mutated Ligand Binding Site gene DataBase





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Gene Summary

Ligand Binding Site Mutation Information

Protein Structure Related Information

Gene Expression and Gene-Gene Network

Phenotype Information

Pharmacological Information

Conservation Information for LBS

Gene summary for WEE1
Gene summary
Basic gene Info.Gene symbolWEE1
Gene nameWEE1 G2 checkpoint kinase
CytomapUCSC genome browser: 11p15.4
Type of geneprotein-coding
DescriptionWEE1 homologWEE1+ homologwee1-like protein kinasewee1A kinase
Modification date20141222
dbXrefs MIM : 193525
Ensembl : ENSG00000166483
HPRD : 01907
Vega : OTTHUMG00000165863
ProteinUniProt: P30291
go to UniProt's Cross Reference DB Table
ExpressionCleanEX: HS_WEE1
BioGPS: 7465
PathwayNCI Pathway Interaction Database: WEE1
Pathway Commons: WEE1
ContextiHOP: WEE1
ligand binding site mutation search in PubMed: WEE1
UCL Cancer Institute: WEE1
Assigned class in mutLBSgeneDBB: This gene belongs to targetable_mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

Ligand binding site mutations for WEE1
Lollipop-style diagram of mutations at LBS in amino-acid sequence.
We represented ligand binding site mutations only. (You can see big image via clicking.)
: non-synonymous mutation on LBS, Circle size denotes number of samples.

Cancer type specific mutLBS sorted by frequency
LBSAAchange of nsSNVCancer type# samples
cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

Protein structure related information for WEE1
Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
  (ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)
: nsSNV at non-LBS: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.
LBSAAchange of nsSNVRelative stability change
(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for WEE1 from PDB
PDB IDPDB titlePDB structure
3CQEWee1 kinase complex with inhibitor PD074291

Differential gene expression and gene-gene network for WEE1
Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)
* Left PPI network was created from samples with mutations in the LBS of WEE1 and the right PPI network was created from samples without mutations in the LBS of WEE1. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.


Phenotype information for WEE1
Gene level disease information (DisGeNet)
Disease IDDisease name# PubMedAssociation type

Mutation level pathogenic information (ClinVar annotation)
Allele IDAA changeClinical significanceOriginPhenotype IDs

Pharmacological information for WEE1
Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Gene-centered drug-gene interaction network
Drug information targeting mutLBSgene (Approved drugs only)
Drug statusDrugBank IDNameTypeDrug structure
ExperimentalDB046089-HYDROXY-4-PHENYL-6H-PYRROLO[3,4-C]CARBAZOLE-1,3-DIONESmall molecule
ExperimentalDB070069-HYDROXY-6-(3-HYDROXYPROPYL)-4-(2-METHOXYPHENYL)PYRROLO[3,4-C]CARBAZOLE-1,3(2H,6H)-DIONESmall molecule
ExperimentalDB07226N-[4-(2-CHLOROPHENYL)-1,3-DIOXO-1,2,3,6-TETRAHYDROPYRROLO[3,4-C]CARBAZOL-9-YL]FORMAMIDESmall molecule
ExperimentalDB072574-(2-chlorophenyl)-8-(2-hydroxyethyl)-6-methylpyrrolo[3,4-e]indole-1,3(2H,6H)-dioneSmall molecule
ExperimentalDB072653-(9-HYDROXY-1,3-DIOXO-4-PHENYL-2,3-DIHYDROPYRROLO[3,4-C]CARBAZOL-6(1H)-YL)PROPANOIC ACIDSmall molecule
ExperimentalDB083658-bromo-4-(2-chlorophenyl)-N-(2-hydroxyethyl)-6-methyl-1,3-dioxo-1,2,3,6-tetrahydropyrrolo[3,4-e]indole-7-carboxamideSmall molecule

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of WEE1 go to BioLip
Ligand IDLigand short nameLigand long namePDB IDPDB namemutLBS

Conservation information for LBS of WEE1
Multiple alignments for P30291 in multiple species
LBSAA sequence# speciesSpecies
A326DGCIYAIKRSK3Homo sapiens, Mus musculus, Rattus norvegicus
A326DGCIYAIKKSK1Drosophila melanogaster
C379IQNEYCNGGSL3Homo sapiens, Mus musculus, Rattus norvegicus
C379IQNEFCDGGSL1Drosophila melanogaster
D386GGSLADAISEN2Homo sapiens, Mus musculus
D386GGSLHARIQDH1Drosophila melanogaster
D386GGSLADAVSEN1Rattus norvegicus
D463MFKIGDLGHVT3Homo sapiens, Mus musculus, Rattus norvegicus
D463TYKIGDLGHVT1Drosophila melanogaster
E303EFHELEKIGSG3Homo sapiens, Mus musculus, Rattus norvegicus
E303EFMQVNVIGVG1Drosophila melanogaster
E346QNALREVYAHA3Homo sapiens, Mus musculus, Rattus norvegicus
E346KRALNEVWAHA1Drosophila melanogaster
E377MLIQNEYCNGG3Homo sapiens, Mus musculus, Rattus norvegicus
E377MLIQNEFCDGG1Drosophila melanogaster
G306ELEKIGSGEFG3Homo sapiens, Mus musculus, Rattus norvegicus
G306QVNVIGVGEFG1Drosophila melanogaster
G381NEYCNGGSLAD3Homo sapiens, Mus musculus, Rattus norvegicus
G381NEFCDGGSLHA1Drosophila melanogaster
G382EYCNGGSLADA3Homo sapiens, Mus musculus, Rattus norvegicus
G382EFCDGGSLHAR1Drosophila melanogaster
G465KIGDLGHVTRI3Homo sapiens, Mus musculus, Rattus norvegicus
G465KIGDLGHVTSV1Drosophila melanogaster
I305HELEKIGSGEF3Homo sapiens, Mus musculus, Rattus norvegicus
I305MQVNVIGVGEF1Drosophila melanogaster
I374DDHMLIQNEYC3Homo sapiens, Mus musculus, Rattus norvegicus
I374DDHMLIQNEFC1Drosophila melanogaster
K328CIYAIKRSKKP3Homo sapiens, Mus musculus, Rattus norvegicus
K328CIYAIKKSKKP1Drosophila melanogaster
N342SVDEQNALREV3Homo sapiens, Mus musculus, Rattus norvegicus
N342SSFEKRALNEV1Drosophila melanogaster
N376HMLIQNEYCNG3Homo sapiens, Mus musculus, Rattus norvegicus
N376HMLIQNEFCDG1Drosophila melanogaster
N380QNEYCNGGSLA3Homo sapiens, Mus musculus, Rattus norvegicus
N380QNEFCDGGSLH1Drosophila melanogaster
N431DIKPSNIFISR3Homo sapiens, Mus musculus, Rattus norvegicus
N431DLKPENIFSTM1Drosophila melanogaster
R345EQNALREVYAH3Homo sapiens, Mus musculus, Rattus norvegicus
R345EKRALNEVWAH1Drosophila melanogaster
S383YCNGGSLADAI2Homo sapiens, Mus musculus
S383FCDGGSLHARI1Drosophila melanogaster
S383YCNGGSLADAV1Rattus norvegicus
S430MDIKPSNIFIS3Homo sapiens, Mus musculus, Rattus norvegicus
S430MDLKPENIFST1Drosophila melanogaster
V313GEFGSVFKCVK3Homo sapiens, Mus musculus, Rattus norvegicus
V313GEFGVVFQCVN1Drosophila melanogaster
V360QHPHVVRYFSA2Mus musculus, Rattus norvegicus
V360QHSHVVRYFSA1Homo sapiens
V360KHDNVVRYYSA1Drosophila melanogaster
Y378LIQNEYCNGGS3Homo sapiens, Mus musculus, Rattus norvegicus
Y378LIQNEFCDGGS1Drosophila melanogaster

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