| General information | Literature | Expression | Regulation | Mutation | Interaction |
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Basic Information |
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Gene ID | 91608 |
Name | RASL10B |
Synonymous | RRP17|VTS58635;RAS-like, family 10, member B;RASL10B;RAS-like, family 10, member B |
Definition | Ras-related protein 17|ras-like protein VTS58635|ras-like protein family member 10B |
Position | 17q12 |
Gene type | protein-coding |
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Title |
Abstract |
| Cloning and characterization of a novel small monomeric GTPase, RasL10B, with tumor suppressor potential. | Ras proteins are members of the superfamily of small GTPase. A novel human Ras-like transcript, termed RasL10B, was isolated from human blood cell cDNA library. RasL10B gene contains four exons and three introns, which encodes a 203 amino acid protein with a molecular mass of about 23.2 kDa. RT-PCR analysis showed that RasL10B is expressed extensively in human tissues. Subcellular location analysis of GFP-RasL10B fusion protein revealed that RasL10B was distributed to the cytoplasm of COS7 cells. In addition, RasL10B was expressed in E. coli Rosette (DE3) and purified to a homogenicity by Ni-NTA affinity chromatography. Finally, the mRNA levels of RasL10B were down-regulated in all human breast cancer cell lines we tested. In summary, RasL10B is a new member of Ras superfamily with tumor suppressor potential. |