DegronMD

Uniprot ID: O14757

Source protein: Serine/threonine-protein kinase Chk1

Source gene: CHEK1

Class: DEG_SCF_FBW7_1

Location: 359:366

E3 ligase: FBXW7

DEGRON score: 0.9932

ASA_SCORE: 71.2

COIL: 0.8489

CONS_SCORE: 0.3046

DSS_SCORE: 0.4387

HELIX: 0.0949

FCONS_SCORE: 0.7895

RIG_SCORE: 0.6499

Degron related mutations and impact score

Mutation	Sample	Cancer	Dataset	Degron	Degron class	Impact score
p.G364V	DO231948	LICA	ICGC	LLGTPGSS	DEG_SCF_FBW7_1	0.6421
p.R376I	DO229015	LICA	ICGC	LLGTPGSS	DEG_SCF_FBW7_1	0.0556
p.Q374R	SIDM01551	Gastric Carcinoma	GDSC	LLGTPGSS	DEG_SCF_FBW7_1	0.1111
p.Q358R	ACH-000485	Gastric Cancer	CCLE	LLGTPGSS	DEG_SCF_FBW7_1	0.3056
p.G361D	ACH-000989	Colon/Colorectal Cancer	CCLE	LLGTPGSS	DEG_SCF_FBW7_1	0.6087
p.G361D	SNU-175	large_intestine	COSMIC	LLGTPGSS	DEG_SCF_FBW7_1	0.6087
p.D351A	ACH-001991	Primary	CCLE	LLGTPGSS	DEG_SCF_FBW7_1	0.1111
p.R375S	GP5d	large_intestine	COSMIC	LLGTPGSS	DEG_SCF_FBW7_1	0.1944

Drug responses associated with CHEK1 mutations

Sample	Cancer	Mutation (In Degron?)	Dataset	Drug	IC50
SIDM01135	PAAD\|pancreas	p.D139Y (No)	GDSC	AZD7762	2.9424
SIDM01135	PAAD\|pancreas	p.D139Y (No)	GDSC	Wee1 Inhibitor	14.4929
SIDM01135	PAAD\|pancreas	p.D139Y (No)	GDSC	AZD7762	1.6273
SIDM01135	PAAD\|pancreas	p.D139Y (No)	GDSC	AZD7762	3.4391
SIDM01135	PAAD\|pancreas	p.D139Y (No)	GDSC	Wee1 Inhibitor	78.11
SIDM01135	PAAD\|pancreas	p.D139Y (No)	GDSC	MK-8776	115.69
SIDM00388	UNCLASSIFIED\|B_cell_leukemia	p.T226fs*14 (No)	GDSC	AZD7762	0.0676
SIDM00388	UNCLASSIFIED\|B_cell_leukemia	p.T226fs*14 (No)	GDSC	Wee1 Inhibitor	1.6772
SIDM00388	UNCLASSIFIED\|B_cell_leukemia	p.T226fs*14 (No)	GDSC	AZD7762	0.0684
SIDM00388	UNCLASSIFIED\|B_cell_leukemia	p.T226fs*14 (No)	GDSC	AZD7762	0.2478
SIDM00388	UNCLASSIFIED\|B_cell_leukemia	p.T226fs*14 (No)	GDSC	Wee1 Inhibitor	2.207
SIDM00388	UNCLASSIFIED\|B_cell_leukemia	p.T226fs*14 (No)	GDSC	MK-8776	5.1065
SIDM00524	SCLC\|lung_small_cell_carcinoma	p.C87S (No)	GDSC	AZD7762	4.286
SIDM00524	SCLC\|lung_small_cell_carcinoma	p.C87S (No)	GDSC	Wee1 Inhibitor	20.0426
SIDM00524	SCLC\|lung_small_cell_carcinoma	p.C87S (No)	GDSC	AZD7762	3.3201
SIDM00250	ESCA\|oesophagus	p.D434Y (No)	GDSC	AZD7762	0.1425
SIDM00250	ESCA\|oesophagus	p.D434Y (No)	GDSC	AZD7762	0.2125
SIDM00250	ESCA\|oesophagus	p.D434Y (No)	GDSC	AZD7762	0.8342
SIDM00250	ESCA\|oesophagus	p.D434Y (No)	GDSC	Wee1 Inhibitor	8.5792
SIDM00250	ESCA\|oesophagus	p.D434Y (No)	GDSC	MK-8776	14.481
SIDM01106	SKCM\|melanoma	p.R453* (No)	GDSC	AZD7762	2.5056
SIDM01106	SKCM\|melanoma	p.R453* (No)	GDSC	Wee1 Inhibitor	17.1165
SIDM01106	SKCM\|melanoma	p.R453* (No)	GDSC	AZD7762	1.2103
SIDM01106	SKCM\|melanoma	p.R453* (No)	GDSC	AZD7762	6.838
SIDM01106	SKCM\|melanoma	p.R453* (No)	GDSC	Wee1 Inhibitor	54.451
SIDM01106	SKCM\|melanoma	p.R453* (No)	GDSC	MK-8776	127.59
SIDM00151	OV\|ovary	p.T226fs*14 (No)	GDSC	AZD7762	0.225
SIDM00151	OV\|ovary	p.T226fs*14 (No)	GDSC	Wee1 Inhibitor	4.9093
SIDM00151	OV\|ovary	p.T226fs*14 (No)	GDSC	AZD7762	44.8521
SIDM00151	OV\|ovary	p.T226fs*14 (No)	GDSC	AZD7762	0.252
SIDM00151	OV\|ovary	p.T226fs*14 (No)	GDSC	Wee1 Inhibitor	4.2025
SIDM00151	OV\|ovary	p.T226fs*14 (No)	GDSC	MK-8776	16.005
SIDM00946	ALL\|T_cell_leukemia	p.T226fs*14 (No)	GDSC	AZD7762	0.1682
SIDM00946	ALL\|T_cell_leukemia	p.T226fs*14 (No)	GDSC	Wee1 Inhibitor	5.5259
SIDM00946	ALL\|T_cell_leukemia	p.T226fs*14 (No)	GDSC	AZD7762	0.0539
SIDM00946	ALL\|T_cell_leukemia	p.T226fs*14 (No)	GDSC	AZD7762	0.1395
SIDM00946	ALL\|T_cell_leukemia	p.T226fs*14 (No)	GDSC	Wee1 Inhibitor	1.114
SIDM00946	ALL\|T_cell_leukemia	p.T226fs*14 (No)	GDSC	MK-8776	1.126
SIDM00933	BRCA\|breast	p.L355fs*1 (Yes)	GDSC	AZD7762	0.5811
SIDM00933	BRCA\|breast	p.L355fs*1 (Yes)	GDSC	Wee1 Inhibitor	4.8165
SIDM00933	BRCA\|breast	p.L355fs*1 (Yes)	GDSC	AZD7762	0.2933
SIDM00933	BRCA\|breast	p.L355fs*1 (Yes)	GDSC	AZD7762	0.4707
SIDM00933	BRCA\|breast	p.L355fs*1 (Yes)	GDSC	Wee1 Inhibitor	2.0199
SIDM00933	BRCA\|breast	p.L355fs*1 (Yes)	GDSC	MK-8776	19.543
SIDM00217	COREAD\|large_intestine	p.A116T (No)	GDSC	AZD7762	19.6882
SIDM00217	COREAD\|large_intestine	p.A116T (No)	GDSC	AZD7762	0.6235
SIDM00216	COREAD\|large_intestine	p.G361D (Yes)	GDSC	AZD7762	1.476
SIDM00216	COREAD\|large_intestine	p.G361D (Yes)	GDSC	Wee1 Inhibitor	13.041
SIDM00216	COREAD\|large_intestine	p.G361D (Yes)	GDSC	MK-8776	15.374
SIDM00660	COREAD\|large_intestine	p.A237D (No)	GDSC	AZD7762	0.4635
SIDM00660	COREAD\|large_intestine	p.A237D (No)	GDSC	Wee1 Inhibitor	15.9191
SIDM00660	COREAD\|large_intestine	p.A237D (No)	GDSC	AZD7762	0.463
SIDM00660	COREAD\|large_intestine	p.A237D (No)	GDSC	AZD7762	0.3309
SIDM00660	COREAD\|large_intestine	p.A237D (No)	GDSC	Wee1 Inhibitor	1.4298
SIDM00660	COREAD\|large_intestine	p.A237D (No)	GDSC	MK-8776	3.9501
SIDM00522	SCLC\|lung_small_cell_carcinoma	p.477 (No)	GDSC	AZD7762	1.0633
SIDM00522	SCLC\|lung_small_cell_carcinoma	p.477 (No)	GDSC	Wee1 Inhibitor	14.4381
SIDM00522	SCLC\|lung_small_cell_carcinoma	p.477 (No)	GDSC	AZD7762	0.5161
SIDM00522	SCLC\|lung_small_cell_carcinoma	p.477 (No)	GDSC	AZD7762	1.1226
SIDM00522	SCLC\|lung_small_cell_carcinoma	p.477 (No)	GDSC	Wee1 Inhibitor	6.7586
SIDM00522	SCLC\|lung_small_cell_carcinoma	p.477 (No)	GDSC	MK-8776	16.33
SIDM00683	PRAD\|prostate	p.T226fs*14 (No)	GDSC	AZD7762	1.4032
SIDM00683	PRAD\|prostate	p.T226fs*14 (No)	GDSC	Wee1 Inhibitor	9.3649
SIDM00683	PRAD\|prostate	p.T226fs*14 (No)	GDSC	AZD7762	0.618
SIDM00683	PRAD\|prostate	p.T226fs*14 (No)	GDSC	AZD7762	1.8957
SIDM00683	PRAD\|prostate	p.T226fs*14 (No)	GDSC	Wee1 Inhibitor	9.3378
SIDM00683	PRAD\|prostate	p.T226fs*14 (No)	GDSC	MK-8776	20.874
SIDM00114	MESO\|mesothelioma	p.E217Q (No)	GDSC	AZD7762	2.7003
SIDM00114	MESO\|mesothelioma	p.E217Q (No)	GDSC	Wee1 Inhibitor	17.3929
SIDM00114	MESO\|mesothelioma	p.E217Q (No)	GDSC	AZD7762	0.6874
SIDM00114	MESO\|mesothelioma	p.E217Q (No)	GDSC	AZD7762	4.1793
SIDM00114	MESO\|mesothelioma	p.E217Q (No)	GDSC	Wee1 Inhibitor	49.575
SIDM00114	MESO\|mesothelioma	p.E217Q (No)	GDSC	MK-8776	86.753
SIDM00537	COREAD\|large_intestine	p.R419S (No)	GDSC	AZD7762	0.5121
SIDM00537	COREAD\|large_intestine	p.R419S (No)	GDSC	Wee1 Inhibitor	3.1428
SIDM00537	COREAD\|large_intestine	p.R419S (No)	GDSC	AZD7762	0.5244
SIDM00537	COREAD\|large_intestine	p.R419S (No)	GDSC	AZD7762	0.3342
SIDM00537	COREAD\|large_intestine	p.R419S (No)	GDSC	Wee1 Inhibitor	3.8086
SIDM00537	COREAD\|large_intestine	p.R419S (No)	GDSC	MK-8776	13.263
SIDM00447	UNCLASSIFIED\|B_cell_leukemia	p.D209N (No)	GDSC	AZD7762	0.563
SIDM00447	UNCLASSIFIED\|B_cell_leukemia	p.D209N (No)	GDSC	Wee1 Inhibitor	1.8529
SIDM00447	UNCLASSIFIED\|B_cell_leukemia	p.D209N (No)	GDSC	AZD7762	0.3941
SIDM00447	UNCLASSIFIED\|B_cell_leukemia	p.D209N (No)	GDSC	AZD7762	0.7378
SIDM00447	UNCLASSIFIED\|B_cell_leukemia	p.D209N (No)	GDSC	Wee1 Inhibitor	4.8589
SIDM00447	UNCLASSIFIED\|B_cell_leukemia	p.D209N (No)	GDSC	MK-8776	14.294

DegronMD: protein-targeted degradation, mutation and drug response to degrons

Degron: LLGTPGSS

Degron related mutations and impact score

Drug responses associated with CHEK1 mutations

All proteins holding degron of LLGTPGSS

Uniprot ID	Source protein	Degron class	Location	Hit	E3 ligase	Degron score	Detail
O14757	Serine/threonine-protein kinase Chk1	DEG_SCF_FBW7_1	359:366	LLGTPGSS	FBXW7	0.9932	More...