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Gene Information
Gene ID:69908
Full Name:RAB3B, member RAS oncogene family
Organism:Mus musculus (Mouse)
Genetic Location:4 C7
Physical Location:108551674-108615928 on NC_000070.5
Gene Type:protein-coding
Human Ortholog:GeneID: 5865    Symbol (Name): RAB3B (RAB3B, member RAS oncogene family)
Ortholog Status:The human GeneID 5865 is also in human dataset(s).
Gene in Ethanol Study Datasets
Gene Information
Original ID1:AA030574
Fold Change:1.8, 1.5
Note:ILS preferentially expressed gene. Two ratios of ILS/ISS are from cDNA array and RT-PCR.
Dataset Information
Tissue:Whole brain
Phenotype:Ethanol sensitivity
Publication:Xu et al. Alcohol Clin Exp Res. (2001) Comparison of global brain gene expression profiles between inbred long-sleep and inbred short-sleep mice by high-density gene array hybridization. PubMed
Summary:Inbred long-sleep (ILS) and short-sleep (ISS) mice show significant central nervous system-mediated differences in sleep time for sedative dose of ethanol and are frequently used as a rodent model for ethanol sensitivity. In this study, we used both the gene discovery array (GDA) and the Mouse GEM 1 Microarray. Complex probes were prepared from total brain mRNA of ILS or ISS mice by using reverse transcription and 33P labeling. Differentially expressed genes identified from each method were confirmed by relative quantitative reverse transcription-polymerase chain reaction (RT-PCR). A total of 41 genes or expressed sequence tags (ESTs) display significant expression level differences between brains of ILS and ISS mice after GDA, GEM1 hybridization, and quantitative RTPCR confirmation. Among them, 18 clones were expressed higher in ILS mice, and 23 clones were expressed higher in ISS mice.
Gene Information
Original ID1:Rab3b
Fold Change:0.72
Note:Significantly regulated by ethanol in cortical neurons
Gene Information
Original ID1:Rab3b
Fold Change:0.69
Note:Significantly regulated in cortical neurons treated with both ethanol and nicotine
Dataset Information
Tissue:Cultured fetal mouse cortical neurons
Phenotype:Ethanol response
Publication:Wang et al. Alcohol Clin Exp Res. (2007) Regulation of platelet-derived growth factor signaling pathway by ethanol, nicotine, or both in mouse cortical neurons. PubMed
Summary:By using cultured fetal mouse cortical neurons as a model system, we sought to identify genes and pathways that are modulated in the cells by ethanol, nicotine, or both. METHODS: Primary cerebral cortical cultures were prepared from the brains of 14-day-old C57BL/6 mouse fetuses and exposed to ethanol (75 mM), nicotine (0.1 mM), or both for 5 consecutive days. A homeostatic pathway-focused microarray consisting of 638 sequence-verified genes was used to measure transcripts differentially regulated by ethanol, nicotine, or both in 5 drug-treated cortical neuron samples and 5 control samples. We identified 65, 111, and 81 significantly regulated genes in the ethanol, nicotine, and ethanol/nicotine-treated neurons, respectively. A gene was considered to be significantly regulated if (1) the fold change was 20% greater or less compared with the controls and (2) the difference was p<0.05 by Student's t-test.
Gene Refseq Sequence Annotation
mRNAProteinReference assembly Genomic
NM_023537.5NP_076026.1NC_000070.5 range: 108551674..108615928
Gene Ontology (GO) Annotation
GO IDGO TermCategoryEvidence (PubMed)
GO:0030141secretory granuleCellular ComponentIDA (16043482)
GO:0016020membraneCellular ComponentIEA
GO:0008021synaptic vesicleCellular ComponentIDA (12167638|12192047)
GO:0005525GTP bindingMolecular FunctionIEA
GO:0003924GTPase activityMolecular FunctionRCA (12466851)
GO:0008565protein transporter activityMolecular FunctionRCA (12466851)
GO:0005515protein bindingMolecular FunctionIPI (12578829|15159548)
GO:0000166nucleotide bindingMolecular FunctionIEA
GO:0007264small GTPase mediated signal transductionBiological ProcessRCA (12466851)
GO:0006886intracellular protein transportBiological ProcessRCA (12466851)
GO:0015031protein transportBiological ProcessRCA (12466851)
GO:0006810transportBiological ProcessIEA
GO:0017157regulation of exocytosisBiological ProcessIDA (12167638)
GO:0018125peptidyl-cysteine methylationBiological ProcessIDA (11121396)
Other Database Cross Links
NCBI Entrez Gene:69908