407001

MIR218-2

MIRN218-2|mir-218-2;microRNA 218-2;MIR218-2;microRNA 218-2

hsa-mir-218-2

5q34

ncRNA

An increasing number of studies have suggested that microRNAs (miRNAs) are aberrantly expressed in numerous types of tumors and that a deregulation in miRNA expression may lead to carcinogenesis. Although miR218 has been demonstrated to be downregulated in several types of cancer, including medulloblastoma (MB), its involvement in MB is unclear. In the present study, the expression of miR218 and SH3GL1 were assessed in four MB cell lines and normal cerebellum by qPCR. The ectopic expression of miR218 induced by lentiviral transfection in MB cells on proliferation was evaluated by MTT assay, and cell migration and invasion were determined by transwell assays. Analysis of the target protein expression and related protein expression was determined by western blot analysis. The targeting of SH3GL1 by miR218 was identified using a luciferase reporter assay. The results demonstrated that miR218 was significantly downregulated in MB cell lines. MiR218 significantly inhibited SH3GL1 mRNA and protein expression and reduced the luciferase activity of a SH3GL1 3 untranslated regionbased reporter. Furthermore, overexpression of miR218 induced by transfection with lentivirus significantly suppressed MB cell growth, migration and invasion in vitro. Small interfering RNAmediated SH3GL1 downregulation partially phenocopied the effect of miR218 overexpression in the MB cell lines. The results indicated that miR218 was significantly downregulated in MB cancer cell lines. Furthermore, miR218 functioned as a tumor suppressor by regulating SH3GL1 expression in MB cancer cells.