| Gene Information |
| Gene ID: | | 34291 |
| Symbol: | | Cyp4e3 |
| Alias: | | 4e3|CG4105|P-450|P450 |
| Organism: | | Drosophila melanogaster (Fly) |
| Chromosome: | | 2L |
| Genetic Location: | | 30C7-30C7 |
| Physical Location: | | 9748011-9750019 on NT_033779.4, complement |
| Gene Type: | | protein-coding |
| Human Ortholog: | | This gene has no human ortholog. |
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| Gene in Ethanol Study Datasets |
| Gene Information | | Original ID1: | | Cyp4e3 | | P Value: | | 1.53E-02 | | Note: | | Slow up-regulation, have mouse orthologs |
| | Dataset Information | | Name: | | 17054780 | | Method: | | Microarray | | Phenotype: | | Ethanol response | | Publication: | | Morozova et al. Genome Biol. (2006) Transcriptional response to alcohol exposure in Drosophila melanogaster. PubMed | | Summary: | | We assessed whole-genome transcriptional responses following alcohol exposure and demonstrate immediate down-regulation of genes affecting olfaction, rapid upregulation of biotransformation enzymes and, concomitant with development of tolerance, altered transcription of transcriptional regulators, proteases and metabolic enzymes, including biotransformation enzymes and enzymes associated with fatty acid biosynthesis. Functional tests of P-element disrupted alleles corresponding to genes with altered transcription implicated 75% of these in the response to alcohol, two-thirds of which have human orthologues. We performed analysis of variance to identify probe sets with significant differences in expression between the three treatments, and a false positive discovery rate of q < 0.05 to account for multiple tests. |
| | Gene Information | | Original ID1: | | Cyp4e3 | | Fold Change: | | >2 | | P Value: | | 9.38E-10 | | Note: | | Resistant line>Sensitive line |
| | Dataset Information | | Name: | | 17973985 | | Method: | | Microarray | | Phenotype: | | Ethanol sensitivity | | Publication: | | Morozova et al. Genome Biol. (2007) Phenotypic and transcriptional response to selection for alcohol sensitivity in Drosophila melanogaster. PubMed | | Summary: | | We performed artificial selection for alcohol sensitivity for 35 generations and created duplicate selection lines that are either highly sensitive or resistant to ethanol exposure along with unselected control lines. We used whole genome expression analysis to identify 1,678 probe sets with different expression levels between the divergent lines, pooled across replicates, at a false discovery rate of q < 0.001. We assessed to what extent genes with altered transcriptional regulation might be causally associated with ethanol sensitivity by measuring alcohol sensitivity of 37 co-isogenic P-element insertional mutations in 35 candidate genes, and found that 32 of these mutants differed in sensitivity to ethanol exposure from their co-isogenic controls. Furthermore, 23 of these novel genes have human orthologues. |
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| Gene Refseq Sequence Annotation |
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| Gene Ontology (GO) Annotation |
| GO ID | GO Term | Category | Evidence (PubMed) | |
| GO:0005792 | microsome | Cellular Component | NAS | |
| GO:0005811 | lipid particle | Cellular Component | IDA | |
| GO:0016020 | membrane | Cellular Component | NAS | |
| GO:0004497 | monooxygenase activity | Molecular Function | IEA | |
| GO:0005506 | iron ion binding | Molecular Function | IEA | |
| GO:0009055 | electron carrier activity | Molecular Function | ISS | |
| GO:0009055 | electron carrier activity | Molecular Function | NAS | |
| GO:0020037 | heme binding | Molecular Function | IEA | |
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| Other Database Cross Links |
| NCBI Entrez Gene: | | 34291 |
| FLYBASE: | | FBgn0015035 |
