FUNCTION: Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 (EEF2) and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta). {ECO:0000250}.; FUNCTION: Down-regulation increa...
FUNCTION: Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 (EEF2) and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A (Eta). {ECO:0000250}.; FUNCTION: Down-regulation increases extracellular release of proteoglycans, indicating a possible role in the secretion process. Stimulates binding of GNEFR to SEC5. {ECO:0000269|PubMed:14527407, ECO:0000269|PubMed:14980502}.
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GO - Biological processes (BP):
negative regulation of protein secretion [GO:0050709]; peptidyl-diphthamide biosynthetic process from peptidyl-histidine [GO:0017183]; positive regulation of binding [GO:0051099]; tRNA wobble uridine modification [GO:0002098]...
negative regulation of protein secretion [GO:0050709]; peptidyl-diphthamide biosynthetic process from peptidyl-histidine [GO:0017183]; positive regulation of binding [GO:0051099]; tRNA wobble uridine modification [GO:0002098]
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GO - Molecular function (MF):
metal ion binding [GO:0046872]...
metal ion binding [GO:0046872]
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GO - Cellular component (CC):
cytoplasm [GO:0005737]; cytosol [GO:0005829]; nucleoplasm [GO:0005654]; nucleus [GO:0005634]...
cytoplasm [GO:0005737]; cytosol [GO:0005829]; nucleoplasm [GO:0005654]; nucleus [GO:0005634]
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