous stepwise method for microarray analysis, we identified 307 ethanol-regulated genes in the nucleus accumbens, prefrontal cortex, and ventral tegmental area.

QTL Information

QTL	Phenotype	Chr	Peak (cM)	LOD	P Value	Range	Status	QTL Link
Caws3/Alcw6	Chronic Alcohol Withdrawal	8		16-43 (cM)	3.5	0.0003	Significant	PARC

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_010589.5	NP_034719.2	NC_000074.5 range: 74200472..74214472

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0005856	cytoskeleton	Cellular Component	IEA
GO:0016020	membrane	Cellular Component	IEA
GO:0016301	kinase activity	Molecular Function	IEA
GO:0000166	nucleotide binding	Molecular Function	IEA
GO:0005524	ATP binding	Molecular Function	IEA
GO:0005515	protein binding	Molecular Function	IPI (10872802)
GO:0004718	Janus kinase activity	Molecular Function	IDA (8022486)
GO:0004715	non-membrane spanning protein tyrosine kinase activity	Molecular Function	IEA
GO:0004713	protein-tyrosine kinase activity	Molecular Function	IDA (10872802)
GO:0004672	protein kinase activity	Molecular Function	IEA
GO:0016740	transferase activity	Molecular Function	IEA
GO:0045944	positive regulation of transcription from RNA polymerase II promoter	Biological Process	IMP (10485657)
GO:0019221	cytokine and chemokine mediated signaling pathway	Biological Process	IDA (8022486)
GO:0018108	peptidyl-tyrosine phosphorylation	Biological Process	IDA (10872802)
GO:0007243	protein kinase cascade	Biological Process	IDA (8022486)
GO:0007167	enzyme linked receptor protein signaling pathway	Biological Process	IDA (8022486)
GO:0006468	protein amino acid phosphorylation	Biological Process	IEA
GO:0048535	lymph node development	Biological Process	TAS (10687308)