mutLBSgeneDB

mutLBSgeneDB
mutated Ligand Binding Site gene DataBase

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	Gene Summary
	Ligand Binding Site Mutation Information
	Protein Structure Related Information
	Gene Expression and Gene-Gene Network
	Phenotype Information
	Pharmacological Information
	Conservation Information for LBS

Gene summary for AURKB

Gene summary

Basic gene Info.	Gene symbol	AURKB
	Gene name	aurora kinase B
	Synonyms	AIK2\|AIM-1\|AIM1\|ARK2\|AurB\|IPL1\|PPP1R48\|STK12\|STK5\|aurkb-sv1\|aurkb-sv2
	Cytomap	UCSC genome browser: 17p13.1
	Type of gene	protein-coding
	RefGenes	NM_001256834.1, NM_001284526.1,NM_004217.3,
	Description	ARK-2STK-1aurora 1aurora kinase B-Sv1aurora kinase B-Sv2aurora- and Ipl1-like midbody-associated protein 1aurora-1aurora-Baurora-related kinase 2aurora/IPL1-related kinase 2protein phosphatase 1, regulatory subunit 48serine/threonine kinase 12
	Modification date	20141222
dbXrefs	MIM : 604970
	HGNC : HGNC
	Ensembl : ENSG00000178999
	HPRD : 05397
	Vega : OTTHUMG00000108189
Protein	UniProt: Q96GD4 go to UniProt's Cross Reference DB Table
Expression	CleanEX: HS_AURKB
	BioGPS: 9212
Pathway	NCI Pathway Interaction Database: AURKB
	KEGG: AURKB
	REACTOME: AURKB
	Pathway Commons: AURKB
Context	iHOP: AURKB
	ligand binding site mutation search in PubMed: AURKB
	UCL Cancer Institute: AURKB
Assigned class in mutLBSgeneDB	B: This gene belongs to targetable_mutLBSgenes.

Gene ontology having evidence of Inferred from Direct Assay (IDA) from Entrez

GO ID	GO Term	PubMed ID
GO:0000122	negative regulation of transcription from RNA polymerase II promoter	20959462
GO:0002903	negative regulation of B cell apoptotic process	20959462
GO:0006468	protein phosphorylation	21820309
GO:0032091	negative regulation of protein binding	21820309
GO:0034644	cellular response to UV	20959462
GO:0036089	cleavage furrow formation	16103226

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Ligand binding site mutations for AURKB

Lollipop-style diagram of mutations at LBS in amino-acid sequence.
We represented ligand binding site mutations only. (You can see big image via clicking.)

: non-synonymous mutation on LBS, Circle size denotes number of samples.

Cancer type specific mutLBS sorted by frequency

LBS	AAchange of nsSNV	Cancer type	# samples
P158	P158A	KIRC	1
A104	A104S	LUAD	1

cf) Cancer type abbreviation. BLCA: Bladder urothelial carcinoma, BRCA: Breast invasive carcinoma, CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma, COAD: Colon adenocarcinoma, GBM: Glioblastoma multiforme, LGG: Brain lower grade glioma, HNSC: Head and neck squamous cell carcinoma, KICH: Kidney chromophobe, KIRC: Kidney renal clear cell carcinoma, KIRP: Kidney renal papillary cell carcinoma, LAML: Acute myeloid leukemia, LUAD: Lung adenocarcinoma, LUSC: Lung squamous cell carcinoma, OV: Ovarian serous cystadenocarcinoma, PAAD: Pancreatic adenocarcinoma, PRAD: Prostate adenocarcinoma, SKCM: Skin cutaneous melanoma, STAD: Stomach adenocarcinoma, THCA: Thyroid carcinoma, UCEC: Uterine corpus endometrial carcinoma.

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Protein structure related information for AURKB

Relative protein structure stability change (ΔΔE) using Mupro 1.1
Mupro score denotes assessment of the effect of mutations on thermodynamic stability.
(ΔΔE<0: mutation decreases stability, ΔΔE>0: mutation increases stability)

: nsSNV at non-LBS

: nsSNV at LBS

nsSNVs sorted by the relative stability change of protein structure by each mutation
Blue: mutations of positive stability change. and red : the most recurrent mutation for this gene.

LBS	AAchange of nsSNV	Relative stability change
P158	P158A	-1.3550979
A104	A104S	-1.0168868

(MuPro1.1: Jianlin Cheng et al., Prediction of Protein Stability Changes for Single-Site Mutations Using Support Vector Machines, PROTEINS: Structure, Function, and Bioinformatics. 2006, 62:1125-1132)

Structure image for AURKB from PDB

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Differential gene expression and gene-gene network for AURKB

Differential gene expression between mutated and non-mutated LBS samples in all 16 major cancer types

Differential co-expressed gene network based on protein-protein interaction data (CePIN)

* Left PPI network was created from samples with mutations in the LBS of AURKB and the right PPI network was created from samples without mutations in the LBS of AURKB. Only genes with p-value < 0.05 are shown.
Red circle: input gene. Orange circle: LBSgene. Blue circle: other gene.

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Phenotype information for AURKB

Gene level disease information (DisGeNet)

Disease ID	Disease name	# PubMed	Association type
umls:C0017661	Glomerulonephritis, IGA	1	Biomarker
umls:C0038356	Stomach Neoplasms	1	Biomarker

Mutation level pathogenic information (ClinVar annotation)

Allele ID

AA change

Clinical significance

Origin

Phenotype IDs

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Pharmacological information for AURKB

Gene expression profile of anticancer drug treated cell-lines (CCLE)
Heatmap showing the correlation between gene expression and drug response across all the cell-lines. We chose the top 20 among 138 drugs.We used Pearson's correlation coefficient.

Gene-centered drug-gene interaction network

Drug information targeting mutLBSgene (Approved drugs only)

Drug status	DrugBank ID	Name	Type	Drug structure
Experimental	DB04703	HESPERIDIN	Small molecule
Investigational	DB05169	AT9283	Small molecule
Experimental	DB07340	N~6~-cyclohexyl-N~2~-(4-morpholin-4-ylphenyl)-9H-purine-2,6-diamine	Small molecule

Gene-centered ligand-gene interaction network

Ligands binding to mutated ligand binding site of AURKB go to BioLip

Ligand ID	Ligand short name	Ligand long name	PDB ID	PDB name	mutLBS
VX6		CYCLOPROPANECARBOXYLIC ACID {4-[4-(4-METHYL-PIPERAZIN-1-YL)-6-(5-METHYL-2H-PYRAZOL-3-YLAMINO)-PYRIMIDIN-2-YLSULFANYL]-PHENYL}-AMIDE	4af3	A	A104 P158

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Conservation information for LBS of AURKB

Multiple alignments for Q96GD4 in multiple species

LBS	AA sequence	# species	Species
A104	SRFIVALKILF	2	Mus musculus, Rattus norvegicus
A104	SHFIVALKVLF	1	Homo sapiens
A104	TGYICALKVME	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
A104	TGLICALKAME	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
A104	SGFVCALKAME	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
A104	TGFIVALKTLH	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
A104	NKFIMALKVLF	1	Xenopus tropicalis
A157	LILEYAPRGEL	3	Homo sapiens, Mus musculus, Rattus norvegicus
A157	LLMEYLVNGEM	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
A157	LLMEYVYYGEL	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
A157	LLMEYLVNGEL	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
A157	LILEFAGRGEL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
A157	LMLEFAPRGEL	1	Xenopus tropicalis
E155	IYLILEYAPRG	3	Homo sapiens, Mus musculus, Rattus norvegicus
E155	VYLLMEYLVNG	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
E155	VYLLMEYVYYG	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
E155	IYLILEFAGRG	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
E155	IYLMLEFAPRG	1	Xenopus tropicalis
F88	LGKGKFGNVYL	4	Homo sapiens, Xenopus tropicalis, Mus musculus, Rattus norvegicus
F88	LGKGKFGKVYC	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
F88	LGKGKFGRVYC	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
F88	LGKGKFGRVYL	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
G160	EYAPRGELYKE	3	Homo sapiens, Mus musculus, Rattus norvegicus
G160	EYLVNGEMYKL	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
G160	EYVYYGELYKF	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
G160	EYLVNGELYKH	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
G160	EFAGRGELYQH	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
G160	EFAPRGELYKE	1	Xenopus tropicalis
K106	FIVALKILFKS	2	Mus musculus, Rattus norvegicus
K106	FIVALKVLFKS	1	Homo sapiens
K106	YICALKVMEKE	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
K106	LICALKAMEKK	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
K106	FVCALKAMEKK	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
K106	FIVALKTLHKS	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
K106	FIMALKVLFKS	1	Xenopus tropicalis
L207	KPENILIGFNN	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
L207	KPENLLLGLQG	2	Mus musculus, Rattus norvegicus
L207	KPENLLLGLKG	1	Homo sapiens
L207	KPENIIIGFNN	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
L207	KPENILLGIDG	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L207	KPENLLMGYKG	1	Xenopus tropicalis
L83	EIGRPLGKGKF	3	Homo sapiens, Mus musculus, Rattus norvegicus
L83	ELGKKLGKGKF	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
L83	EIGKILGKGKF	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
L83	EIGKVLGKGKF	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
L83	EIGKPLGKGKF	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
L83	DIGRPLGKGKF	1	Xenopus tropicalis
P158	ILEYAPRGELY	3	Homo sapiens, Mus musculus, Rattus norvegicus
P158	LMEYLVNGEMY	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
P158	LMEYVYYGELY	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
P158	LMEYLVNGELY	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
P158	ILEFAGRGELY	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
P158	MLEFAPRGELY	1	Xenopus tropicalis
V91	GKFGNVYLARE	3	Homo sapiens, Mus musculus, Rattus norvegicus
V91	GKFGKVYCVRH	1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
V91	GKFGKVYCVKH	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
V91	GKFGRVYCVRH	1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
V91	GKFGRVYLAKE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
V91	GKFGNVYLARD	1	Xenopus tropicalis
Y156	YLILEYAPRGE	3	Homo sapiens, Mus musculus, Rattus norvegicus
Y156	YLLMEYLVNGE	2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
Y156	YLLMEYVYYGE	1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Y156	YLILEFAGRGE	1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Y156	YLMLEFAPRGE	1	Xenopus tropicalis