ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Psma4

Full Name:

proteasome (prosome, macropain) subunit, alpha type 4

Alias:

Organism:

Mus musculus (Mouse)

Chromosome:

Genetic Location:

9 C

Physical Location:

54798665-54805836 on NC_000075.5

Gene Type:

protein-coding

Human Ortholog:

GeneID: 5685 Symbol (Name): PSMA4 (proteasome (prosome, macropain) subunit, alpha type, 4)

Ortholog Status:

The human GeneID 5685 is not in current human dataset.

Gene in Ethanol Study Datasets

Gene Information

Original ID1:		Psma4
Original ID2:		AV266216

Dataset Information

Name:		15745951
Method:		Microarray
Tissue:		Nucleus accumbens, prefrontal cortex, and ventral tegmental area
Phenotype:		Acute ethanol response
Publication:		Kerns et al. J Neurosci. (2005) Ethanol-responsive brain region expression networks: implications for behavioral responses to acute ethanol in DBA/2J versus C57BL/6J mice. PubMed
Summary:		The inbred mouse strains DBA/2J and C57BL/6J exhibit contrasting acute behavioral responses to ethanol. We used oligonucleotide microarrays and bioinformatics methods to characterize patterns of gene expression in three brain regions of the mesolimbic reward pathway of these strains. Expression profiling included examination of both differences in gene expression 4 h after acute ethanol (2 g/kg). Using a rigorous stepwise method for microarray analysis, we identified 307 ethanol-regulated genes in the nucleus accumbens, prefrontal cortex, and ventral tegmental area.

Gene Information

Original ID1:		Psma4
Fold Change:		1.32
Note:		Significantly regulated in cortical neurons treated with both ethanol and nicotine

Dataset Information

Name:		17295719
Method:		Microarray
Tissue:		Cultured fetal mouse cortical neurons
Phenotype:		Ethanol response
Publication:		Wang et al. Alcohol Clin Exp Res. (2007) Regulation of platelet-derived growth factor signaling pathway by ethanol, nicotine, or both in mouse cortical neurons. PubMed
Summary:		By using cultured fetal mouse cortical neurons as a model system, we sought to identify genes and pathways that are modulated in the cells by ethanol, nicotine, or both. METHODS: Primary cerebral cortical cultures were prepared from the brains of 14-day-old C57BL/6 mouse fetuses and exposed to ethanol (75 mM), nicotine (0.1 mM), or both for 5 consecutive days. A homeostatic pathway-focused microarray consisting of 638 sequence-verified genes was used to measure transcripts differentially regulated by ethanol, nicotine, or both in 5 drug-treated cortical neuron samples and 5 control samples. We identified 65, 111, and 81 significantly regulated genes in the ethanol, nicotine, and ethanol/nicotine-treated neurons, respectively. A gene was considered to be significantly regulated if (1) the fold change was 20% greater or less compared with the controls and (2) the difference was p<0.05 by Student's t-test.

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_011966.3	NP_036096.1	NC_000075.5 range: 54798665..54805836

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0043234	protein complex	Cellular Component	IEA
GO:0005839	proteasome core complex	Cellular Component	IEA
GO:0005829	cytosol	Cellular Component	IEA
GO:0005737	cytoplasm	Cellular Component	IEA
GO:0005634	nucleus	Cellular Component	IEA
GO:0004298	threonine endopeptidase activity	Molecular Function	IEA
GO:0008233	peptidase activity	Molecular Function	IEA
GO:0016787	hydrolase activity	Molecular Function	IEA
GO:0004175	endopeptidase activity	Molecular Function	IEA
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IEA