mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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	Gene Summary
	Ligand Binding Site Mutation Information
	Protein Structure Related Information
	Gene Expression and Gene-Gene Network
	Phenotype Information
	Pharmacological Information
	Conservation Information for LBS

Gene summary for GSTA1

Gene summary

Basic gene Info.	Gene symbol	GSTA1
	Gene name	glutathione S-transferase alpha 1
	Synonyms	GST2\|GSTA1-1\|GTH1
	Cytomap	UCSC genome browser: 6p12.1
	Type of gene	protein-coding
	RefGenes	NM_145740.3,
	Description	GST HA subunit 1GST class-alpha member 1GST, class alpha, 1GST-epsilonS-(hydroxyalkyl)glutathione lyase A1glutathione S-alkyltransferase A1glutathione S-aryltransferase A1glutathione S-transferase 2glutathione S-transferase A1glutathione S-transf
	Modification date	20141222
dbXrefs	MIM : 138359
	HGNC : HGNC
	HPRD : 00708
Protein	UniProt: P09210 go to UniProt's Cross Reference DB Table
Expression	CleanEX: HS_GSTA1
	BioGPS: 2938
Pathway	NCI Pathway Interaction Database: GSTA1
	KEGG: GSTA1
	REACTOME: GSTA1
	Pathway Commons: GSTA1
Context	iHOP: GSTA1
	ligand binding site mutation search in PubMed: GSTA1
	UCL Cancer Institute: GSTA1
Assigned class in mutLBSgeneDB	C: This gene just belongs to mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

GO ID	GO Term	PubMed ID
GO:0006749	glutathione metabolic process	11851347

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Ligand binding site mutations for GSTA1

Lollipop-style diagram of mutations at LBS in amino-acid sequence.
We represented ligand binding site mutations only. (You can see big image via clicking.)

: non-synonymous mutation on LBS, Circle size denotes number of samples.

Cancer type specific mutLBS sorted by frequency

LBS	AAchange of nsSNV	Cancer type	# samples
L107	L107V	BLCA	1
E210	E210D	COAD	1
V111	V111I	GBM	1
R131	R131H	GBM	1
E104,I106	M105I	HNSC	1
T68	T68A	STAD	1
R131	R131C	UCEC	1

cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

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Protein structure related information for GSTA1

Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
(ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)

: nsSNV at non-LBS

: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.

LBS	AAchange of nsSNV	Relative stability change
F30	E32D	-1.6778572
F220	R221S	-1.4098483
P56	V58A	-1.3042336
L107	L107F	-0.98076172
V55	V55E	-0.94567296

(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for GSTA1 from PDB

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Differential gene expression and gene-gene network for GSTA1

Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)

* Left PPI network was created from samples with mutations in the LBS of GSTA1 and the right PPI network was created from samples without mutations in the LBS of GSTA1. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.

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Phenotype information for GSTA1

Gene level disease information (DisGeNet)

Disease ID	Disease name	# PubMed	Association type
umls:C0033578	Prostatic Neoplasms	1	Biomarker, GeneticVariation
umls:C0400966	Non-alcoholic Fatty Liver Disease	1	Biomarker
umls:C0151744	Myocardial Ischemia	1	Biomarker

Mutation level pathogenic information (ClinVar annotation)

Allele ID

AA change

Clinical significance

Origin

Phenotype IDs

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Pharmacological information for GSTA1

Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Gene-centered drug-gene interaction network

Drug information targeting mutLBSgene (Approved drugs only)

Drug status	DrugBank ID	Name	Type	Drug structure
Approved\|nutraceutical	DB00143	Glutathione	Small molecule
Approved\|vet_approved	DB00608	Chloroquine	Small molecule

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of GSTA1 go to BioLip

Ligand ID	Ligand short name	Ligand long name	PDB ID	PDB name	mutLBS
ASD		ANDROST-4-ENE-3,17-DIONE	2vct	B	L107
ASD		ANDROST-4-ENE-3,17-DIONE	2vct	C	L107
ASD		ANDROST-4-ENE-3,17-DIONE	2vct	A	L107 F220
GSH		GLUTATHIONATE(1-)	2wju	B	V55 F220
GSH		GLUTATHIONATE(1-)	2wju	C	V55 F220
GSH		GLUTATHIONATE(1-)	2wju	D	V55 F220
GSH		GLUTATHIONATE(1-)	2wju	E	V55 F220
GSH		GLUTATHIONATE(1-)	2wju	H	V55 F220
GSH		GLUTATHIONATE(1-)	4acs	D	V55 F220
GSH		GLUTATHIONATE(1-)	2wju	A	V55 P56 F220
GSH		GLUTATHIONATE(1-)	2wju	F	V55 P56 F220
GSH		GLUTATHIONATE(1-)	2wju	G	V55 P56 F220
GSH		GLUTATHIONATE(1-)	4acs	A	V55 P56 F220
GSH		GLUTATHIONATE(1-)	4acs	B	V55 P56 F220
GSH		GLUTATHIONATE(1-)	4acs	C	V55 P56 F220

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Conservation information for LBS of GSTA1

Multiple alignments for P09210 in multiple species

LBS

AA sequence

# species

Species