ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Gapdh

Full Name:

glyceraldehyde-3-phosphate dehydrogenase

Alias:

Gapd|MGC102544|MGC102546|MGC103190|MGC103191|MGC105239

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

6 F2

Physical Location:

125111869-125115600 on NC_000072.5, complement

Gene Type:

protein-coding

Human Ortholog:

GeneID: 2597 Symbol (Name): GAPDH (glyceraldehyde-3-phosphate dehydrogenase)

Ortholog Status:

The human GeneID 2597 is also in human dataset(s).

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		Gapd
Fold Change:		0.69
Note:		Significantly regulated in cortical neurons treated with both ethanol and nicotine

Dataset Information

Name:		17295719
Method:		Microarray
Tissue:		Cultured fetal mouse cortical neurons
Phenotype:		Ethanol response
Publication:		Wang et al. Alcohol Clin Exp Res. (2007) Regulation of platelet-derived growth factor signaling pathway by ethanol, nicotine, or both in mouse cortical neurons. PubMed
Summary:		By using cultured fetal mouse cortical neurons as a model system, we sought to identify genes and pathways that are modulated in the cells by ethanol, nicotine, or both. METHODS: Primary cerebral cortical cultures were prepared from the brains of 14-day-old C57BL/6 mouse fetuses and exposed to ethanol (75 mM), nicotine (0.1 mM), or both for 5 consecutive days. A homeostatic pathway-focused microarray consisting of 638 sequence-verified genes was used to measure transcripts differentially regulated by ethanol, nicotine, or both in 5 drug-treated cortical neuron samples and 5 control samples. We identified 65, 111, and 81 significantly regulated genes in the ethanol, nicotine, and ethanol/nicotine-treated neurons, respectively. A gene was considered to be significantly regulated if (1) the fold change was 20% greater or less compared with the controls and (2) the difference was p<0.05 by Student's t-test.

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_008084.2	NP_032110.1	NC_000072.5 range: 125111869..125115600, complement

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0005737	cytoplasm	Cellular Component	IDA (15159393)
GO:0005739	mitochondrion	Cellular Component	IDA (14651853)
GO:0004365	glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity	Molecular Function	IEA
GO:0005515	protein binding	Molecular Function	IPI (17500595)
GO:0008943	glyceraldehyde-3-phosphate dehydrogenase activity	Molecular Function	IEA
GO:0016491	oxidoreductase activity	Molecular Function	IEA
GO:0051287	NAD binding	Molecular Function	IEA
GO:0006006	glucose metabolic process	Biological Process	IEA
GO:0006096	glycolysis	Biological Process	IEA