| General information | Literature | Expression | Regulation | Mutation | Interaction |
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Basic Information |
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|---|---|
Gene ID | 22806 |
Name | IKZF3 |
Synonymous | AIO|AIOLOS|ZNFN1A3;IKAROS family zinc finger 3 (Aiolos);IKZF3;IKAROS family zinc finger 3 (Aiolos) |
Definition | zinc finger DNA binding protein Aiolos|zinc finger protein Aiolos|zinc finger protein, subfamily 1A, 3 (Aiolos) |
Position | 17q21 |
Gene type | protein-coding |
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Title |
Abstract |
| Overexpression of novel short isoforms of Helios in a patient with T-cell acute lymphoblastic leukemia. | OBJECTIVE: In previous studies, we demonstrated overexpression of the dominant-negative isoform of the transcription factor Ikaros, Ik-6, in patients with blast crisis of chronic myelogenous leukemia and B-cell acute lymphoblastic leukemia. In the present study, we analyzed expression of the Ikaros family genes Ikaros, Aiolos, and Helios in a panel of human T-cell leukemia/lymphoma cell lines and bone marrow samples of patients with T-cell acute lymphoblastic leukemia. MATERIALS AND METHODS: We performed reverse transcriptase polymerase chain reaction, sequencing analysis, immunoblotting, and Southern blotting. RESULTS: We found overexpression of novel short isoforms of Helios (Hel-5 and Hel-6) in the HD-Mar cell line. Southern blot analysis suggested that there might be a small deletion in the Helios locus of HD-Mar. In addition, we observed decreased expression of more than one Ikaros family gene in 3 of 9 patients with T-cell acute lymphoblastic leukemia. Moreover, one of the patients overexpressed novel short isoforms of Helios (Hel-7 and Hel-8). CONCLUSION: This study provides the first evidence of an Ikaros family member (other than Ikaros) of which novel short isoforms become overexpressed in human leukemia. |