ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Map3k3

Full Name:

mitogen-activated protein kinase kinase kinase 3

Alias:

AW548911|KIAA4031|MAPKKK3|Mekk3|mKIAA4031

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

11 E1

Physical Location:

105946215-106016759 on NC_000077.5

Gene Type:

protein-coding

Human Ortholog:

GeneID: 4215 Symbol (Name): MAP3K3 (mitogen-activated protein kinase kinase kinase 3)

Ortholog Status:

The human GeneID 4215 is also in human dataset(s).

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		Map3k3
Fold Change:		0.63
Note:		Significantly regulated by ethanol in cortical neurons

Dataset Information

Name:		17295719
Method:		Microarray
Tissue:		Cultured fetal mouse cortical neurons
Phenotype:		Ethanol response
Publication:		Wang et al. Alcohol Clin Exp Res. (2007) Regulation of platelet-derived growth factor signaling pathway by ethanol, nicotine, or both in mouse cortical neurons. PubMed
Summary:		By using cultured fetal mouse cortical neurons as a model system, we sought to identify genes and pathways that are modulated in the cells by ethanol, nicotine, or both. METHODS: Primary cerebral cortical cultures were prepared from the brains of 14-day-old C57BL/6 mouse fetuses and exposed to ethanol (75 mM), nicotine (0.1 mM), or both for 5 consecutive days. A homeostatic pathway-focused microarray consisting of 638 sequence-verified genes was used to measure transcripts differentially regulated by ethanol, nicotine, or both in 5 drug-treated cortical neuron samples and 5 control samples. We identified 65, 111, and 81 significantly regulated genes in the ethanol, nicotine, and ethanol/nicotine-treated neurons, respectively. A gene was considered to be significantly regulated if (1) the fold change was 20% greater or less compared with the controls and (2) the difference was p<0.05 by Student's t-test.

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_011947.3	NP_036077.1	NC_000077.5 range: 105946215..106016759

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0000166	nucleotide binding	Molecular Function	IEA
GO:0016740	transferase activity	Molecular Function	IEA
GO:0016301	kinase activity	Molecular Function	IEA
GO:0005524	ATP binding	Molecular Function	IEA
GO:0005515	protein binding	Molecular Function	IPI (12912994\|14661019)
GO:0004709	MAP kinase kinase kinase activity	Molecular Function	IEA
GO:0004674	protein serine/threonine kinase activity	Molecular Function	IEA
GO:0004672	protein kinase activity	Molecular Function	ISS (15001576)
GO:0000287	magnesium ion binding	Molecular Function	IEA
GO:0046872	metal ion binding	Molecular Function	IEA
GO:0006468	protein amino acid phosphorylation	Biological Process	IEA
GO:0007243	protein kinase cascade	Biological Process	ISS (15001576)
GO:0046777	protein amino acid autophosphorylation	Biological Process	ISS (15001576)