| General information | Literature | Expression | Regulation | Mutation | Interaction |
|
Basic Information |
|
|---|---|
Gene ID | 79831 |
Name | KDM8 |
Synonymous | JMJD5;lysine (K)-specific demethylase 8;KDM8;lysine (K)-specific demethylase 8 |
Definition | jmjC domain-containing protein 5|jumonji domain containing 5|jumonji domain-containing protein 5|lysine-specific demethylase 8 |
Position | 16p12.1 |
Gene type | protein-coding |
|
Title |
Abstract |
| Tumor suppressor gene identification using retroviral insertional mutagenesis in Blm-deficient mice. | Retroviral insertional mutagenesis preferentially identifies oncogenes rather than tumor suppressor (TS) genes, presumably because a single retroviral-induced mutation is sufficient to activate an oncogene and initiate a tumor, whereas two mutations are needed to inactivate a TS gene. Here we show that TS genes can be identified by insertional mutagenesis when the screens are performed in Blm-deficient backgrounds. Blm-deficient mice, like Bloom syndrome patients, have increased frequencies of mitotic recombination owing to a mutation in the RecQ protein-like-3 helicase gene. This increased mitotic recombination increases the likelihood that an insertional mutation in one allele of a TS gene will become homozygoused by non-sister chromatid exchange and the homozygosity of the insertion provides a marker for identifying the TS gene. We also show that known as well as novel TS genes can be identified by insertional mutagenesis in Blm-deficient mice and identify two JmjC family proteins that contribute to genome stability in species as evolutionarily diverse as mammals and Caenorhabditis elegans. |