mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

Home

	Gene Summary
	Ligand Binding Site Mutation Information
	Protein Structure Related Information
	Gene Expression and Gene-Gene Network
	Phenotype Information
	Pharmacological Information
	Conservation Information for LBS

Gene summary for ALOX15B

Gene summary

Basic gene Info.	Gene symbol	ALOX15B
	Gene name	arachidonate 15-lipoxygenase, type B
	Synonyms	15-LOX-2
	Cytomap	UCSC genome browser: 17p13.1
	Type of gene	protein-coding
	RefGenes	NM_001039130.1, NM_001039131.1,NM_001141.2,
	Description	15-LOX-B15S-lipoxygenasearachidonate 15-lipoxygenase 2arachidonate 15-lipoxygenase Barachidonate 15-lipoxygenase type IIarachidonate 15-lipoxygenase, second typearachidonate omega(6) lipoxygenaselinoleate 13-lipoxygenase 15-LOb
	Modification date	20141207
dbXrefs	MIM : 603697
	HGNC : HGNC
	Ensembl : ENSG00000179593
	HPRD : 04739
	Vega : OTTHUMG00000108181
Protein	UniProt: O15296 go to UniProt's Cross Reference DB Table
Expression	CleanEX: HS_ALOX15B
	BioGPS: 247
Pathway	NCI Pathway Interaction Database: ALOX15B
	KEGG: ALOX15B
	REACTOME: ALOX15B
	Pathway Commons: ALOX15B
Context	iHOP: ALOX15B
	ligand binding site mutation search in PubMed: ALOX15B
	UCL Cancer Institute: ALOX15B
Assigned class in mutLBSgeneDB	B: This gene belongs to targetable_mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

GO ID	GO Term	PubMed ID
GO:0008285	negative regulation of cell proliferation	11839751
GO:0019369	arachidonic acid metabolic process	10542053
GO:0045786	negative regulation of cell cycle	11839751
GO:0045926	negative regulation of growth	11839751

Top

Ligand binding site mutations for ALOX15B

Cancer type specific mutLBS sorted by frequency

LBS	AAchange of nsSNV	Cancer type	# samples
G17	G17D	BRCA	1
N40,G42	L41F	LUAD	1

cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

Top

Protein structure related information for ALOX15B

Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
(ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)

: nsSNV at non-LBS

: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.

LBS	AAchange of nsSNV	Relative stability change
N40	L41F	-1.3210453
G42	L41F	-1.3210453
G17	G17D	-0.46832631

(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for ALOX15B from PDB

Top

Differential gene expression and gene-gene network for ALOX15B

Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)

* Left PPI network was created from samples with mutations in the LBS of ALOX15B and the right PPI network was created from samples without mutations in the LBS of ALOX15B. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.

Top

Phenotype information for ALOX15B

Gene level disease information (DisGeNet)

Disease ID

Disease name

# PubMed

Association type

Mutation level pathogenic information (ClinVar annotation)

Allele ID

AA change

Clinical significance

Origin

Phenotype IDs

Top

Pharmacological information for ALOX15B

Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Drug information targeting mutLBSgene (Approved drugs only)

Drug status

DrugBank ID

Name

Type

Drug structure

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of ALOX15B go to BioLip

Ligand ID	Ligand short name	Ligand long name	PDB ID	PDB name	mutLBS
CA		CALCIUM(2+)	4nre	A	G17
CA		CALCIUM(2+)	4nre	A	N40 G42

Top

Conservation information for LBS of ALOX15B

Multiple alignments for O15296 in multiple species

LBS	AA sequence	# species	Species
A86	PLAPDAWFCRW	1	Homo sapiens
A86	SFRSDAWFCRW	1	Mus musculus
A86	SFPPDAWYCRW	1	Rattus norvegicus
D39	PLVPLDHLGKE	2	Mus musculus, Rattus norvegicus
D39	PPLPLDNLGKE	1	Homo sapiens
D85	GPLAPDAWFCR	1	Homo sapiens
D85	MSFRSDAWFCR	1	Mus musculus
D85	LSFPPDAWYCR	1	Rattus norvegicus
E44	DHLGKEFSAGA	2	Mus musculus, Rattus norvegicus
E44	DNLGKEFTAGA	1	Homo sapiens
G15	TGEACGAGTWD	2	Mus musculus, Rattus norvegicus
G15	TGEAFGAGTWD	1	Homo sapiens
G17	EACGAGTWDKV	2	Mus musculus, Rattus norvegicus
G17	EAFGAGTWDKV	1	Homo sapiens
G42	PLDHLGKEFSA	2	Mus musculus, Rattus norvegicus
G42	PLDNLGKEFTA	1	Homo sapiens
H373	HEALTHLLHSH	1	Homo sapiens
H373	HEAVTHLLHAH	1	Mus musculus
H373	HEALTHLLHAH	1	Rattus norvegicus
H378	HLLHAHLIPEV	2	Mus musculus, Rattus norvegicus
H378	HLLHSHLLPEV	1	Homo sapiens
H553	TCSAKHAAVSA	2	Homo sapiens, Rattus norvegicus
H553	TCSAKHAAVSS	1	Mus musculus
I26	KVSVSIVGTHG	2	Mus musculus, Rattus norvegicus
I26	KVSVSIVGTRG	1	Homo sapiens
N40	LVPLDHLGKEF	2	Mus musculus, Rattus norvegicus
N40	PLPLDNLGKEF	1	Homo sapiens