mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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	Gene Summary
	Ligand Binding Site Mutation Information
	Protein Structure Related Information
	Gene Expression and Gene-Gene Network
	Phenotype Information
	Pharmacological Information
	Conservation Information for LBS

Gene summary for RRAS2

Gene summary

Basic gene Info.	Gene symbol	RRAS2
	Gene name	related RAS viral (r-ras) oncogene homolog 2
	Synonyms	TC21
	Cytomap	UCSC genome browser: 11p15.2
	Type of gene	protein-coding
	RefGenes	NM_001102669.2, NM_001177314.1,NM_001177315.1,NM_012250.5,
	Description	ras-like protein TC21ras-related protein R-Ras2teratocarcinoma oncogene
	Modification date	20141207
dbXrefs	MIM : 600098
	HGNC : HGNC
	Ensembl : ENSG00000133818
	HPRD : 02518
	Vega : OTTHUMG00000165756
Protein	UniProt: P62070 go to UniProt's Cross Reference DB Table
Expression	CleanEX: HS_RRAS2
	BioGPS: 22800
Pathway	NCI Pathway Interaction Database: RRAS2
	KEGG: RRAS2
	REACTOME: RRAS2
	Pathway Commons: RRAS2
Context	iHOP: RRAS2
	ligand binding site mutation search in PubMed: RRAS2
	UCL Cancer Institute: RRAS2
Assigned class in mutLBSgeneDB	C: This gene just belongs to mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

GO ID	GO Term	PubMed ID
GO:0001649	osteoblast differentiation	16210410

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Ligand binding site mutations for RRAS2

Lollipop-style diagram of mutations at LBS in amino-acid sequence.
We represented ligand binding site mutations only. (You can see big image via clicking.)

: non-synonymous mutation on LBS, Circle size denotes number of samples.

Cancer type specific mutLBS sorted by frequency

LBS	AAchange of nsSNV	Cancer type	# samples
A158	A158T	COAD	1

cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

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Protein structure related information for RRAS2

Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
(ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)

: nsSNV at non-LBS

: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.

LBS	AAchange of nsSNV	Relative stability change
A158	A158T	-0.671086

(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for RRAS2 from PDB

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Differential gene expression and gene-gene network for RRAS2

Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)

* Left PPI network was created from samples with mutations in the LBS of RRAS2 and the right PPI network was created from samples without mutations in the LBS of RRAS2. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.

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Phenotype information for RRAS2

Gene level disease information (DisGeNet)

Disease ID	Disease name	# PubMed	Association type
umls:C0023904	Liver Neoplasms, Experimental	1	Biomarker

Mutation level pathogenic information (ClinVar annotation)

Allele ID

AA change

Clinical significance

Origin

Phenotype IDs

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Pharmacological information for RRAS2

Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Drug information targeting mutLBSgene (Approved drugs only)

Drug status

DrugBank ID

Name

Type

Drug structure

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of RRAS2 go to BioLip

Ligand ID	Ligand short name	Ligand long name	PDB ID	PDB name	mutLBS
GDP		GUANOSINE-5'-DIPHOSPHATE	2ery	A	A158
GDP		GUANOSINE-5'-DIPHOSPHATE	2ery	B	A158

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Conservation information for LBS of RRAS2

Multiple alignments for P62070 in multiple species

LBS	AA sequence	# species	Species
A158	YMEASAKIRMN	2	Homo sapiens, Mus musculus
A158	FLETSAKQAIN	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
A158	YIECSAKLRVN	1	Drosophila melanogaster
A158	YVETSAKLRLN	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
A158	YIETSAKTQHN	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
A29	GVGKSALTIQF	3	Homo sapiens, Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Mus musculus
A29	GVGKSALTIQL	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Schizosaccharomyces pombe (strain 972 / ATCC 24843)
A29	GVGKSAITIQF	1	Drosophila melanogaster
A29	GVGKSCLTIQL	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
D130	IGNKADLDHQR	2	Homo sapiens, Mus musculus
D130	VGNKSDLENEK	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
D130	VGNKLDLENER	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
D130	VGNKCDLKHQQ	1	Drosophila melanogaster
D130	VANKCDLEAER	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
D130	VGNKYDLRGER	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
F39	FIQSYFVTDYD	3	Homo sapiens, Mus musculus, Drosophila melanogaster
F39	LTQSHFVDEYD	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
F39	FIQSYFVDEYD	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
F39	LIQSHFVDEYD	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
F39	LIQGHFLDEYD	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
G24	VVGGGGVGKSA	5	Homo sapiens, Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Mus musculus, Drosophila melanogaster
G24	VVGDGGVGKSA	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
G24	VVGGGGVGKSC	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
G26	GGGGVGKSALT	4	Homo sapiens, Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Mus musculus
G26	GGGGVGKSAIT	1	Drosophila melanogaster
G26	GDGGVGKSALT	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
G26	GGGGVGKSCLT	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
K128	ILIGNKADLDH	2	Homo sapiens, Mus musculus
K128	VVVGNKSDLEN	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
K128	VVVGNKLDLEN	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
K128	LMVGNKCDLKH	1	Drosophila melanogaster
K128	VLVANKCDLEA	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
K128	IIVGNKYDLRG	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
K159	MEASAKIRMNV	2	Homo sapiens, Mus musculus
K159	LETSAKQAINV	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
K159	IECSAKLRVNV	1	Drosophila melanogaster
K159	VETSAKLRLNV	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
K159	IETSAKTQHNV	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
K27	GGGVGKSALTI	4	Homo sapiens, Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Mus musculus
K27	GGGVGKSAITI	1	Drosophila melanogaster
K27	DGGVGKSALTI	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
K27	GGGVGKSCLTI	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
L131	GNKADLDHQRQ	2	Homo sapiens, Mus musculus
L131	GNKSDLENEKQ	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L131	GNKLDLENERQ	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L131	GNKCDLKHQQQ	1	Drosophila melanogaster
L131	ANKCDLEAERV	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L131	GNKYDLRGERV	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
N127	MILIGNKADLD	2	Homo sapiens, Mus musculus
N127	IVVVGNKSDLE	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
N127	VVVVGNKLDLE	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
N127	MLMVGNKCDLK	1	Drosophila melanogaster
N127	VVLVANKCDLE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
N127	MIIVGNKYDLR	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
S157	TYMEASAKIRM	2	Homo sapiens, Mus musculus
S157	PFLETSAKQAI	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
S157	PYIECSAKLRV	1	Drosophila melanogaster
S157	LYVETSAKLRL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
S157	KYIETSAKTQH	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
S28	GGVGKSALTIQ	5	Homo sapiens, Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Mus musculus, Schizosaccharomyces pombe (strain 972 / ATCC 24843)
S28	GGVGKSAITIQ	1	Drosophila melanogaster
S28	GGVGKSCLTIQ	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
T41	QSYFVTDYDPT	3	Homo sapiens, Mus musculus, Drosophila melanogaster
T41	QSHFVDEYDPT	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Schizosaccharomyces pombe (strain 972 / ATCC 24843)
T41	QSYFVDEYDPT	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
T41	QGHFLDEYDPT	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
V25	VGGGGVGKSAL	4	Homo sapiens, Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Mus musculus
V25	VGGGGVGKSAI	1	Drosophila melanogaster
V25	VGDGGVGKSAL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
V25	VGGGGVGKSCL	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
V40	IQSYFVTDYDP	3	Homo sapiens, Mus musculus, Drosophila melanogaster
V40	TQSHFVDEYDP	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
V40	IQSYFVDEYDP	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
V40	IQSHFVDEYDP	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
V40	IQGHFLDEYDP	1	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)