ERGR - Ethanol-Related Gene Resource

Gene Information

Gene ID:

Symbol:

Map4k4

Full Name:

mitogen-activated protein kinase kinase kinase kinase 4

Alias:

9430080K19Rik|AU043147|AU045934|AW046177|HGK|Nik

Organism:

Mus musculus (Mouse)

Chromosome:

Physical Location:

39958074-40081769 on NC_000067.5

Gene Type:

protein-coding

Human Ortholog:

GeneID: 9448 Symbol (Name): MAP4K4 (mitogen-activated protein kinase kinase kinase kinase 4)

Ortholog Status:

The human GeneID 9448 is also in human dataset(s).

Gene in Ethanol Study Datasets

Gene Information

Original ID1:

Map4k4

Dataset Information

Name:		16618939
Method:		Microarray
Tissue:		Whole brain
Phenotype:		Alcohol preference
Publication:		Mulligan et al. Proc Natl Acad Sci U S A. (2006) Toward understanding the genetics of alcohol drinking through transcriptome meta-analysis. PubMed
Summary:		Microarray analyses of brain gene expression in three selected lines, and six isogenic strains of mice known to differ markedly in voluntary alcohol consumption provided >4.5 million data points for a meta-analysis. A total of 107 arrays were obtained and arranged into six experimental data sets, allowing the identification of 3,800 unique genes significantly and consistently changed between all models of high or low amounts of alcohol consumption. Here we select the 75 genes with the largest effect size (\|d\|) and lowest Q values.

Gene Information

Original ID1:		Map4k4
Fold Change:		1.54
Note:		Significantly regulated in cortical neurons treated with both ethanol and nicotine

Dataset Information

Name:		17295719
Method:		Microarray
Tissue:		Cultured fetal mouse cortical neurons
Phenotype:		Ethanol response
Publication:		Wang et al. Alcohol Clin Exp Res. (2007) Regulation of platelet-derived growth factor signaling pathway by ethanol, nicotine, or both in mouse cortical neurons. PubMed
Summary:		By using cultured fetal mouse cortical neurons as a model system, we sought to identify genes and pathways that are modulated in the cells by ethanol, nicotine, or both. METHODS: Primary cerebral cortical cultures were prepared from the brains of 14-day-old C57BL/6 mouse fetuses and exposed to ethanol (75 mM), nicotine (0.1 mM), or both for 5 consecutive days. A homeostatic pathway-focused microarray consisting of 638 sequence-verified genes was used to measure transcripts differentially regulated by ethanol, nicotine, or both in 5 drug-treated cortical neuron samples and 5 control samples. We identified 65, 111, and 81 significantly regulated genes in the ethanol, nicotine, and ethanol/nicotine-treated neurons, respectively. A gene was considered to be significantly regulated if (1) the fold change was 20% greater or less compared with the controls and (2) the difference was p<0.05 by Student's t-test.

Gene Refseq Sequence Annotation

mRNA	Protein	Reference assembly Genomic
NM_008696.2	NP_032722.2	NC_000067.5 range: 39958074..40081769

Gene Ontology (GO) Annotation

GO ID	GO Term	Category	Evidence (PubMed)
GO:0000166	nucleotide binding	Molecular Function	IEA
GO:0016301	kinase activity	Molecular Function	IEA
GO:0016740	transferase activity	Molecular Function	IEA
GO:0005524	ATP binding	Molecular Function	IDA (9135144)
GO:0005083	small GTPase regulator activity	Molecular Function	IEA
GO:0004674	protein serine/threonine kinase activity	Molecular Function	IDA (9135144)
GO:0004672	protein kinase activity	Molecular Function	IEA
GO:0006950	response to stress	Biological Process	IDA (9135144)
GO:0007243	protein kinase cascade	Biological Process	IDA (9135144)
GO:0006468	protein amino acid phosphorylation	Biological Process	IDA (9135144)