Gene info: ID 4689

Gene: NCF4

GENE ID: 4689

Synonyms: CGD3|NCF|P40PHOX|SH3PXD4

Description: neutrophil cytosolic factor 4

Gene type: protein-coding

Location: 22q12.3

dbXrefs: Ensembl:ENSG00000100365 MIM:601488 Vega:OTTHUMG00000150548 UniprotKB:Q15080

RefSeq: NG_023400.1

Retrived from: PubMed

SNPs: 0 ANCO-SNPs

Phenotype: ND

# Publications: 3

Sherlock p-value : NA (AD) NA (ND) (Frontal_Cortex)

Pascal p-value : 2.863e-1 (AD) 6.750e-1 (ND)

DNA Methylation

Probe	Locus(GRCh37)	Gene	Region	p-val	Trait	PubMed ID
cg02532700	chr22:37257404	NCF4		1.010e-10	Maternal smoking in pregnancy	27040690
cg02532700	chr22:37257404	NCF4		5.200e-13	Smoking	27651444
cg02532700	chr22:37257404	NCF4		4.000e-12	Tobacco smoking	28225026
cg02532700	chr22:37257404	NCF4		2.240e-10	Smoking	28686328

Gene Expression in Different Tissues

Temporal and Spatial Expressions (BrainSpan)

Footnote:
SC: sub-cortical regions; SM: sensory-motor regions; FC: frontal cortex; and TP: temporal-parietal cortex
ST1: fetal (13 - 26 postconception weeks), ST2: early infancy to late childhood (4 months to 11 years), and ST3: adolescence to adulthood (13 - 23 years)
The bar shown representes average value of the expressions.

Top Co-expressed Genes in Brain-Frontal Cortex (BA9) (GTEx v7)

Top 10 positively co-expressed genes

Gene Symbol	Pearson Correlation Coefficient
MYO1F	0.946
CMTM7	0.933
HCK	0.93
PARVG	0.923
SASH3	0.921
LAIR1	0.921
FCGR2A	0.919
WAS	0.919
GPSM3	0.919
CSF2RB	0.918

Top 10 negatively co-expressed genes

Gene Symbol	Pearson Correlation Coefficient
KCNIP3	-0.531
ST6GAL2	-0.525
NEUROD6	-0.514
MARCH4	-0.504
TMEM179	-0.499
LRTM2	-0.499
SLC7A4	-0.495
ZSCAN1	-0.492
ATP2C2	-0.492
KREMEN2	-0.491

ID	Drug Name	Drug Type	CAS Num	Status	Role
DB00514	Dextromethorphan	Small Molecule	125-71-3	Approved	Target

ID	Drug Name	Action	PubMed
C010914	2,4-diaminotoluene	"2,4-diaminotoluene results in increased expression of NCF4 mRNA"	20713471
C009505	4,4'-diaminodiphenylmethane	"4,4'-diaminodiphenylmethane results in decreased expression of NCF4 mRNA"	18648102
D000082	Acetaminophen	Acetaminophen affects the expression of NCF4 mRNA	17562736
D000111	Acetylcysteine	Acetylcysteine inhibits the reaction [Cadmium Chloride results in increased expression of NCF4 protein]	21195169
D000111	Acetylcysteine	Acetylcysteine results in decreased expression of NCF4 protein	21195169
C031143	AICA ribonucleotide	AICA ribonucleotide inhibits the reaction [Cadmium Chloride results in increased expression of NCF4 protein]	21195169
D018501	Antirheumatic Agents	Antirheumatic Agents results in decreased expression of NCF4 mRNA	24449571
C547126	AZM551248	AZM551248 results in increased expression of NCF4 mRNA	22323515
C022921	benzo(k)fluoranthene	benzo(k)fluoranthene results in increased expression of NCF4 mRNA	26377693
D001564	Benzo(a)pyrene	Benzo(a)pyrene results in decreased expression of NCF4 mRNA	22316170
D001564	Benzo(a)pyrene	Benzo(a)pyrene results in increased expression of NCF4 mRNA	22228805
C006780	bisphenol A	bisphenol A affects the expression of NCF4 mRNA	21786754
C006780	bisphenol A	bisphenol A results in decreased expression of NCF4 mRNA	25181051
D000069286	Bortezomib	Bortezomib results in decreased expression of NCF4 mRNA	20977926
D020122	tert-Butylhydroperoxide	tert-Butylhydroperoxide results in increased expression of NCF4 mRNA	14729362
D020122	tert-Butylhydroperoxide	tert-Butylhydroperoxide results in increased expression of NCF4 mRNA	18804290
D019256	Cadmium Chloride	Cadmium Chloride results in increased expression of NCF4 protein	21195169
D019256	Cadmium Chloride	Acetylcysteine inhibits the reaction [Cadmium Chloride results in increased expression of NCF4 protein]	21195169
D019256	Cadmium Chloride	AICA ribonucleotide inhibits the reaction [Cadmium Chloride results in increased expression of NCF4 protein]	21195169
D019256	Cadmium Chloride	Cadmium Chloride results in increased expression of NCF4 protein	21195169
D019256	Cadmium Chloride	PTEN inhibits the reaction [Cadmium Chloride results in increased expression of NCF4 protein]	21195169
D019256	Cadmium Chloride	Sirolimus inhibits the reaction [Cadmium Chloride results in increased expression of NCF4 protein]	21195169
D019256	Cadmium Chloride	Cadmium Chloride results in increased expression of NCF4 mRNA	26409624
C037123	cadmium sulfate	cadmium sulfate affects the reaction [MTF1 affects the expression of NCF4 mRNA]	16221973
D002737	Chloroprene	Chloroprene results in decreased expression of NCF4 mRNA	23125180
D002794	Choline	[Methionine deficiency co-treated with Choline deficiency co-treated with Folic Acid deficiency] results in increased expression of NCF4 mRNA	20938992
C074702	chromium hexavalent ion	chromium hexavalent ion results in increased expression of NCF4 protein	21742780
D003042	Cocaine	Cocaine results in increased expression of NCF4 protein	18952886
D003471	Cuprizone	Cuprizone results in increased expression of NCF4 mRNA	27523638
D003633	Dichlorodiphenyl Dichloroethylene	Dichlorodiphenyl Dichloroethylene results in increased expression of NCF4 mRNA	25386960
D004008	Diclofenac	Diclofenac results in increased expression of NCF4 mRNA	26934552
C036042	dicyclohexyl phthalate	dicyclohexyl phthalate affects the expression of NCF4 mRNA	26924002
D004026	Dieldrin	Dieldrin results in decreased expression of NCF4 mRNA	23041725
D004997	Ethinyl Estradiol	Ethinyl Estradiol affects the expression of NCF4 mRNA	17555576
D004997	Ethinyl Estradiol	Ethinyl Estradiol results in increased expression of NCF4 mRNA	17942748
D004997	Ethinyl Estradiol	[Tetrachlorodibenzodioxin co-treated with Ethinyl Estradiol] results in increased expression of NCF4 mRNA	17942748
D004997	Ethinyl Estradiol	Ethinyl Estradiol results in increased expression of NCF4 mRNA	17557909
D005020	Ethyl Methanesulfonate	Ethyl Methanesulfonate results in increased expression of NCF4 mRNA	23649840
D005492	Folic Acid	[Methionine deficiency co-treated with Choline deficiency co-treated with Folic Acid deficiency] results in increased expression of NCF4 mRNA	20938992
D015759	Ionomycin	[Tetradecanoylphorbol Acetate co-treated with Ionomycin] results in decreased expression of NCF4 mRNA	25613284
C561695	(+)-JQ1 compound	(+)-JQ1 compound results in decreased expression of NCF4 mRNA	23086925
C482199	lipopolysaccharide, E coli O55-B5	"lipopolysaccharide, E coli O55-B5 results in increased expression of NCF4 mRNA"	24972896
D008070	Lipopolysaccharides	Lipopolysaccharides results in decreased expression of NCF4 mRNA	21645290
D008627	Mercuric Chloride	Mercuric Chloride results in decreased expression of NCF4 mRNA	26409624
D008715	Methionine	[Methionine deficiency co-treated with Choline deficiency co-treated with Folic Acid deficiency] results in increased expression of NCF4 mRNA	20938992
D008727	Methotrexate	Methotrexate results in decreased expression of NCF4 mRNA	17400583
D008727	Methotrexate	Methotrexate results in increased expression of NCF4 mRNA	21678067
C004925	methylmercuric chloride	methylmercuric chloride results in decreased expression of NCF4 mRNA	26409624
D037742	Nanotubes, Carbon	"Nanotubes, Carbon analog results in increased expression of NCF4 mRNA"	25554681
D037742	Nanotubes, Carbon	"Nanotubes, Carbon results in increased expression of NCF4 mRNA"	25554681
D009532	Nickel	Nickel results in increased expression of NCF4 mRNA	24768652\|2558310
C086401	pentabromodiphenyl ether	pentabromodiphenyl ether results in decreased expression of NCF4 mRNA	23948077
C006253	pirinixic acid	pirinixic acid results in decreased expression of NCF4 mRNA	17426115
D011441	Propylthiouracil	Propylthiouracil results in decreased expression of NCF4 mRNA	24780913
D012822	Silicon Dioxide	Silicon Dioxide results in increased expression of NCF4 mRNA	23221170
D019821	Simvastatin	Simvastatin results in decreased expression of NCF4 mRNA	16157767
D020123	Sirolimus	Sirolimus inhibits the reaction [Cadmium Chloride results in increased expression of NCF4 protein]	21195169
C016104	sodium bichromate	sodium bichromate results in decreased expression of NCF4 mRNA	17685462
D012999	Soman	Soman results in increased expression of NCF4 mRNA	19281266
D053260	Soot	Soot results in increased expression of NCF4 mRNA	26551751
D053260	Soot	Soot results in increased expression of NCF4 mRNA	28283350
D013629	Tamoxifen	Tamoxifen affects the expression of NCF4 mRNA	17555576
D013749	Tetrachlorodibenzodioxin	Tetrachlorodibenzodioxin affects the expression of NCF4 mRNA	21570461\|2637764
D013749	Tetrachlorodibenzodioxin	[Tetrachlorodibenzodioxin co-treated with Ethinyl Estradiol] results in increased expression of NCF4 mRNA	17942748
D013749	Tetrachlorodibenzodioxin	Tetrachlorodibenzodioxin results in increased expression of NCF4 mRNA	16120747\|2629044
D013755	Tetradecanoylphorbol Acetate	[Tetradecanoylphorbol Acetate co-treated with Ionomycin] results in decreased expression of NCF4 mRNA	25613284
C009495	titanium dioxide	titanium dioxide results in increased expression of NCF4 mRNA	23557971
D014028	Tobacco Smoke Pollution	Tobacco Smoke Pollution results in decreased methylation of NCF4 gene	26756918
D014028	Tobacco Smoke Pollution	Tobacco Smoke Pollution affects the expression of NCF4 mRNA	20133372
D014635	Valproic Acid	Valproic Acid results in increased methylation of NCF4 gene	29154799
C025643	vinclozolin	vinclozolin results in increased expression of NCF4 mRNA	23034163
C029297	vinylidene chloride	vinylidene chloride results in increased expression of NCF4 mRNA	26682919
D015054	Zymosan	NCF4 gene mutant form results in decreased uptake of Zymosan	16880254

GO/Pathway

GO ID	GO Term	Qualifier	Evidence	PubMed
GO:0005515	protein binding	-	IPI	9365277 11483497 12887891 15657040 16297854 16782902
GO:0016176	superoxide-generating NADPH oxidase activator activity	-	IBA	21873635
GO:0016176	superoxide-generating NADPH oxidase activator activity	-	IMP	8280052
GO:0032266	phosphatidylinositol-3-phosphate binding	-	IDA	11684018
GO:0035091	phosphatidylinositol binding	-	IBA	21873635
GO:0046983	protein dimerization activity	-	TAS	8280052

GO ID	GO Term	Qualifier	Evidence	PubMed
GO:0006801	superoxide metabolic process	-	IBA	21873635
GO:0006909	phagocytosis	-	IEA	-
GO:0006955	immune response	-	TAS	8280052
GO:0043085	positive regulation of catalytic activity	-	IEA	-
GO:0045730	respiratory burst	-	IEA	-
GO:0048010	vascular endothelial growth factor receptor signaling pathway	-	TAS	-
GO:0055114	oxidation-reduction process	-	TAS	8839867

GO ID	GO Term	Qualifier	Evidence	PubMed
GO:0005829	cytosol	-	IDA	8280052
GO:0005829	cytosol	-	TAS	-
GO:0010008	endosome membrane	-	IDA	11684018
GO:0016020	membrane	-	IDA	8280052
GO:0032010	phagolysosome	-	TAS	-
GO:0043020	NADPH oxidase complex	-	IBA	21873635
GO:0043020	NADPH oxidase complex	-	IDA	8280052

KEGG ID	KEGG Term
hsa04145	Phagosome
hsa04380	Osteoclast differentiation
hsa04670	Leukocyte transendothelial migration
hsa05140	Leishmaniasis

Reactome ID	Reactome Term	Evidence
R-HSA-1222556	ROS, RNS production in phagocytes	TAS
R-HSA-1236973	Cross-presentation of particulate exogenous antigens (phagosomes)	TAS
R-HSA-1236975	Antigen processing-Cross presentation	TAS
R-HSA-1280218	Adaptive Immune System	TAS
R-HSA-162582	Signal Transduction	TAS
R-HSA-168249	Innate Immune System	TAS
R-HSA-168256	Immune System	TAS
R-HSA-194138	Signaling by VEGF	TAS
R-HSA-194315	Signaling by Rho GTPases	TAS
R-HSA-195258	RHO GTPase Effectors	TAS
R-HSA-2262752	Cellular responses to stress	TAS
R-HSA-3299685	Detoxification of Reactive Oxygen Species	TAS
R-HSA-4420097	VEGFA-VEGFR2 Pathway	TAS
R-HSA-5668599	RHO GTPases Activate NADPH Oxidases	TAS
R-HSA-8953897	Cellular responses to external stimuli	TAS
R-HSA-9006934	Signaling by Receptor Tyrosine Kinases	TAS
R-HSA-983169	Class I MHC mediated antigen processing & presentation	TAS

Interactions (STRING v10.5, Interaction Score >= 0.400)

Publications (Co-occurrence of gene symbol strings and ANCO-Dependence terms)

PMID	Title (Year)	Author	Journal
22879519	The NOD2insC polymorphism is associated with worse outcome following ileal pouch-anal anastomosis for ulcerative colitis. (2013 Oct)	Tyler AD	Gut
22158027	Clinical and genetic risk factors for perianal Crohn's disease in a population-based cohort. (2012 Apr)	Eglinton TW	Am J Gastroenterol
22115380	Prediction of disease complication occurrence in Crohn's disease using phenotype and genotype parameters at diagnosis. (2011 Dec)	Mazor Y	J Crohns Colitis