mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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	Gene Summary
	Ligand Binding Site Mutation Information
	Protein Structure Related Information
	Gene Expression and Gene-Gene Network
	Phenotype Information
	Pharmacological Information
	Conservation Information for LBS

Gene summary for VDR

Gene summary

Basic gene Info.	Gene symbol	VDR
	Gene name	vitamin D (1,25- dihydroxyvitamin D3) receptor
	Synonyms	NR1I1\|PPP1R163
	Cytomap	UCSC genome browser: 12q13.11
	Type of gene	protein-coding
	RefGenes	NM_000376.2, NM_001017535.1,NM_001017536.1,
	Description	1,25-dihydroxyvitamin D3 receptornuclear receptor subfamily 1 group I member 1protein phosphatase 1, regulatory subunit 163vitamin D nuclear receptor variant 1vitamin D3 receptor
	Modification date	20141222
dbXrefs	MIM : 601769
	HGNC : HGNC
	Ensembl : ENSG00000111424
	HPRD : 03463
	Vega : OTTHUMG00000169890
Protein	UniProt: P11473 go to UniProt's Cross Reference DB Table
Expression	CleanEX: HS_VDR
	BioGPS: 7421
Pathway	NCI Pathway Interaction Database: VDR
	KEGG: VDR
	REACTOME: VDR
	Pathway Commons: VDR
Context	iHOP: VDR
	ligand binding site mutation search in PubMed: VDR
	UCL Cancer Institute: VDR
Assigned class in mutLBSgeneDB	A: This gene has a literature evidence and it belongs to targetable_mutLBSgenes.
References showing study about ligand binding site mutation for VDR.	1. "Papadopoulou A, Bountouvi E, Gole E, Doulgeraki A, Tournis S, Papadimitriou A, Nicolaidou P. Identification of a novel nonsense mutation in the ligand-binding domain of the vitamin d receptor gene and clinical description of two greek patients with hereditary vitamin d-resistant rickets and alopecia. Horm Res Paediatr. 2014;82(3):206-12. doi: 10.1159/000362618. Epub 2014 Jul 23." 25060608 2. "Yaghmaei S, Roberts C, Ai R, Mizwicki MT, Chang CE. Agonist and antagonist binding to the nuclear vitamin D receptor: dynamics, mutation effects and functional implications. In Silico Pharmacol. 2013 Feb 12;1:2. doi:10.1186/2193-9616-1-2. eCollection 2013." 25505647 3. "Futawaka K, Tagami T, Fukuda Y, Koyama R, Nushida A, Nezu S, Yamamoto H, Imamoto M, Kasahara M, Moriyama K. Transcriptional activation of the wild-type and mutant vitamin D receptors by vitamin D3. J Mol Endocrinol. 2016 May 6. pii: JME-16-0048." 27154546

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

GO ID	GO Term	PubMed ID
GO:0000122	negative regulation of transcription from RNA polymerase II promoter	17426122
GO:0008285	negative regulation of cell proliferation	16549446
GO:0010980	positive regulation of vitamin D 24-hydroxylase activity	16549446
GO:0038183	bile acid signaling pathway	12016314
GO:0045892	negative regulation of transcription, DNA-templated	11891224
GO:0070561	vitamin D receptor signaling pathway	16549446

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Ligand binding site mutations for VDR

Lollipop-style diagram of mutations at LBS in amino-acid sequence.
We represented ligand binding site mutations only. (You can see big image via clicking.)

: non-synonymous mutation on LBS, Circle size denotes number of samples.

Cancer type specific mutLBS sorted by frequency

LBS	AAchange of nsSNV	Cancer type	# samples
R74	R74H	UCEC	2
V300	D299E	COAD	1
L414	T415A	HNSC	1
C27	G28A	KIRC	1
Y295	K294M	LUAD	1
H397,Y401	K399M	LUSC	1
F150	D149Y	OV	1
A231	A231V	SKCM	1
S278	F279L	SKCM	1
I268	A267V	STAD	1
F47	F47Y	STAD	1
R274	R274C	THCA	1
F34	G33D	UCEC	1
F422,V418	E420K	UCEC	1

cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

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Protein structure related information for VDR

Protein structure of wild type (WT) and mutant type (MT) of VDR

Wild type VDR

Mutant type VDR

Free energy of binding of drugs to wild type and mutant tpye of VDR

Gene symbol	Drug name	Free energy of binding (kcal/mol) of wild type	Free energy of binding (kcal/mol) of mutant type
VDR	Calcidiol	-7.7	-7.9
VDR	Calcipotriol	-8.5	-8.5

Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
(ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)

: nsSNV at non-LBS

: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.

LBS	AAchange of nsSNV	Relative stability change
R74	R74H	-1.4793518
F47	F47Y	-1.2128435
Y295	K294M	-1.1266264
V418	E420K	-1.0958124
F422	E420K	-1.0958124
L414	T415A	-0.84869982
R274	R274C	-0.81537371
S278	F279L	-0.47664603
I268	A267V	-0.44992448
C27	G28A	-0.4192122
A231	A231V	-0.41031066
F150	D149Y	-0.3821139
H397	K399M	-0.15915047
Y401	K399M	-0.15915047
F34	G33D	-0.12455876
V300	D299E	-0.000808632

(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for VDR from PDB

PDB ID	PDB title	PDB structure
3WGP	Crystal Structure Analysis of Vitamin D receptor

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Differential gene expression and gene-gene network for VDR

Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)

* Left PPI network was created from samples with mutations in the LBS of VDR and the right PPI network was created from samples without mutations in the LBS of VDR. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.

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Phenotype information for VDR

Gene level disease information (DisGeNet)

Disease ID	Disease name	# PubMed	Association type
umls:C0029456	Osteoporosis	76	Biomarker, GeneticVariation
umls:C0035579	Rickets	24	Biomarker, GeneticVariation
umls:C0033578	Prostatic Neoplasms	18	AlteredExpression, Biomarker, GeneticVariation
umls:C0042870	Vitamin D Deficiency	15	Biomarker, GeneticVariation
umls:C0342646	Vitamin D-Dependent Rickets, Type 2A	9	Biomarker, GeneticVariation
umls:C0020538	Hypertension	6	Biomarker, GeneticVariation
umls:C0027651	Neoplasms	5	Biomarker, GeneticVariation
umls:C0002170	Alopecia	4	Biomarker, GeneticVariation
umls:C0041948	Uremia	3	Biomarker, GeneticVariation
umls:C0268690	Rickets, Hereditary Vitamin D-Resistant	3	Biomarker, GeneticVariation
umls:C0007138	Carcinoma, Transitional Cell	1	Biomarker
umls:C0014556	Epilepsy, Temporal Lobe	1	Biomarker
umls:C0950123	Genetic Diseases, Inborn	1	Biomarker
umls:C0023434	Leukemia, Lymphocytic, Chronic, B-Cell	1	Biomarker

Mutation level pathogenic information (ClinVar annotation)

Allele ID	AA change	Clinical significance	Origin	Phenotype IDs
22784	G33D	Pathogenic	Germline	MedGen:C0268690 OMIM:277440 Orphanet:ORPHA93160 SNOMED CT:72831007

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Pharmacological information for VDR

Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Gene-centered drug-gene interaction network

Drug information targeting mutLBSgene (Approved drugs only)

Drug status	DrugBank ID	Name	Type	Drug structure
Approved\|nutraceutical	DB00136	Calcitriol	Small molecule
Approved\|nutraceutical	DB00146	Calcidiol	Small molecule
Approved\|nutraceutical	DB00153	Ergocalciferol	Small molecule
Approved\|nutraceutical	DB00169	Cholecalciferol	Small molecule
Approved\|investigational	DB00910	Paricalcitol	Small molecule
Approved	DB01070	Dihydrotachysterol	Small molecule
Approved\|nutraceutical	DB01436	Alfacalcidol	Small molecule
Approved	DB02300	Calcipotriol	Small molecule
Experimental	DB03451	1-alpha, 25-dihydroxyl-20-epi-22-oxa-24, 26 ,27-trihomovitamin D3	Small molecule
Experimental	DB04258	Seocalcitol	Small molecule
Experimental	DB04796	Inecalcitol	Small molecule
Investigational	DB05024	CTA018	Small molecule
Investigational	DB05295	Eldecalcitol	Small molecule
Approved	DB06410	Doxercalciferol	Small molecule
Experimental	DB07530	(1R,3R)-5-[(2E)-3-{(1S,3R)-2,2,3-trimethyl-3-[6,6,6-trifluoro-5-hydroxy-5-(trifluoromethyl)hex-3-yn-1-yl]cyclopentyl}prop-2-en-1-ylidene]cyclohexane-1,3-diol	Small molecule
Experimental	DB08696	5-{2-[1-(1-METHYL-PROPYL)-7A-METHYL-OCTAHYDRO-INDEN-4-YLIDENE]-ETHYLIDENE}-2-METHYLENE-CYCLOHEXANE-1,3-DIOL	Small molecule
Experimental	DB08742	1,3-CYCLOHEXANEDIOL, 4-METHYLENE-5-[(2E)-[(1S,3AS,7AS)-OCTAHYDRO-1-(5-HYDROXY-5-METHYL-1,3-HEXADIYNYL)-7A-METHYL-4H-INDEN-4-YLIDENE]ETHYLIDENE]-, (1R,3S,5Z)	Small molecule

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of VDR go to BioLip

Ligand ID	Ligand short name	Ligand long name	PDB ID	PDB name	mutLBS
TEJ		(23S)-25-DEHYDRO-1ALPHA-HYDROXYVITAMIN D3-26,23-LACTONE	3a2j	A	A231 R274 S278 V300
KH1		1ALPHA,25-DIHYDROXYL-20-EPI-22-OXA-24,26,27-TRIHOMOVITAMIN D3	1ie8	A	A231 R274 S278 V300 H397
ZNE		(1R,3S,5Z)-5-[(2E)-2-{(1R,3AS,7AR)-1-[(1R,5S)-5-HYDROXY-1-METHYL-5-(1,3-THIAZOL-2-YL)PENTYL]-7A-METHYLOCTAHYDRO-4H-INDEN-4-YLIDENE}ETHYLIDENE]-4-METHYLIDENECYCLOHEXANE-1,3-DIOL	3kpz	A	A231 R274 S278 Y295 H397 Y401 L414 V418
EB1		SEOCALCITOL	1s0z	A	A231 R274 S278 Y295 V300 H397 L414 F422
6DS		(4S)-4-HYDROXY-5-[2-METHYL-4-(3-{3-METHYL-4-[(1E)-4,4, 4-TRIFLUORO-3-HYDROXY-3-(TRIFLUOROMETHYL)BUT-1-EN-1- YL]PHENYL}PENTAN-3-YL)PHENOXY]PENTANOIC ACID	3w0c	A	A231 R274 Y295 V300 H397 Y401 L414 V418 F422
ZN		ZINC(2+)	1kb2	A	C27
ZN		ZINC(2+)	1kb4	A	C27
ZN		ZINC(2+)	1kb6	A	C27
ZN		ZINC(2+)	1ynw	A	C27
VHW		METHYL (1S,3E)-3-{(2R)-2-[(1R,3AS,4E,7AR)-4-{(2Z)-2- [(3R,4S,5R)-3,5-DIHYDROXY-4-(3-HYDROXYPROPOXY)-2- METHYLIDENECYCLOHEXYLIDENE]ETHYLIDENE}-7A- METHYLOCTAHYDRO-1H-INDEN-1-YL]PROPYLIDENE}-1-ETHYL-2- OXOCYCLOPENTANECARBOXYLATE (NON-PREFERRED NAME)	3vhw	A	F150 A231 I268 R274 S278 V300 H397 V418 F422
DS4		[3-FLUORO-2'-METHYL-4'-(3-{3-METHYL-4-[(1E)-4,4,4- TRIFLUORO-3-HYDROXY-3-(TRIFLUOROMETHYL)BUT-1-EN-1- YL]PHENYL}PENTAN-3-YL)BIPHENYL-4-YL]ACETIC ACID	3w0y	A	F150 A231 I268 R274 Y295 H397 Y401 L414 V418 F422
TEJ		(23S)-25-DEHYDRO-1ALPHA-HYDROXYVITAMIN D3-26,23-LACTONE	3a2i	A	F150 A231 R274 S278 V300 H397
DS2		{4-[3-(4-{[(2R)-2-HYDROXY-3,3-DIMETHYLBUTYL]OXY}-3-METHYLPHENYL)PENTAN-3-YL]-2-METHYLPHENOXY}ACETIC ACID	3az1	A	F150 A231 R274 S278 Y295 V300 H397 Y401 F422
DS3		5-{4-[3-(4-{[(2R)-2-HYDROXY-3,3-DIMETHYLBUTYL]OXY}-3-METHYLPHENYL)PENTAN-3-YL]-2-METHYLPHENOXY}PENTANOICACID	3az2	A	F150 A231 R274 S278 Y295 V300 H397 Y401 L414 F422
DS6		(4S)-4-HYDROXY-5-[4-(3-{4-[(3S)-3-HYDROXY-4,4-DIMETHYLPENTYL]-3-METHYLPHENYL}PENTAN-3-YL)-2-METHYLPHENOXY]PENTANOIC ACID	3az3	A	F150 A231 R274 V300 Y401 L414 F422
DS5		(4S)-4-HYDROXY-5-[2-METHYL-4-(3-{3-METHYL-4-[4,4,4- TRIFLUORO-3-HYDROXY-3-(TRIFLUOROMETHYL)BUT-1-YN-1- YL]PHENYL}PENTAN-3-YL)PHENOXY]PENTANOIC ACID	3w0a	A	F150 A231 R274 Y295 V300 H397 Y401 L414 V418 F422
CA9		(1R,2S,3R)-5-[2-[(1R,3AS,7AR)-1-[(2R)-6-HYDROXY-6-METHYL-HEPTAN-2-YL]-7A-METHYL-1,2,3,3A,6,7-HEXAHYDROINDEN-4-YL]ETHYNYL]-2-METHYL-CYCLOHEX-4-ENE-1,3-DIOL	3auq	A	F150 I268 R274 S278 V300 H397 V418
2MV		(1S,2S,3R,5Z,7E,14BETA,17ALPHA)-17-[(2S,4S)-4-(2-HYDROXY-2-METHYLPROPYL)-2-METHYLTETRAHYDROFURAN-2-YL]-2-METHYL-9,10-SECOANDROSTA-5,7,10-TRIENE-1,3-DIOL	3a3z	X	F150 R274 S278 H397
MVD		9,10-SECOCHOLESTA-5,7,10(19)-TRIENE-1,3,25-TRIOL,2-METHYL-,(1A,2A,3B,5Z,7E)	2hb8	A	F150 R274 S278 V300 H397
CA9		(1R,2S,3R)-5-[2-[(1R,3AS,7AR)-1-[(2R)-6-HYDROXY-6-METHYL-HEPTAN-2-YL]-7A-METHYL-1,2,3,3A,6,7-HEXAHYDROINDEN-4-YL]ETHYNYL]-2-METHYL-CYCLOHEX-4-ENE-1,3-DIOL	3aur	A	F150 R274 S278 V300 H397
OCC		9,10-SECOCHOLESTA-5,7,10(19)-TRIENE-1,3,25-TRIOL,2-(3-HYDROXYPROPOXY)-,(1A,2A,3B,5Z,7E)	2har	A	F150 R274 S278 V300 H397 V418
C3O		9,10-SECOCHOLESTA-5,7,10(19)-TRIENE-1,3,25-TRIOL,2-PROPOXY-,(1A,2A,3B,5Z,7E)	2has	A	F150 R274 S278 V300 H397 V418
TEY		(1R,2S,3S,5Z)-5-[(2E)-2-[(1R,3AS,7AR)-7A-METHYL-1- [(2R)-6-METHYL-6-OXIDANYL-HEPTAN-2-YL]-2,3,3A,5,6,7- HEXAHYDRO-1H-INDEN-4-YLIDENE]ETHYLIDENE]-4- METHYLIDENE-2-[2-(1,2,3,4-TETRAZOL-2-YL) ETHYL]CYCLOHEXANE-1,3-DIOL	4ite	A	F150 R274 S278 V300 H397 V418
2KB		4-{[(1R,2S,3R,5Z,7E,14BETA,17ALPHA)-1,3,25-TRIHYDROXY- 9,10-SECOCHOLESTA-5,7,10-TRIEN-2-YL]OXY}BUTANENITRILE	4pa2	A	F150 R274 S278 V300 H397 Y401
23R		(1S,2S,3R,5Z,7E,14BETA,17ALPHA,23R)-23-(2-HYDROXY-2-METHYLPROPYL)-2-METHYL-20,24-EPOXY-9,10-SECOCHOLA-5,7,10-TRIENE-1,3-DIOL	3a40	X	F150 R274 S278 Y295 V300 H397
C33		9,10-SECOCHOLESTA-5,7,10(19)-TRIENE-1,3,25-TRIOL,2-PROPYL-,(1A,2A,3B,5Z,7E)	2ham	A	F150 R274 S278 Y295 V300 H397 V418
O1C		9,10-SECOCHOLESTA-5,7,10(19)-TRIENE-1,3,25-TRIOL,2-(3-HYDROXYPROPYL)-,(1A,2A,3B,5Z,7E)	2hb7	A	F150 R274 S278 Y295 V300 H397 V418
NUC		Nucleic Acids	1kb2	A	F34
NUC		Nucleic Acids	1kb4	A	F34
NUC		Nucleic Acids	1kb6	A	F34
NUC		Nucleic Acids	1ynw	A	F34
NUC		Nucleic Acids	1ynw	A	F47
NUC		Nucleic Acids	1kb2	A	F47 R74
NUC		Nucleic Acids	1kb4	A	F47 R74
NUC		Nucleic Acids	1kb6	A	F47 R74
FMV		(1S,3R,5Z,7E,14BETA,17ALPHA,20S)-20-[5-(1-HYDROXY-1-METHYLETHYL)FURAN-2-YL]-9,10-SECOPREGNA-5,7,10-TRIENE-1,3-DIOL	3ogt	A	I268 R274 S278 V300 H397
FMV		(1S,3R,5Z,7E,14BETA,17ALPHA,20S)-20-[5-(1-HYDROXY-1-METHYLETHYL)FURAN-2-YL]-9,10-SECOPREGNA-5,7,10-TRIENE-1,3-DIOL	3tkc	A	I268 R274 S278 V300 H397
TX5		TX522	1txi	A	I268 R274 S278 Y295 V300 H397
NUC		Nucleic Acids	1kb4	B	R274
NUC		Nucleic Acids	1kb6	B	R274
VDX		CALCITRIOL	1db1	A	R274 S278 H397
3AJ		1-({[(1R,2S,3R,5Z,7E,14BETA,17ALPHA)-1,3,25-TRIHYDROXY- 9,10-SECOCHOLESTA-5,7,10-TRIEN-2-YL]OXY}METHYL) CYCLOPROPANECARBONITRILE	3wwr	A	R274 S278 H397
VDX		CALCITRIOL	1ie9	A	R274 S278 V300 H397
3EV		1ALPHA,25-DIHYDROXY-3-EPI-VITAMIN D3	3a78	A	R274 S278 V300 H397
MCZ		MAXACALCITOL	3b0t	A	R274 S278 V300 H397
COV		(1S,3R,5Z,7E,14BETA,17ALPHA)-17-[(2S,4S)-4-(2-HYDROXY-2-METHYLPROPYL)-2-METHYLTETRAHYDROFURAN-2-YL]-9,10-SECOANDROSTA-5,7,10-TRIENE-1,3-DIOL	3cs4	A	R274 S278 V300 H397
41V		(1R,3R,7Z,14BETA,17ALPHA)-17-[(2R)-6-HYDROXY-6- METHYLHEPTAN-2-YL]-9,10-SECOESTRA-5,7-DIENE-1,3-DIOL	3x31	A	R274 S278 V300 H397
ZYD		(1R,3S,5Z)-5-{(2E)-2-[(3AR,7AS)-7A-(7-HYDROXY-7-METHYLOCTYL)OCTAHYDRO-4H-INDEN-4-YLIDENE]ETHYLIDENE}-4-METHYLIDENECYCLOHEXANE-1,3-DIOL	3p8x	A	R274 S278 V300 H397 L414 V418 F422
EIM		(1R,3S,5Z)-5-[(2E)-2-[(1R,3S,3AS,7AR)-1-[(2R)-6-HYDROXY-6-METHYL-HEPTAN-2-YL]-3-METHOXY-7A-METHYL-2,3,3A,5,6,7-HEXAHYDRO-1H-INDEN-4-YLIDENE]ETHYLIDENE]-4-METHYLIDENE-CYCLOHEXANE-1,3-DIOL	3ax8	A	R274 S278 V300 H397 V418
VDX		CALCITRIOL	3m7r	A	R274 S278 V300 H397 V418
TFY		(1R,2S,3S,5Z)-5-[(2E)-2-[(1R,3AS,7AR)-7A-METHYL-1- [(2R)-6-METHYL-6-OXIDANYL-HEPTAN-2-YL]-2,3,3A,5,6,7- HEXAHYDRO-1H-INDEN-4-YLIDENE]ETHYLIDENE]-4- METHYLIDENE-2-[2-(1,2,3,4-TETRAZOL-1-YL) ETHYL]CYCLOHEXANE-1,3-DIOL	4itf	A	R274 S278 V300 H397 V418
ED9		(1R,2R,3R,5Z,7E,14BETA,17ALPHA)-2-(3-HYDROXYPROPOXY)-9, 10-SECOCHOLESTA-5,7,10-TRIENE-1,3,25-TRIOL	3wgp	A	R274 S278 V300 H397 V418
0VQ		(3E,5E)-6-(3-{2-[3,4-BIS(HYDROXYMETHYL) PHENYL]ETHYL}PHENYL)-1,1,1-TRIFLUORO-2- (TRIFLUOROMETHYL)OCTA-3,5-DIEN-2-OL	4g2i	A	R274 S278 V300 H397 Y401 V418 F422
0CO		(1S,3R,5Z,7E,14BETA,17ALPHA,23R)-23-(2-HYDROXY-2-METHYLPROPYL)-20,24-EPOXY-9,10-SECOCHOLA-5,7,10-TRIENE-1,3-DIOL	3cs6	A	R274 S278 Y295 H397
MC9		CALCIPOTRIOL	1s19	A	R274 S278 Y295 V300 H397
NUC		Nucleic Acids	1kb4	B	V300
NUC		Nucleic Acids	1kb6	B	V300

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Conservation information for LBS of VDR

Multiple alignments for P11473 in multiple species

LBS	AA sequence	# species	Species
A231	MLPHLADLVSY	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
A303	NDVTKAGHTLE	2	Danio rerio, Danio rerio
A303	SDVTKAGHSLE	1	Homo sapiens
A303	SDVTQAGHSMD	1	Gallus gallus
A303	TDVSRAGHTLE	1	Mus musculus
A303	TDVSKAGHTLE	1	Rattus norvegicus
A303	SDVTRAGHSLE	1	Bos taurus
C24	NVPRICGVCGD	6	Homo sapiens, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
C24	NAPRICGVCGD	1	Danio rerio
C27	RICGVCGDRAT	5	Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
C27	RICGVCGDKAT	2	Danio rerio, Danio rerio
C288	DMSWDCGSQDY	2	Mus musculus, Rattus norvegicus
C288	DMSWTCGNQDY	1	Homo sapiens
C288	DMSWSCGGPDF	1	Danio rerio
C288	DMSWSCGGPEF	1	Danio rerio
C288	DMSWTCGSNDF	1	Gallus gallus
C288	DMSWTCGSPDY	1	Bos taurus
C41	FNAMTCEGCKG	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
C44	MTCEGCKGFFR	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
D144	HHKTYDDSYSD	2	Danio rerio, Danio rerio
D144	HHKTYDPTYAD	2	Mus musculus, Rattus norvegicus
D144	HHKTYDPTYSD	1	Homo sapiens
D144	HHKTFDTTYSD	1	Gallus gallus
D144	HHKTYDDTYSD	1	Bos taurus
E42	NAMTCEGCKGF	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
F150	PTYADFRDFRP	2	Mus musculus, Rattus norvegicus
F150	PTYSDFCQFRP	1	Homo sapiens
F150	DSYSDFVRFRP	1	Danio rerio
F150	DSYSDFSRFRP	1	Danio rerio
F150	TTYSDFNKFRP	1	Gallus gallus
F150	DTYSDFSQFRP	1	Bos taurus
F34	DRATGFHFNAM	5	Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
F34	DKATGFHFNAM	2	Danio rerio, Danio rerio
F36	ATGFHFNAMTC	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
F422	LVLEVFGNEIS	4	Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus
F422	LVLEVFGSEVS	1	Danio rerio
F422	LVLEVFGGQVT	1	Danio rerio
F422	LLFEVFGNEIS	1	Bos taurus
F47	EGCKGFFRRSM	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
F93	GMMKEFILTDE	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
G43	AMTCEGCKGFF	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
H305	VTKAGHTLELL	2	Danio rerio, Danio rerio
H305	VTKAGHSLELI	1	Homo sapiens
H305	VTQAGHSMDLL	1	Gallus gallus
H305	VSRAGHTLELI	1	Mus musculus
H305	VSKAGHTLELI	1	Rattus norvegicus
H305	VTRAGHSLELI	1	Bos taurus
H35	RATGFHFNAMT	5	Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
H35	KATGFHFNAMT	2	Danio rerio, Danio rerio
H397	SLNEEHSKQYR	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
H75	KDNRRHCQACR	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
I107	RKREMILKRKE	3	Homo sapiens, Gallus gallus, Bos taurus
I107	RKREMIMKRKE	2	Mus musculus, Rattus norvegicus
I107	RKKDLIMKRKE	1	Danio rerio
I107	RKKELIQRRKD	1	Danio rerio
I268	LKSSAIEVIML	6	Homo sapiens, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
I268	LKSSAIEIIML	1	Danio rerio
I271	SAIEVIMLRSN	6	Homo sapiens, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
I271	SAIEIIMLRSN	1	Danio rerio
I94	MMKEFILTDEE	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
K240	SYSIQKVIGFA	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
K45	TCEGCKGFFRR	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
L227	SQLSMLPHLAD	2	Homo sapiens, Bos taurus
L227	SPLSMLPHLAD	2	Mus musculus, Rattus norvegicus
L227	SRLSMLPHLAD	1	Danio rerio
L227	SSLSMLPHLAD	1	Danio rerio
L227	PHLPMLPHLAD	1	Gallus gallus
L230	SMLPHLADLVS	6	Homo sapiens, Danio rerio, Danio rerio, Mus musculus, Rattus norvegicus, Bos taurus
L230	PMLPHLADLVS	1	Gallus gallus
L233	PHLADLVSYSI	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
L309	GHSLELIEPLI	2	Homo sapiens, Bos taurus
L309	GHTLELLEPLV	2	Danio rerio, Danio rerio
L309	GHTLELIEPLI	2	Mus musculus, Rattus norvegicus
L309	GHSMDLLEPLV	1	Gallus gallus
L313	ELIEPLIKFQV	4	Homo sapiens, Mus musculus, Rattus norvegicus, Bos taurus
L313	ELLEPLVKFQV	2	Danio rerio, Danio rerio
L313	DLLEPLVKFQV	1	Gallus gallus
L404	KQYRSLSFQPE	4	Danio rerio, Danio rerio, Mus musculus, Rattus norvegicus
L404	KQYRCLSFQPE	3	Homo sapiens, Gallus gallus, Bos taurus
L414	EHSMQLTPLVL	3	Danio rerio, Danio rerio, Gallus gallus
L414	ENSMKLTPLVL	2	Mus musculus, Rattus norvegicus
L414	ECSMKLTPLVL	1	Homo sapiens
L414	ESSMKLTPLLF	1	Bos taurus
L95	MKEFILTDEEV	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
M272	AIEVIMLRSNQ	5	Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
M272	AIEVIMLRSNE	1	Homo sapiens
M272	AIEIIMLRSNQ	1	Danio rerio
Q77	NRRHCQACRLK	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
R104	EVQRKREMILK	3	Homo sapiens, Gallus gallus, Bos taurus
R104	EVQRKREMIMK	2	Mus musculus, Rattus norvegicus
R104	EVQRKKDLIMK	1	Danio rerio
R104	EVQRKKELIQR	1	Danio rerio
R274	EVIMLRSNQSF	5	Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
R274	EVIMLRSNESF	1	Homo sapiens
R274	EIIMLRSNQSF	1	Danio rerio
R49	CKGFFRRSMKR	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
R50	KGFFRRSMKRK	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
R54	RRSMKRKALFT	4	Homo sapiens, Mus musculus, Rattus norvegicus, Bos taurus
R54	RRSMKRKASFT	2	Danio rerio, Danio rerio
R54	RRSMKRKAMFT	1	Gallus gallus
R73	ITKDNRRHCQA	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
R74	TKDNRRHCQAC	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
R80	HCQACRLKRCV	4	Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus
R80	HCQACRLKRCI	2	Danio rerio, Bos taurus
R80	HCQACRLKRCL	1	Danio rerio
S237	DLVSYSIQKVI	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
S275	VIMLRSNQSFT	4	Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
S275	VIMLRSNESFT	1	Homo sapiens
S275	IIMLRSNQSFS	1	Danio rerio
S275	VIMLRSNQSFS	1	Danio rerio
S278	LRSNQSFSL-E	2	Danio rerio, Danio rerio
S278	LRSNESFTM-D	1	Homo sapiens
S278	LRSNQSFTM-E	1	Gallus gallus
S278	LRSNQSFTL-D	1	Mus musculus
S278	LRSNQSFTM-D	1	Rattus norvegicus
S278	LRSNQSFTLDD	1	Bos taurus
T142	DAHHKTYDPTY	3	Homo sapiens, Mus musculus, Rattus norvegicus
T142	EAHHKTYDDSY	1	Danio rerio
T142	DAHHKTYDDSY	1	Danio rerio
T142	EAHHKTFDTTY	1	Gallus gallus
T142	EAHHKTYDDTY	1	Bos taurus
V100	LTDEEVQRKRE	5	Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
V100	LTDEEVQRKKD	1	Danio rerio
V100	LTDEEVQRKKE	1	Danio rerio
V234	HLADLVSYSIQ	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
V300	YRVSDVTKAGH	1	Homo sapiens
V300	YCINDVTKAGH	1	Danio rerio
V300	YCVNDVTKAGH	1	Danio rerio
V300	YKVSDVTQAGH	1	Gallus gallus
V300	YDITDVSRAGH	1	Mus musculus
V300	YDVTDVSKAGH	1	Rattus norvegicus
V300	YQVSDVTRAGH	1	Bos taurus
V418	KLTPLVLEVFG	3	Homo sapiens, Mus musculus, Rattus norvegicus
V418	QLTPLVLEVFG	3	Danio rerio, Danio rerio, Gallus gallus
V418	KLTPLLFEVFG	1	Bos taurus
W286	-EDMSWSCGGP	2	Danio rerio, Danio rerio
W286	-DDMSWDCGSQ	2	Mus musculus, Rattus norvegicus
W286	-DDMSWTCGNQ	1	Homo sapiens
W286	-EDMSWTCGSN	1	Gallus gallus
W286	DDDMSWTCGSP	1	Bos taurus
Y143	AHHKTYDDSYS	2	Danio rerio, Danio rerio
Y143	AHHKTYDPTYA	2	Mus musculus, Rattus norvegicus
Y143	AHHKTYDPTYS	1	Homo sapiens
Y143	AHHKTFDTTYS	1	Gallus gallus
Y143	AHHKTYDDTYS	1	Bos taurus
Y147	TYDPTYADFRD	2	Mus musculus, Rattus norvegicus
Y147	TYDPTYSDFCQ	1	Homo sapiens
Y147	TYDDSYSDFVR	1	Danio rerio
Y147	TYDDSYSDFSR	1	Danio rerio
Y147	TFDTTYSDFNK	1	Gallus gallus
Y147	TYDDTYSDFSQ	1	Bos taurus
Y236	ADLVSYSIQKV	7	Homo sapiens, Danio rerio, Danio rerio, Gallus gallus, Mus musculus, Rattus norvegicus, Bos taurus
Y295	NQDYKYRVSDV	1	Homo sapiens
Y295	GPDFKYCINDV	1	Danio rerio
Y295	GPEFKYCVNDV	1	Danio rerio
Y295	SNDFKYKVSDV	1	Gallus gallus
Y295	SQDYKYDITDV	1	Mus musculus
Y295	SQDYKYDVTDV	1	Rattus norvegicus
Y295	SPDYKYQVSDV	1	Bos taurus
Y401	EHSKQYRSLSF	4	Danio rerio, Danio rerio, Mus musculus, Rattus norvegicus
Y401	EHSKQYRCLSF	3	Homo sapiens, Gallus gallus, Bos taurus