mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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	Gene Summary
	Ligand Binding Site Mutation Information
	Protein Structure Related Information
	Gene Expression and Gene-Gene Network
	Phenotype Information
	Pharmacological Information
	Conservation Information for LBS

Gene summary for UBE2G2

Gene summary

Basic gene Info.	Gene symbol	UBE2G2
	Gene name	ubiquitin-conjugating enzyme E2G 2
	Synonyms	UBC7
	Cytomap	UCSC genome browser: 21q22.3
	Type of gene	protein-coding
	RefGenes	NM_001202489.1, NM_003343.5,NM_182688.2,
	Description	ubiquitin carrier protein G2ubiquitin conjugating enzyme 7ubiquitin conjugating enzyme G2ubiquitin-conjugating enzyme E2 G2ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)ubiquitin-conjugating enzyme E2G 2 (homologous to yeast UBC7)ubiquitin
	Modification date	20141207
dbXrefs	MIM : 603124
	HGNC : HGNC
	Ensembl : ENSG00000184787
	HPRD : 04387
	Vega : OTTHUMG00000089179
Protein	UniProt: P60604 go to UniProt's Cross Reference DB Table
Expression	CleanEX: HS_UBE2G2
	BioGPS: 7327
Pathway	NCI Pathway Interaction Database: UBE2G2
	KEGG: UBE2G2
	REACTOME: UBE2G2
	Pathway Commons: UBE2G2
Context	iHOP: UBE2G2
	ligand binding site mutation search in PubMed: UBE2G2
	UCL Cancer Institute: UBE2G2
Assigned class in mutLBSgeneDB	C: This gene just belongs to mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

GO ID	GO Term	PubMed ID
GO:0044257	cellular protein catabolic process	21628527
GO:0070936	protein K48-linked ubiquitination	20061386

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Ligand binding site mutations for UBE2G2

Cancer type specific mutLBS sorted by frequency

LBS	AAchange of nsSNV	Cancer type	# samples
P21	P21L	COAD	1
E50	E50Q	LUAD	1
L163	L163V	LUSC	1

cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

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Protein structure related information for UBE2G2

Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
(ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)

: nsSNV at non-LBS

: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.

LBS	AAchange of nsSNV	Relative stability change
E50	E50Q	-1.3493216
P21	P21L	-0.86382857
L163	L163V	-0.60430519

(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for UBE2G2 from PDB

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Differential gene expression and gene-gene network for UBE2G2

Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)

* Left PPI network was created from samples with mutations in the LBS of UBE2G2 and the right PPI network was created from samples without mutations in the LBS of UBE2G2. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.

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Phenotype information for UBE2G2

Gene level disease information (DisGeNet)

Disease ID

Disease name

# PubMed

Association type

Mutation level pathogenic information (ClinVar annotation)

Allele ID

AA change

Clinical significance

Origin

Phenotype IDs

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Pharmacological information for UBE2G2

Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Drug information targeting mutLBSgene (Approved drugs only)

Drug status

DrugBank ID

Name

Type

Drug structure

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of UBE2G2 go to BioLip

Ligand ID	Ligand short name	Ligand long name	PDB ID	PDB name	mutLBS
III		Peptide ligand (SER,ALA,ASP,GLU,ARG,GLN,ARG,MET,LEU,VAL,GLN,ARG,LYS,ASP,GLU,LEU,LEU,GLN,GLN,ALA,ARG,LYS,ARG,PHE,LEU,ASN,LYS)	2lxp	A	E50 L163
III		Peptide ligand (TRP,SER,ALA,ASP,GLU,ARG,GLN,ARG,MET,LEU,VAL,GLN,ARG,LYS,ASP,GLU,LEU,LEU,GLN,GLN,ALA,ARG,LYS,ARG,PHE,LEU,ASN,LYS)	3h8k	A	P21 E50 L163

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Conservation information for LBS of UBE2G2

Multiple alignments for P60604 in multiple species

LBS	AA sequence	# species	Species
A26	PEGIVAGPMNE	3	Homo sapiens, Mus musculus, Bos taurus
A26	PPGIVAGPKSE	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
A26	PDGITAGPSNE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
E31	AGPMNEENFFE	3	Homo sapiens, Mus musculus, Bos taurus
E31	AGPKSENNIFI	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E31	AGPSNEDDFFT	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
E38	NFFEWEALIMG	3	Homo sapiens, Mus musculus, Bos taurus
E38	NIFIWDCLIQG	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E38	DFFTWDCLIQG	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
E45	LIMGPEDTCFE	3	Homo sapiens, Mus musculus, Bos taurus
E45	LIQGPPDTPYA	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E45	LIQGPDGTPFE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
E50	EDTCFEFGVFP	3	Homo sapiens, Mus musculus, Bos taurus
E50	PDTPYADGVFN	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
E50	DGTPFEGGLYP	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
F51	DTCFEFGVFPA	3	Homo sapiens, Mus musculus, Bos taurus
F51	DTPYADGVFNA	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
F51	GTPFEGGLYPA	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
G164	VQKSLGL	3	Homo sapiens, Mus musculus, Bos taurus
G164	ILKSLGF	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
G164	ARKTLGL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
G23	LNPPEGIVAGP	3	Homo sapiens, Mus musculus, Bos taurus
G23	KDSPPGIVAGP	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
G23	ENGPDGITAGP	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
G27	EGIVAGPMNEE	3	Homo sapiens, Mus musculus, Bos taurus
G27	PGIVAGPKSEN	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
G27	DGITAGPSNED	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
I24	NPPEGIVAGPM	3	Homo sapiens, Mus musculus, Bos taurus
I24	DSPPGIVAGPK	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
I24	NGPDGITAGPS	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L16	AEYKQLTLNPP	3	Homo sapiens, Mus musculus, Bos taurus
L16	KELQQLIKDSP	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L16	KEYKELTENGP	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L163	IVQKSLGL	3	Homo sapiens, Mus musculus, Bos taurus
L163	SILKSLGF	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L163	LARKTLGL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L40	FEWEALIMGPE	3	Homo sapiens, Mus musculus, Bos taurus
L40	FIWDCLIQGPP	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L40	FTWDCLIQGPD	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L6	MAGTALKRLMA	3	Homo sapiens, Mus musculus, Bos taurus
L6	MSKTAQKRLLK	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L6	SKAMALRRLMK	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
M42	WEALIMGPEDT	3	Homo sapiens, Mus musculus, Bos taurus
M42	WDCLIQGPPDT	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
M42	WDCLIQGPDGT	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
N30	VAGPMNEENFF	3	Homo sapiens, Mus musculus, Bos taurus
N30	VAGPKSENNIF	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
N30	TAGPSNEDDFF	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
P20	QLTLNPPEGIV	3	Homo sapiens, Mus musculus, Bos taurus
P20	QLIKDSPPGIV	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
P20	ELTENGPDGIT	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
P21	LTLNPPEGIVA	3	Homo sapiens, Mus musculus, Bos taurus
P21	LIKDSPPGIVA	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
P21	LTENGPDGITA	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
P28	GIVAGPMNEEN	3	Homo sapiens, Mus musculus, Bos taurus
P28	GIVAGPKSENN	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
P28	GITAGPSNEDD	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Q160	AKQIVQKSLGL	3	Homo sapiens, Mus musculus, Bos taurus
Q160	VKLSILKSLGF	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Q160	VRRLARKTLGL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
S162	QIVQKSLGL	3	Homo sapiens, Mus musculus, Bos taurus
S162	LSILKSLGF	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
S162	RLARKTLGL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
T17	EYKQLTLNPPE	3	Homo sapiens, Mus musculus, Bos taurus
T17	ELQQLIKDSPP	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
T17	EYKELTENGPD	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
V159	IAKQIVQKSLG	2	Homo sapiens, Mus musculus
V159	QVKLSILKSLG	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
V159	VVRRLARKTLG	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
V159	VAKQIVQKSLG	1	Bos taurus
V25	PPEGIVAGPMN	3	Homo sapiens, Mus musculus, Bos taurus
V25	SPPGIVAGPKS	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
V25	GPDGITAGPSN	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
V53	CFEFGVFPAIL	3	Homo sapiens, Mus musculus, Bos taurus
V53	PYADGVFNAKL	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
V53	PFEGGLYPATL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Y13	RLMAEYKQLTL	3	Homo sapiens, Mus musculus, Bos taurus
Y13	RLLKELQQLIK	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Y13	RLMKEYKELTE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)