PLK1
Entrez ID: 5347
Full name: polo like kinase 1
External links: HGNC UniprotKB Ensembl RefSeq COSMIC
Family: Other: PLK: PLK1
Chromosomal location: 16p12.2
Substructure location: Gatekeeper: 130 A-loop: 193...216 G-loop: 60...67 αC-helix: 86...88
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PLK1
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhib...
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGO1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, WEE1 and HNRNPU. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylation of NINL component of the centrosome leads to NINL dissociation from other centrosomal proteins. Involved in mitosis exit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1 and RACGAP1. Recruited at the central spindle by phosphorylating and docking PRC1 and KIF20A/MKLP2; creates its own docking sites on PRC1 and KIF20A/MKLP2 by mediating phosphorylation of sites subsequently recognized by the POLO box domains. Phosphorylates RACGAP1, thereby creating a docking site for the Rho GTP exchange factor ECT2 that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2. Plays a central role in G2/M transition of mitotic cell cycle by phosphorylating CCNB1, CDC25C, FOXM1, CENPU, PKMYT1/MYT1, PPP1R12A/MYPT1 and WEE1. Part of a regulatory circuit that promotes the activation of CDK1 by phosphorylating the positive regulator CDC25C and inhibiting the negative regulators WEE1 and PKMYT1/MYT1. Also acts by mediating phosphorylation of cyclin-B1 (CCNB1) on centrosomes in prophase. Phosphorylates FOXM1, a key mitotic transcription regulator, leading to enhance FOXM1 transcriptional activity. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating BUB1B/BUBR1, FBXO5/EMI1 and STAG2/SA2. PLK1 is high on non-attached kinetochores suggesting a role of PLK1 in kinetochore attachment or in spindle assembly checkpoint (SAC) regulation. Required for kinetochore localization of BUB1B. Regulates the dissociation of cohesin from chromosomes by phosphorylating cohesin subunits such as STAG2/SA2. Phosphorylates SGO1: required for spindle pole localization of isoform 3 of SGO1 and plays a role in regulating its centriole cohesion function. Mediates phosphorylation of FBXO5/EMI1, a negative regulator of the APC/C complex during prophase, leading to FBXO5/EMI1 ubiquitination and degradation by the proteasome. Acts as a negative regulator of p53 family members: phosphorylates TOPORS, leading to inhibit the sumoylation of p53/TP53 and simultaneously enhance the ubiquitination and subsequent degradation of p53/TP53. Phosphorylates the transactivation domain of the transcription factor p73/TP73, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Also required for recovery after DNA damage checkpoint and entry into mitosis. Phosphorylates MISP, leading to stabilization of cortical and astral microtubule attachments required for proper spindle positioning (PubMed:8991084, PubMed:11202906, PubMed:12207013, PubMed:12447691, PubMed:12524548, PubMed:12738781, PubMed:12852856, PubMed:12939256, PubMed:14532005, PubMed:14734534, PubMed:15070733, PubMed:15148369, PubMed:15469984, PubMed:16198290, PubMed:16247472, PubMed:16980960, PubMed:17081991, PubMed:17351640, PubMed:17376779, PubMed:17617734, PubMed:18174154, PubMed:18331714, PubMed:18418051, PubMed:18477460, PubMed:18521620, PubMed:18615013, PubMed:19160488, PubMed:19351716, PubMed:19468300, PubMed:19468302, PubMed:19473992, PubMed:19509060, PubMed:19597481, PubMed:23455478, PubMed:23509069). Together with MEIKIN, acts as a regulator of kinetochore function during meiosis I: required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage (By similarity). Phosphorylates CEP68 and is required for its degradation (PubMed:25503564). Regulates nuclear envelope breakdown during prophase by phosphorylating DCTN1 resulting in its localization in the nuclear envelope (PubMed:20679239). Phosphorylates the heat shock transcription factor HSF1, promoting HSF1 nuclear translocation upon heat shock (PubMed:15661742). Phosphorylates HSF1 also in the early mitotic period; this phosphorylation regulates HSF1 localization to the spindle pole, the recruitment of the SCF(BTRC) ubiquitin ligase complex induicing HSF1 degradation, and hence mitotic progression (PubMed:18794143). Regulates mitotic progression by phosphorylating RIOK2 (PubMed:21880710).
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GO - Biological processes (BP):
anaphase-promoting complex-dependent catabolic process, centrosome cycle, ciliary basal body-plasma membrane docking, establishment of mitotic spindle orientation, establishment of protein localization, female meiosis chromosome segregation, G2/M transition of mitotic cell cycle, homologous chromoso...
anaphase-promoting complex-dependent catabolic process, centrosome cycle, ciliary basal body-plasma membrane docking, establishment of mitotic spindle orientation, establishment of protein localization, female meiosis chromosome segregation, G2/M transition of mitotic cell cycle, homologous chromosome segregation, microtubule bundle formation, mitotic cell cycle, mitotic cytokinesis, mitotic nuclear envelope disassembly, mitotic sister chromatid segregation, mitotic spindle assembly checkpoint, negative regulation of apoptotic process, negative regulation of cyclin-dependent protein serine/threonine kinase activity, negative regulation of transcription by RNA polymerase II, nuclear envelope disassembly, peptidyl-serine phosphorylation, positive regulation of peptidyl-threonine phosphorylation, positive regulation of proteasomal ubiquitin-dependent protein catabolic process, positive regulation of protein localization to nucleus, positive regulation of proteolysis, positive regulation of ubiquitin protein ligase activity, positive regulation of ubiquitin-protein transferase activity, protein destabilization, protein localization to chromatin, protein localization to nuclear envelope, protein phosphorylation, protein ubiquitination, regulation of cell cycle, regulation of cell cycle G2/M phase transition, regulation of cytokinesis, regulation of G2/M transition of mitotic cell cycle, regulation of mitotic cell cycle, regulation of mitotic cell cycle phase transition, regulation of mitotic metaphase/anaphase transition, regulation of mitotic spindle assembly, regulation of protein binding, regulation of protein localization to cell cortex, signal transduction involved in G2 DNA damage checkpoint, sister chromatid cohesion, synaptonemal complex disassembly, ubiquitin-dependent protein catabolic process
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GO - Molecular function (MF):
anaphase-promoting complex binding, ATP binding, identical protein binding, kinase activity, magnesium ion binding, microtubule binding, protein kinase activity, protein kinase binding, protein serine/threonine kinase activity...
anaphase-promoting complex binding, ATP binding, identical protein binding, kinase activity, magnesium ion binding, microtubule binding, protein kinase activity, protein kinase binding, protein serine/threonine kinase activity
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GO - Cellular component (CC):
centriolar satellite, centriole, centrosome, microtubule cytoskeleton, spindle, spindle midzone, spindle pole, cytosol, condensed nuclear chromosome outer kinetochore, nucleoplasm, nucleus, synaptonemal complex, cell, chromatin, cytoplasm, kinetochore, midbody...
centriolar satellite, centriole, centrosome, microtubule cytoskeleton, spindle, spindle midzone, spindle pole, cytosol, condensed nuclear chromosome outer kinetochore, nucleoplasm, nucleus, synaptonemal complex, cell, chromatin, cytoplasm, kinetochore, midbody
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polo like kinase 1
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*NOTE: the Kaplan plots were collected from OncoLnc