mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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Gene Summary

Ligand Binding Site Mutation Information

Protein Structure Related Information

Gene Expression and Gene-Gene Network

Phenotype Information

Pharmacological Information

Conservation Information for LBS

Gene summary for GRK4
Gene summary
Basic gene Info.Gene symbolGRK4
Gene nameG protein-coupled receptor kinase 4
SynonymsGPRK2L|GPRK4|GRK4a|IT11
CytomapUCSC genome browser: 4p16.3
Type of geneprotein-coding
RefGenesNM_001004056.1,
NM_001004057.1,NM_005307.2,NM_182982.2,
DescriptionITI1
Modification date20141207
dbXrefs MIM : 137026
HGNC : HGNC
Ensembl : ENSG00000125388
HPRD : 00656
Vega : OTTHUMG00000159914
ProteinUniProt: P32298
go to UniProt's Cross Reference DB Table
ExpressionCleanEX: HS_GRK4
BioGPS: 2868
PathwayNCI Pathway Interaction Database: GRK4
KEGG: GRK4
REACTOME: GRK4
Pathway Commons: GRK4
ContextiHOP: GRK4
ligand binding site mutation search in PubMed: GRK4
UCL Cancer Institute: GRK4
Assigned class in mutLBSgeneDBB: This gene belongs to targetable_mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez
GO IDGO TermPubMed ID
GO:0031623receptor internalization20074556


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Ligand binding site mutations for GRK4
Lollipop-style diagram of mutations at LBS in amino-acid sequence.
We represented ligand binding site mutations only. (You can see big image via clicking.)
 
: non-synonymous mutation on LBS, Circle size denotes number of samples.

Cancer type specific mutLBS sorted by frequency
LBSAAchange of nsSNVCancer type# samples
G197G197ALUAD1
cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.


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Protein structure related information for GRK4
Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
  (ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)
: nsSNV at non-LBS: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.
LBSAAchange of nsSNVRelative stability change
G197G197A-0.89186056
(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for GRK4 from PDB

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Differential gene expression and gene-gene network for GRK4
Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)
* Left PPI network was created from samples with mutations in the LBS of GRK4 and the right PPI network was created from samples without mutations in the LBS of GRK4. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.


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Phenotype information for GRK4
Gene level disease information (DisGeNet)
Disease IDDisease name# PubMedAssociation type

Mutation level pathogenic information (ClinVar annotation)
Allele IDAA changeClinical significanceOriginPhenotype IDs

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Pharmacological information for GRK4
Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Gene-centered drug-gene interaction network
Drug information targeting mutLBSgene (Approved drugs only)
Drug statusDrugBank IDNameTypeDrug structure

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of GRK4 go to BioLip
Ligand IDLigand short nameLigand long namePDB IDPDB namemutLBS
AN2AMP PHOSPHORAMIDATE4yhjAG197
AN2AMP PHOSPHORAMIDATE4yhjBG197


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Conservation information for LBS of GRK4
Multiple alignments for P32298 in multiple species
LBSAA sequence# speciesSpecies
A214TGKMYACKKLE2Mus musculus, Rattus norvegicus
A214TGKMYACKKLQ1Homo sapiens
D330HIRISDLGLAT1Homo sapiens
D330HIRISDLGLAM1Mus musculus
D330HIRISDLGLAL1Rattus norvegicus
G194QYRVLGKGGFG2Mus musculus, Rattus norvegicus
G194HYRVLGKGGFG1Homo sapiens
G196RVLGKGGFGEV3Homo sapiens, Mus musculus, Rattus norvegicus
G197VLGKGGFGEVC3Homo sapiens, Mus musculus, Rattus norvegicus
K195YRVLGKGGFGE3Homo sapiens, Mus musculus, Rattus norvegicus
K216KMYACKKLEKK2Mus musculus, Rattus norvegicus
K216KMYACKKLQKK1Homo sapiens
L319KPENILLDDHG2Mus musculus, Rattus norvegicus
L319KPENILLDDRG1Homo sapiens
M267LVLTIMNGGDL3Homo sapiens, Mus musculus, Rattus norvegicus
T265LCLVLTIMNGG3Homo sapiens, Mus musculus, Rattus norvegicus
V201GGFGEVCACQV3Homo sapiens, Mus musculus, Rattus norvegicus


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