mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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Gene Summary

Ligand Binding Site Mutation Information

Protein Structure Related Information

Gene Expression and Gene-Gene Network

Phenotype Information

Pharmacological Information

Conservation Information for LBS

Gene summary for RPA2
Gene summary
Basic gene Info.Gene symbolRPA2
Gene namereplication protein A2, 32kDa
SynonymsREPA2|RP-A p32|RP-A p34|RPA32
CytomapUCSC genome browser: 1p35
Type of geneprotein-coding
RefGenesNM_001286076.1,
NM_001297558.1,NM_002946.4,
DescriptionRF-A protein 2replication factor A protein 2replication protein A 32 kDa subunitreplication protein A 34 kDa subunit
Modification date20141207
dbXrefs MIM : 179836
HGNC : HGNC
Ensembl : ENSG00000117748
HPRD : 01566
Vega : OTTHUMG00000003915
ProteinUniProt: P15927
go to UniProt's Cross Reference DB Table
ExpressionCleanEX: HS_RPA2
BioGPS: 6118
PathwayNCI Pathway Interaction Database: RPA2
KEGG: RPA2
REACTOME: RPA2
Pathway Commons: RPA2
ContextiHOP: RPA2
ligand binding site mutation search in PubMed: RPA2
UCL Cancer Institute: RPA2
Assigned class in mutLBSgeneDBC: This gene just belongs to mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez
GO IDGO TermPubMed ID
GO:0006260DNA replication2406247
GO:0006284base-excision repair9765279


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Ligand binding site mutations for RPA2
Lollipop-style diagram of mutations at LBS in amino-acid sequence.
We represented ligand binding site mutations only. (You can see big image via clicking.)
 : non-synonymous mutation on LBS, Circle size denotes number of samples.

Cancer type specific mutLBS sorted by frequency
LBSAAchange of nsSNVCancer type# samples
L149P148SGBM1
D61L59FLUAD1
T110D109GSKCM1
D61L59FSKCM1
D261D261ASTAD1
I255I255VUCEC1
cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.


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Protein structure related information for RPA2
Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
  (ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)
: nsSNV at non-LBS: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.
LBSAAchange of nsSNVRelative stability change
L149P148S-1.3559296
D261D261A-0.97429503
T110D109G-0.93202477
I255I255V-0.89293111
D61L59F-0.54111407
(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for RPA2 from PDB

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Differential gene expression and gene-gene network for RPA2
Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)
* Left PPI network was created from samples with mutations in the LBS of RPA2 and the right PPI network was created from samples without mutations in the LBS of RPA2. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.


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Phenotype information for RPA2
Gene level disease information (DisGeNet)
Disease IDDisease name# PubMedAssociation type

Mutation level pathogenic information (ClinVar annotation)
Allele IDAA changeClinical significanceOriginPhenotype IDs

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Pharmacological information for RPA2
Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.
Drug information targeting mutLBSgene (Approved drugs only)
Drug statusDrugBank IDNameTypeDrug structure

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of RPA2 go to BioLip
Ligand IDLigand short nameLigand long namePDB IDPDB namemutLBS
IIIPeptide ligand (ARG,ILE,GLN,ARG,ASN,LYS,ALA,ALA,ALA,LEU,LEU,ARG,LEU,ALA,ALA,ARG)1dpuAD261
IIIPeptide ligand (LEU,THR,GLU,GLU,GLN,ARG,LYS,LYS,ILE,GLU,GLU,ASN,ARG,GLN,LYS,ALA,LEU,ALA,ARG,ARG,ALA,GLU,LYS,LEU,LEU,ALA)4mqvAI255
DIO1,4-DIOXANE2pi2AL149
DIO1,4-DIOXANE2pi2BL149


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Conservation information for LBS of RPA2
Multiple alignments for P15927 in multiple species
LBSAA sequence# speciesSpecies
D260IYSTVDDDHFK2Homo sapiens, Rattus norvegicus
D260IYSTIDEYHFK1Arabidopsis thaliana
D260IYSTLDETCFK1Arabidopsis thaliana
D260IYSTIDESHYK1Oryza sativa subsp. japonica
D260LYATIDDHHYK1Oryza sativa subsp. japonica
D260IYSTVDDEHYK1Xenopus tropicalis
D260IYPTFDDNNFF1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
D260IYTTIDENHFK1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
D261YSTVDDDHFKS2Homo sapiens, Rattus norvegicus
D261YSTIDEYHFKH1Arabidopsis thaliana
D261YSTLDETCFKS1Arabidopsis thaliana
D261YSTIDESHYKS1Oryza sativa subsp. japonica
D261YATIDDHHYKS1Oryza sativa subsp. japonica
D261YSTVDDEHYKS1Xenopus tropicalis
D261YPTFDDNNFFA1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
D261YTTIDENHFKS1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
D61----VDEVFRI1Homo sapiens
D61-SSGEKSGLVI1Arabidopsis thaliana
D61---TGESNFSI1Arabidopsis thaliana
D61ITGEKGAPFVV1Oryza sativa subsp. japonica
D61-TNDDKSNFAV1Oryza sativa subsp. japonica
D61----NDEMFRI1Xenopus tropicalis
D61----TDEVFKI1Rattus norvegicus
D61--DIQDGPFVS1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
D61--VHADAEFKI1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
E123TVVPPETYVKV2Homo sapiens, Rattus norvegicus
E123ESVRDGTYVRL1Arabidopsis thaliana
E123EAVKLGMYVRL1Arabidopsis thaliana
E123AAVQNGMYVSV1Oryza sativa subsp. japonica
E123ADIQNGDYVIV1Oryza sativa subsp. japonica
E123MVVPPGSYVKV1Xenopus tropicalis
E123QQFEIGGYVKV1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E123SELATDTYVRV1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
E252DFLSNEGHIYS2Homo sapiens, Xenopus tropicalis
E252QSLEGDGLIYS1Arabidopsis thaliana
E252EQLSNDGCIYS1Arabidopsis thaliana
E252DYLADIGHIYS1Oryza sativa subsp. japonica
E252IDQVDLGHLYA1Oryza sativa subsp. japonica
E252DFLCNEGHIYS1Rattus norvegicus
E252TTLTDQGFIYP1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E252DFLQQEGIIYT1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
G253FLSNEGHIYST2Homo sapiens, Xenopus tropicalis
G253SLEGDGLIYST1Arabidopsis thaliana
G253QLSNDGCIYST1Arabidopsis thaliana
G253YLADIGHIYST1Oryza sativa subsp. japonica
G253DQVDLGHLYAT1Oryza sativa subsp. japonica
G253FLCNEGHIYST1Rattus norvegicus
G253TLTDQGFIYPT1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
G253FLQQEGIIYTT1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
H254LSNEGHIYSTV2Homo sapiens, Xenopus tropicalis
H254LEGDGLIYSTI1Arabidopsis thaliana
H254LSNDGCIYSTL1Arabidopsis thaliana
H254LADIGHIYSTI1Oryza sativa subsp. japonica
H254QVDLGHLYATI1Oryza sativa subsp. japonica
H254LCNEGHIYSTV1Rattus norvegicus
H254LTDQGFIYPTF1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
H254LQQEGIIYTTI1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
I255SNEGHIYSTVD2Homo sapiens, Xenopus tropicalis
I255EGDGLIYSTID1Arabidopsis thaliana
I255SNDGCIYSTLD1Arabidopsis thaliana
I255ADIGHIYSTID1Oryza sativa subsp. japonica
I255VDLGHLYATID1Oryza sativa subsp. japonica
I255CNEGHIYSTVD1Rattus norvegicus
I255TDQGFIYPTFD1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
I255QQEGIIYTTID1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L149FKIMPLEDMNE1Homo sapiens
L149FSVRPIMDFNE1Arabidopsis thaliana
L149FSVRPVTDFNE1Arabidopsis thaliana
L149FAIRPVTDYNE1Oryza sativa subsp. japonica
L149YSVRRITNFND1Oryza sativa subsp. japonica
L149FKIAPVDDMNE1Xenopus tropicalis
L149FKIIPLEDMNE1Rattus norvegicus
L149AVIKPIDSFNE1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L149QYIRTIKDHNE1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
M152MPLEDMNEFTT1Homo sapiens
M152RPIMDFNEVTF1Arabidopsis thaliana
M152RPVTDFNEIVH1Arabidopsis thaliana
M152RPVTDYNEVTL1Oryza sativa subsp. japonica
M152RRITNFNDVTH1Oryza sativa subsp. japonica
M152APVDDMNEFVS1Xenopus tropicalis
M152IPLEDMNEFTA1Rattus norvegicus
M152KPIDSFNEVLT1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
M152RTIKDHNEVHF1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
N251VDFLSNEGHIY2Homo sapiens, Xenopus tropicalis
N251VQSLEGDGLIY1Arabidopsis thaliana
N251LEQLSNDGCIY1Arabidopsis thaliana
N251IDYLADIGHIY1Oryza sativa subsp. japonica
N251IIDQVDLGHLY1Oryza sativa subsp. japonica
N251VDFLCNEGHIY1Rattus norvegicus
N251CTTLTDQGFIY1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
N251TDFLQQEGIIY1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
P222-ACPRPE----2Homo sapiens, Rattus norvegicus
P222PACTARQQ---1Arabidopsis thaliana
P222PMHIVSEA---1Arabidopsis thaliana
P222PANVESEH---1Oryza sativa subsp. japonica
P222PAVMNDDH---1Oryza sativa subsp. japonica
P222-SFKGNE----1Xenopus tropicalis
P222-QCEGKDANSF1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
P222-SAPETNE---1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
S250AVDFLSNEGHI2Homo sapiens, Xenopus tropicalis
S250VVQSLEGDGLI1Arabidopsis thaliana
S250ALEQLSNDGCI1Arabidopsis thaliana
S250AIDYLADIGHI1Oryza sativa subsp. japonica
S250IIIDQVDLGHL1Oryza sativa subsp. japonica
S250AVDFLCNEGHI1Rattus norvegicus
S250CCTTLTDQGFI1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
S250VTDFLQQEGII1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
S257EGHIYSTVDDD2Homo sapiens, Rattus norvegicus
S257DGLIYSTIDEY1Arabidopsis thaliana
S257DGCIYSTLDET1Arabidopsis thaliana
S257IGHIYSTIDES1Oryza sativa subsp. japonica
S257LGHLYATIDDH1Oryza sativa subsp. japonica
S257EGHIYSTVDDE1Xenopus tropicalis
S257QGFIYPTFDDN1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
S257EGIIYTTIDEN1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
S266DDHFKSTDAE-2Homo sapiens, Rattus norvegicus
S266EYHFKHVEL--1Arabidopsis thaliana
S266ETCFKSTANA-1Arabidopsis thaliana
S266ESHYKSAFNE-1Oryza sativa subsp. japonica
S266DHHYKSTMNG-1Oryza sativa subsp. japonica
S266DEHYKSTDGD-1Xenopus tropicalis
S266DNNFFAL----1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
S266ENHFKSVLQDQ1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
T110RQWVDTDDT--2Homo sapiens, Rattus norvegicus
T110KRWVSETFD--1Arabidopsis thaliana
T110VRWCHARQE--1Arabidopsis thaliana
T110IRWVNDGAD--1Oryza sativa subsp. japonica
T110NRWENDSTD--1Oryza sativa subsp. japonica
T110RQWVDTDEA--1Xenopus tropicalis
T110RKWSEDANDLA1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
T110RHWEHIDAL--1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
T124VVPPETYVKVA2Homo sapiens, Rattus norvegicus
T124SVRDGTYVRLS1Arabidopsis thaliana
T124AVKLGMYVRLH1Arabidopsis thaliana
T124AVQNGMYVSVI1Oryza sativa subsp. japonica
T124DIQNGDYVIVN1Oryza sativa subsp. japonica
T124VVPPGSYVKVA1Xenopus tropicalis
T124QFEIGGYVKVF1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
T124ELATDTYVRVY1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
T258GHIYSTVDDDH2Homo sapiens, Rattus norvegicus
T258GLIYSTIDEYH1Arabidopsis thaliana
T258GCIYSTLDETC1Arabidopsis thaliana
T258GHIYSTIDESH1Oryza sativa subsp. japonica
T258GHLYATIDDHH1Oryza sativa subsp. japonica
T258GHIYSTVDDEH1Xenopus tropicalis
T258GFIYPTFDDNN1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
T258GIIYTTIDENH1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
T267DHFKSTDAE-2Homo sapiens, Rattus norvegicus
T267YHFKHVEL--1Arabidopsis thaliana
T267TCFKSTANA-1Arabidopsis thaliana
T267SHYKSAFNE-1Oryza sativa subsp. japonica
T267HHYKSTMNG-1Oryza sativa subsp. japonica
T267EHYKSTDGD-1Xenopus tropicalis
T267NNFFAL----1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
T267NHFKSVLQDQ1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
V259HIYSTVDDDHF2Homo sapiens, Rattus norvegicus
V259LIYSTIDEYHF1Arabidopsis thaliana
V259CIYSTLDETCF1Arabidopsis thaliana
V259HIYSTIDESHY1Oryza sativa subsp. japonica
V259HLYATIDDHHY1Oryza sativa subsp. japonica
V259HIYSTVDDEHY1Xenopus tropicalis
V259FIYPTFDDNNF1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
V259IIYTTIDENHF1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Y125VPPETYVKVAG2Homo sapiens, Rattus norvegicus
Y125VRDGTYVRLSG1Arabidopsis thaliana
Y125VKLGMYVRLHG1Arabidopsis thaliana
Y125VQNGMYVSVIG1Oryza sativa subsp. japonica
Y125IQNGDYVIVNG1Oryza sativa subsp. japonica
Y125VPPGSYVKVAG1Xenopus tropicalis
Y125FEIGGYVKVFG1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Y125LATDTYVRVYG1Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Y256NEGHIYSTVDD3Homo sapiens, Xenopus tropicalis, Rattus norvegicus
Y256GDGLIYSTIDE1Arabidopsis thaliana
Y256NDGCIYSTLDE1Arabidopsis thaliana
Y256DIGHIYSTIDE1Oryza sativa subsp. japonica
Y256DLGHLYATIDD1Oryza sativa subsp. japonica
Y256DQGFIYPTFDD1Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Y256QEGIIYTTIDE1Schizosaccharomyces pombe (strain 972 / ATCC 24843)


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