mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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	Gene Summary
	Ligand Binding Site Mutation Information
	Protein Structure Related Information
	Gene Expression and Gene-Gene Network
	Phenotype Information
	Pharmacological Information
	Conservation Information for LBS

Gene summary for PPP3CB

Gene summary

Basic gene Info.	Gene symbol	PPP3CB
	Gene name	protein phosphatase 3, catalytic subunit, beta isozyme
	Synonyms	CALNA2\|CALNB\|CNA2\|PP2Bbeta
	Cytomap	UCSC genome browser: 10q22.2
	Type of gene	protein-coding
	RefGenes	NM_001142353.2, NM_001142354.2,NM_001289968.1,NM_001289969.1,NM_021132.3,
	Description	CAM-PRP catalytic subunitcalcineurin A betacalcineurin A2calmodulin-dependent calcineurin A subunit beta isoformprotein phosphatase 2B, catalytic subunit, beta isoformprotein phosphatase 3 (formerly 2B), catalytic subunit, beta isoformprotein phosph
	Modification date	20141207
dbXrefs	MIM : 114106
	HGNC : HGNC
	Ensembl : ENSG00000107758
	HPRD : 00235
	Vega : OTTHUMG00000018472
Protein	UniProt: P16298 go to UniProt's Cross Reference DB Table
Expression	CleanEX: HS_PPP3CB
	BioGPS: 5532
Pathway	NCI Pathway Interaction Database: PPP3CB
	KEGG: PPP3CB
	REACTOME: PPP3CB
	Pathway Commons: PPP3CB
Context	iHOP: PPP3CB
	ligand binding site mutation search in PubMed: PPP3CB
	UCL Cancer Institute: PPP3CB
Assigned class in mutLBSgeneDB	C: This gene just belongs to mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

GO ID	GO Term	PubMed ID
GO:0033173	calcineurin-NFAT signaling cascade	22688515
GO:0035690	cellular response to drug	11005320
GO:0045944	positive regulation of transcription from RNA polymerase II promoter	22688515
GO:0051533	positive regulation of NFAT protein import into nucleus	22688515

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Ligand binding site mutations for PPP3CB

Cancer type specific mutLBS sorted by frequency

LBS	AAchange of nsSNV	Cancer type	# samples
E490	E490Q	LUSC	1

cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

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Protein structure related information for PPP3CB

Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
(ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)

: nsSNV at non-LBS

: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.

LBS	AAchange of nsSNV	Relative stability change
E490	E490Q	-0.76189682

(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for PPP3CB from PDB

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Differential gene expression and gene-gene network for PPP3CB

Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)

* Left PPI network was created from samples with mutations in the LBS of PPP3CB and the right PPI network was created from samples without mutations in the LBS of PPP3CB. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.

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Phenotype information for PPP3CB

Gene level disease information (DisGeNet)

Disease ID

Disease name

# PubMed

Association type

Mutation level pathogenic information (ClinVar annotation)

Allele ID

AA change

Clinical significance

Origin

Phenotype IDs

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Pharmacological information for PPP3CB

Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Drug information targeting mutLBSgene (Approved drugs only)

Drug status

DrugBank ID

Name

Type

Drug structure

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of PPP3CB go to BioLip

Ligand ID	Ligand short name	Ligand long name	PDB ID	PDB name	mutLBS
FE		IRON(3+)	4ora	A	E490

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Conservation information for LBS of PPP3CB

Multiple alignments for P16298 in multiple species

LBS	AA sequence	# species	Species
D127	YLFLGDYVDRG	5	Homo sapiens, Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Drosophila melanogaster, Mus musculus, Rattus norvegicus
D99	ITVCGDIHGQF	4	Homo sapiens, Drosophila melanogaster, Mus musculus, Rattus norvegicus
D99	ITVCGDIHGQY	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E490	LDRINERMPPR	3	Homo sapiens, Mus musculus, Rattus norvegicus
E490	HDLINEKLPPS	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E490	LDAVNERMPPR	1	Drosophila melanogaster
H101	VCGDIHGQFFD	4	Homo sapiens, Drosophila melanogaster, Mus musculus, Rattus norvegicus
H101	VCGDIHGQYFD	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
H208	QFLCVHGGLSP	3	Homo sapiens, Mus musculus, Rattus norvegicus
H208	QYLCVHGGISP	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
H208	QFLCIHGGLSP	1	Drosophila melanogaster
H290	SIIRAHEAQDA	4	Homo sapiens, Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Mus musculus, Rattus norvegicus
H290	SIVRAHEAQDA	1	Drosophila melanogaster
N159	FLLRGNHECRH	3	Homo sapiens, Mus musculus, Rattus norvegicus
N159	WLLRGNHECKH	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
N159	SLLRGNHECRH	1	Drosophila melanogaster
Y320	FSAPNYLDVYN	4	Homo sapiens, Drosophila melanogaster, Mus musculus, Rattus norvegicus
Y320	FSAPNYLDTYN	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)