ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Jun

Full Name:

Jun oncogene

Alias:

AP-1|Junc|c-jun

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

4 C5-C7|4 44.6 cM

Physical Location:

94715745-94718877 on NC_000070.5, complement

Gene Type:

protein-coding

Human Ortholog:

GeneID: 3725 Symbol (Name): JUN (jun oncogene)

Ortholog Status:

The human GeneID 3725 is also in human dataset(s).

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		Jun
Original ID2:		X12761

Dataset Information

Name:		15745951
Method:		Microarray
Tissue:		Nucleus accumbens, prefrontal cortex, and ventral tegmental area
Phenotype:		Acute ethanol response
Publication:		Kerns et al. J Neurosci. (2005) Ethanol-responsive brain region expression networks: implications for behavioral responses to acute ethanol in DBA/2J versus C57BL/6J mice. PubMed
Summary:		The inbred mouse strains DBA/2J and C57BL/6J exhibit contrasting acute behavioral responses to ethanol. We used oligonucleotide microarrays and bioinformatics methods to characterize patterns of gene expression in three brain regions of the mesolimbic reward pathway of these strains. Expression profiling included examination of both differences in gene expression 4 h after acute ethanol (2 g/kg). Using a rigorous stepwise method for microarray analysis, we identified 307 ethanol-regulated genes in the nucleus accumbens, prefrontal cortex, and ventral tegmental area.

Gene Information

Original ID1:		Jun
Fold Change:		0.76
Note:		Significantly regulated in cortical neurons treated with both ethanol and nicotine

Dataset Information

Name:		17295719
Method:		Microarray
Tissue:		Cultured fetal mouse cortical neurons
Phenotype:		Ethanol response
Publication:		Wang et al. Alcohol Clin Exp Res. (2007) Regulation of platelet-derived growth factor signaling pathway by ethanol, nicotine, or both in mouse cortical neurons. PubMed
Summary:		By using cultured fetal mouse cortical neurons as a model system, we sought to identify genes and pathways that are modulated in the cells by ethanol, nicotine, or both. METHODS: Primary cerebral cortical cultures were prepared from the brains of 14-day-old C57BL/6 mouse fetuses and exposed to ethanol (75 mM), nicotine (0.1 mM), or both for 5 consecutive days. A homeostatic pathway-focused microarray consisting of 638 sequence-verified genes was used to measure transcripts differentially regulated by ethanol, nicotine, or both in 5 drug-treated cortical neuron samples and 5 control samples. We identified 65, 111, and 81 significantly regulated genes in the ethanol, nicotine, and ethanol/nicotine-treated neurons, respectively. A gene was considered to be significantly regulated if (1) the fold change was 20% greater or less compared with the controls and (2) the difference was p<0.05 by Student's t-test.

QTL Information

QTL	Phenotype	Chr	Peak (cM)	LOD	P Value	Range	Status	QTL Link
Caws2	Chronic Alcohol Withdrawal	4		33-51 (cM)	2.3	0.005	Significant	PARC

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_010591.2	NP_034721.1	NC_000070.5 range: 94715745..94718877, complement

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0005634	nucleus	Cellular Component	IDA (12050152)
GO:0005667	transcription factor complex	Cellular Component	IDA (12798298\|12943993)
GO:0003677	DNA binding	Molecular Function	IDA (10076871\|12798298)
GO:0003700	transcription factor activity	Molecular Function	IEA
GO:0005515	protein binding	Molecular Function	IPI (12798298)
GO:0046983	protein dimerization activity	Molecular Function	IEA
GO:0043565	sequence-specific DNA binding	Molecular Function	IEA
GO:0045944	positive regulation of transcription from RNA polymerase II promoter	Biological Process	IDA (12798298)
GO:0042493	response to drug	Biological Process	IDA (9098922)
GO:0035026	leading edge cell differentiation	Biological Process	IMP (12791271)
GO:0031953	negative regulation of protein amino acid autophosphorylation	Biological Process	IMP (16916642)
GO:0009987	cellular process	Biological Process	IMP (11818961)
GO:0006355	regulation of transcription, DNA-dependent	Biological Process	IMP (11818961)
GO:0006350	transcription	Biological Process	IEA
GO:0051726	regulation of cell cycle	Biological Process	IMP (11818961)