mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

Home

	Gene Summary
	Ligand Binding Site Mutation Information
	Protein Structure Related Information
	Gene Expression and Gene-Gene Network
	Phenotype Information
	Pharmacological Information
	Conservation Information for LBS

Gene summary for PRKAR2A

Gene summary

Basic gene Info.	Gene symbol	PRKAR2A
	Gene name	protein kinase, cAMP-dependent, regulatory, type II, alpha
	Synonyms	PKR2\|PRKAR2
	Cytomap	UCSC genome browser: 3p21.3-p21.2
	Type of gene	protein-coding
	RefGenes	NM_004157.2,
	Description	cAMP-dependent protein kinase regulatory subunit RII alphacAMP-dependent protein kinase type II-alpha regulatory subunitprotein kinase A, RII-alpha subunit
	Modification date	20141207
dbXrefs	MIM : 176910
	HGNC : HGNC
	Ensembl : ENSG00000114302
	HPRD : 01484
	Vega : OTTHUMG00000133540
Protein	UniProt: P13861 go to UniProt's Cross Reference DB Table
Expression	CleanEX: HS_PRKAR2A
	BioGPS: 5576
Pathway	NCI Pathway Interaction Database: PRKAR2A
	KEGG: PRKAR2A
	REACTOME: PRKAR2A
	Pathway Commons: PRKAR2A
Context	iHOP: PRKAR2A
	ligand binding site mutation search in PubMed: PRKAR2A
	UCL Cancer Institute: PRKAR2A
Assigned class in mutLBSgeneDB	C: This gene just belongs to mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

GO ID	GO Term	PubMed ID
GO:2000480	negative regulation of cAMP-dependent protein kinase activity	21812984

Top

Ligand binding site mutations for PRKAR2A

Cancer type specific mutLBS sorted by frequency

LBS	AAchange of nsSNV	Cancer type	# samples
R23	R23Q	LUAD	1

cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

Top

Protein structure related information for PRKAR2A

Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
(ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)

: nsSNV at non-LBS

: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.

LBS	AAchange of nsSNV	Relative stability change
R23	R23Q	-0.88756321

(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for PRKAR2A from PDB

Top

Differential gene expression and gene-gene network for PRKAR2A

Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)

* Left PPI network was created from samples with mutations in the LBS of PRKAR2A and the right PPI network was created from samples without mutations in the LBS of PRKAR2A. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.

Top

Phenotype information for PRKAR2A

Gene level disease information (DisGeNet)

Disease ID

Disease name

# PubMed

Association type

Mutation level pathogenic information (ClinVar annotation)

Allele ID

AA change

Clinical significance

Origin

Phenotype IDs

Top

Pharmacological information for PRKAR2A

Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Gene-centered drug-gene interaction network

Drug information targeting mutLBSgene (Approved drugs only)

Drug status	DrugBank ID	Name	Type	Drug structure
Investigational	DB05798	GEM-231	Small molecule

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of PRKAR2A go to BioLip

Ligand ID

Ligand short name

Ligand long name

PDB ID

PDB name

mutLBS

Top

Conservation information for LBS of PRKAR2A

Multiple alignments for P13861 in multiple species

LBS	AA sequence	# species	Species
A43	-RLREARAP--	1	Homo sapiens
A43	-KLQSER----	1	Drosophila melanogaster
A43	-RLREAR----	1	Rattus norvegicus
A43	-RLREARSR--	1	Bos taurus
A43	EKLKAERSVFR	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
A43	--LEEEEADGI	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
A43	KKLEEERLFVR	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
A43	KRLEQQRAFLK	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
A43	KRLEQQRTFVR	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
E35	VEFAVEYFT-R	1	Homo sapiens
E35	IDYAVEYFT-K	1	Drosophila melanogaster
E35	VDFAVEYFT-R	1	Rattus norvegicus
E35	VDFAVDYFT-R	1	Bos taurus
E35	LQFCYDFFGEK	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
E35	IQPV-------	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
E35	LQFAANYFNKK	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
E35	LQFSANYFNKR	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E35	LQFAANYFTKR	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
F32	PDLVEFAVEYF	1	Homo sapiens
F32	PDVIDYAVEYF	1	Drosophila melanogaster
F32	PDLVDFAVEYF	1	Rattus norvegicus
F32	PDLVDFAVDYF	1	Bos taurus
F32	SDVLQFCYDFF	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
F32	AGSIQPV----	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
F32	GDLLQFAANYF	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
F32	SDFLQFSANYF	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
F32	NDFLQFAANYF	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
I4	--MSHIQIPPG	3	Homo sapiens, Rattus norvegicus, Bos taurus
I4	DSSRRIQVPEE	1	Drosophila melanogaster
I4	---------MS	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
I4	--MSASGFTSP	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
I4	-----MVLSQE	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
I4	---MVSSLPKE	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
I4	--------MAT	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
I6	MSHIQIPPGLT	3	Homo sapiens, Rattus norvegicus, Bos taurus
I6	SRRIQVPEELK	1	Drosophila melanogaster
I6	-------MSFE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
I6	MSASGFTSPFG	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
I6	---MVLSQEHR	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
I6	-MVSSLPKESQ	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
I6	------MATYQ	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
L14	GLTELLQGYTV	3	Homo sapiens, Rattus norvegicus, Bos taurus
L14	ELKEVLLQFSI	1	Drosophila melanogaster
L14	SFEEVYEELKA	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L14	PFGANSNPFGS	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
L14	EHRDLLDAFQK	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
L14	ESQAELQLFQN	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L14	TYQTELDLFKN	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
L40	YFT-RLREARA	1	Homo sapiens
L40	YFT-KLQSER-	1	Drosophila melanogaster
L40	YFT-RLREAR-	1	Rattus norvegicus
L40	YFT-RLREARS	1	Bos taurus
L40	FFGEKLKAERS	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L40	-----LEEEEA	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
L40	YFNKKLEEERL	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
L40	YFNKRLEQQRA	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L40	YFTKRLEQQRT	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Q15	LTELLQGYTVE	3	Homo sapiens, Rattus norvegicus, Bos taurus
Q15	LKEVLLQFSIS	1	Drosophila melanogaster
Q15	FEEVYEELKAL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Q15	FGANSNPFGSP	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
Q15	HRDLLDAFQKE	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
Q15	SQAELQLFQNE	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Q15	YQTELDLFKNE	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Q24	VEVLRQQPPDL	2	Homo sapiens, Rattus norvegicus
Q24	ISFLVEQPPDV	1	Drosophila melanogaster
Q24	VEVLRQRPPDL	1	Bos taurus
Q24	ALVDEQNPSDV	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Q24	SPEERRGVAGS	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
Q24	KEVEQRRPGDL	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
Q24	NEINAANPSDF	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Q24	NEVEQKQPNDF	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
R23	TVEVLRQQPPD	2	Homo sapiens, Rattus norvegicus
R23	SISFLVEQPPD	1	Drosophila melanogaster
R23	TVEVLRQRPPD	1	Bos taurus
R23	KALVDEQNPSD	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
R23	GSPEERRGVAG	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
R23	QKEVEQRRPGD	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
R23	QNEINAANPSD	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
R23	KNEVEQKQPND	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
R39	EYFT-RLREAR	2	Homo sapiens, Rattus norvegicus
R39	EYFT-KLQSER	1	Drosophila melanogaster
R39	DYFT-RLREAR	1	Bos taurus
R39	DFFGEKLKAER	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
R39	------LEEEE	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
R39	NYFNKKLEEER	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
R39	NYFNKRLEQQR	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
R39	NYFTKRLEQQR	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
T11	IPPGLTELLQG	3	Homo sapiens, Rattus norvegicus, Bos taurus
T11	VPEELKEVLLQ	1	Drosophila melanogaster
T11	--MSFEEVYEE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
T11	FTSPFGANSNP	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
T11	LSQEHRDLLDA	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
T11	LPKESQAELQL	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
T11	-MATYQTELDL	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
T18	LLQGYTVEVLR	3	Homo sapiens, Rattus norvegicus, Bos taurus
T18	VLLQFSISFLV	1	Drosophila melanogaster
T18	VYEELKALVDE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
T18	NSNPFGSPEER	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
T18	LLDAFQKEVEQ	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
T18	ELQLFQNEINA	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
T18	ELDLFKNEVEQ	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
V19	LQGYTVEVLRQ	3	Homo sapiens, Rattus norvegicus, Bos taurus
V19	LLQFSISFLVE	1	Drosophila melanogaster
V19	YEELKALVDEQ	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
V19	SNPFGSPEERR	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
V19	LDAFQKEVEQR	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
V19	LQLFQNEINAA	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
V19	LDLFKNEVEQK	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Y36	EFAVEYFT-RL	1	Homo sapiens
Y36	DYAVEYFT-KL	1	Drosophila melanogaster
Y36	DFAVEYFT-RL	1	Rattus norvegicus
Y36	DFAVDYFT-RL	1	Bos taurus
Y36	QFCYDFFGEKL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Y36	QPV-------L	1	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
Y36	QFAANYFNKKL	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
Y36	QFSANYFNKRL	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Y36	QFAANYFTKRL	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)